Restriction map for
pFN21AE0961
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI ApaI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbrPI BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MroI MseI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Alw44I 424 GTGCAC G^TGCAC
AocI 1096 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1273 AGCGCT AGC^GCT
ApaLI 424 GTGCAC G^TGCAC
Asp700I 702 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 781 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 329 GGATCC G^GATCC
BanII 535 GAGCCC G[GA]GC[CT]^C
BbeI 1420 GGCGCC GGCGC^C
BcgI 1006 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 508 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 508 GCTAAGC GC^T[ACGT]AGC
BsgI 145 CTGCAC GTGCAG(16/14)
BsiEI 177 CGGCCG CG[GA][CT]^CG
Bsp1407I 629 TGTACA T^GTACA
BspHI 965 TCATGA T^CATGA
BsrDI 863 GCAATG GCAATG(2/0)
BsrGI 629 TGTACA T^GTACA
BssSI 1112 CTCGTG CACGAG(-5/-1)
BstXI 776 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1096 CCTGAGG CC^T[ACGT]AGG
CelII 508 GCTAAGC GC^T[ACGT]AGC
DraII 544 GGGACCC [GA]G^G[ACGT]CC[CT]
EagI 177 CGGCCG C^GGCCG
EclXI 177 CGGCCG C^GGCCG
Eco47III 1273 AGCGCT AGC^GCT
Eco52I 177 CGGCCG C^GGCCG
Eco81I 1096 CCTGAGG CC^T[ACGT]AGG
EcoO109I 544 GGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 593 GACGC GACGC(5/10)
KasI 1420 GGCGCC G^GCGCC
MaeII 371 ACGT A^CGT
MslI 960 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1420 GGCGCC GG^CGCC
PflFI 781 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 544 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 965 TCATGA T^CATGA
ScaI 281 AGTACT AGT^ACT
SfoI 1420 GGCGCC GGC^GCC
SmaI 1042 CCCGGG CCC^GGG
SspBI 629 TGTACA T^GTACA
TaqI 478 TCGA T^CGA
Tth111I 781 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 478 TCGA T^CGA
XmaCI 1042 CCCGGG C^CCGGG
XmaI 1042 CCCGGG C^CCGGG
XmnI 702 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 593 GACGCC G[GA]^CG[CT]C
1420 GGCGCC
AspHI 424 GTGCAC G[AT]GC[AT]^C
1320 GAGCAC
AvaI 550 CCCGAG C^[CT]CG[GA]G
1042 CCCGGG
AvaII 336 GGACC G^G[AT]CC
545 GGACC
BalI 302 TGGCCA TGG^CCA
1355 TGGCCA
BanI 981 GGCACC G^G[CT][GA]CC
1420 GGCGCC
BbvCI 1019 CCTCAGC CCTCAGC(-5/-2)
1088 CCTCAGC
BfaI 514 CTAG C^TAG
1445 CTAG
BsaHI 593 GACGCC G[GA]^CG[CT]C
1420 GGCGCC
BsiHKAI 424 GTGCAC G[AT]GC[AT]^C
1320 GAGCAC
BsmAI 359 GAGAC GTCTC(1/5)
1482 GTCTC
BsoBI 550 CCCGAG C^[CT]CG[GA]G
1042 CCCGGG
BspMI 151 ACCTGC ACCTGC(4/8)
1051 GCAGGT
BsrI 326 ACTGG ACTGG(1/-1)
1011 CCAGT
BstYI 329 GGATCC [GA]^GATC[CT]
716 AGATCC
CfoI 1274 GCGC GCG^C
1421 GCGC
DsaI 305 CCACGG C^C[GA][CT]GG
395 CCGTGG
EcoNI 333 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
525 CCTCATCAAGG
EcoT14I 122 CCAAGG C^C[AT][AT]GG
1361 CCAAGG
HaeII 1273 AGCGCT [GA]GCGC^[CT]
1420 GGCGCC
HhaI 1274 GCGC GCG^C
1421 GCGC
Hin1I 593 GACGCC G[GA]^CG[CT]C
1420 GGCGCC
HinP1I 1274 GCGC G^CGC
1421 GCGC
MaeI 514 CTAG C^TAG
1445 CTAG
MflI 329 GGATCC [GA]^GATC[CT]
716 AGATCC
MluNI 302 TGGCCA TGG^CCA
1355 TGGCCA
MscI 302 TGGCCA TGG^CCA
1355 TGGCCA
NaeI 578 GCCGGC GCC^GGC
1383 GCCGGC
NgoMIV 578 GCCGGC G^CCGGC
1383 GCCGGC
SexAI 449 ACCTGGT A^CC[AT]GGT
782 ACCTGGT
StyI 122 CCAAGG C^C[AT][AT]GG
1361 CCAAGG
TfiI 432 GAATC G^A[AT]TC
702 GAATC
XhoII 329 GGATCC [GA]^GATC[CT]
716 AGATCC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BcnI BglI BmyI BpmI
BsaBI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI Cac8I Cfr10I Cfr13I DdeI
DpnII EaeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MamI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TseI Tsp45I TspRI