Restriction map for
pFN21AE1309
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI Hin1I HindIII
KasI KspI MaeI MamI MflI
MroI NaeI NarI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RsrII SacII SbfI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 15 GGTACC G^GTACC
AccI 370 GTCGAC GT^[AC][GT]AC
AcsI 451 GAATTT [GA]^AATT[CT]
AflIII 716 ACGCGT A^C[GA][CT]GT
AlwI 555 GATCC GGATC(4/5)
ApaI 250 GGGCCC GGGCC^C
ApoI 451 GAATTT [GA]^AATT[CT]
Asp718I 15 GGTACC G^GTACC
AvaI 341 CCCGAG C^[CT]CG[GA]G
BanI 15 GGTACC G^G[CT][GA]CC
BbsI 759 GAAGAC GAAGAC(2/6)
BbvCI 191 CCTCAGC CCTCAGC(-5/-2)
BcgI 330 CGAGGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 752 TGATCA T^GATCA
BglI 4 GCCACACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 759 GAAGAC GAAGAC(2/6)
BsaI 970 GAGACC GGTCTC(1/5)
BsaWI 108 TCCGGT [AT]^CCGG[AT]
BseRI 29 CTCCTC GAGGAG(10/8)
BsgI 280 CTGCAC GTGCAG(16/14)
BsmBI 321 GAGACG CGTCTC(1/5)
BsoBI 341 CCCGAG C^[CT]CG[GA]G
BspHI 755 TCATGA T^CATGA
BspMI 763 ACCTGC ACCTGC(4/8)
BsrBI 224 CCGCTC CCGCTC(-3/-3)
DsaI 680 CCATGG C^C[GA][CT]GG
EarI 242 CTCTTC CTCTTC(1/4)
EcoT14I 680 CCATGG C^C[AT][AT]GG
FbaI 752 TGATCA T^GATCA
HgaI 323 GACGC GACGC(5/10)
HpaI 934 GTTAAC GTT^AAC
KpnI 15 GGTACC GGTAC^C
Ksp632I 242 CTCTTC CTCTTC(1/4)
MaeII 647 ACGT A^CGT
MfeI 396 CAATTG C^AATTG
MluI 716 ACGCGT A^CGCGT
MunI 396 CAATTG C^AATTG
NcoI 680 CCATGG C^CATGG
NotI 263 GCGGCCGC GC^GGCCGC
PpuMI 352 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 607 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 896 CAGCTG CAG^CTG
RcaI 755 TCATGA T^CATGA
SacI 499 GAGCTC GAGCT^C
SalI 370 GTCGAC G^TCGAC
SapI 241 GCTCTTC GCTCTTC(1/4)
ScaI 563 AGTACT AGT^ACT
SfcI 567 CTACAG C^T[GA][CT]AG
SgrAI 8 CACCGGCG C[GA]^CCGG[CT]G
SmlI 795 CTCAAG C^T[CT][GA]AG
StyI 680 CCATGG C^C[AT][AT]GG
TaqI 371 TCGA T^CGA
TthHB8I 371 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 268 CGCG CG^CG
717 CGCG
AlwNI 140 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
155 CAGCGGCTG
BanII 250 GGGCCC G[GA]GC[CT]^C
499 GAGCTC
BsiEI 264 CGGCCG CG[GA][CT]^CG
624 CGGCCG
BsrFI 9 ACCGGC [GA]^CCGG[CT]
96 ACCGGC
BstEII 390 GGTCACC G^GT[ACGT]ACC
1008 GGTGACC
BstPI 390 GGTCACC G^GT[ACGT]ACC
1008 GGTGACC
BstUI 268 CGCG CG^CG
717 CGCG
Cfr10I 9 ACCGGC [GA]^CCGG[CT]
96 ACCGGC
DpnII 555 GATC ^GATC
753 GATC
DraIII 379 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
544 CACTCTGTG
EagI 264 CGGCCG C^GGCCG
624 CGGCCG
EclXI 264 CGGCCG C^GGCCG
624 CGGCCG
Eco52I 264 CGGCCG C^GGCCG
624 CGGCCG
EcoO65I 390 GGTCACC G^GT[ACGT]ACC
1008 GGTGACC
FauI 115 CCCGC CCCGC(4/6)
223 CCCGC
HaeII 81 AGCGCC [GA]GCGC^[CT]
219 AGCGCC
HphI 1008 GGTGA GGTGA(8/7)
392 TCACC
MboI 555 GATC ^GATC
753 GATC
MseI 658 TTAA T^TAA
935 TTAA
MslI 654 CACTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
681 CATGGGAGTG
MvnI 268 CGCG CG^CG
717 CGCG
NdeII 555 GATC ^GATC
753 GATC
Sau3AI 555 GATC ^GATC
753 GATC
Tru9I 658 TTAA T^TAA
935 TTAA
Tsp509I 397 AATT ^AATT
452 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
ItaI MaeIII MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI