Restriction map for
pFN21AE3644
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BcgI
BcnI BfrI BglI BglII BlnI
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
FauI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MslI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AclI 1030 AACGTT AA^CGTT
AlwNI 122 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 621 GGACC G^G[AT]CC
BanI 1110 GGCACC G^G[CT][GA]CC
BanII 770 GGGCTC G[GA]GC[CT]^C
BbvCI 48 GCTGAGG CCTCAGC(-5/-2)
BciVI 1212 GGATAC GTATCC(6/5)
BmyI 770 GGGCTC G[AGT]GC[ACT]^C
BpmI 773 CTCCAG CTGGAG(16/14)
BsiEI 39 CGACCG CG[GA][CT]^CG
BsmI 1483 GCATTC GAATGC(1/-1)
Bsp1286I 770 GGGCTC G[AGT]GC[ACT]^C
BsrDI 1476 CATTGC GCAATG(2/0)
BsrFI 41 ACCGGC [GA]^CCGG[CT]
BssSI 923 CTCGTG CACGAG(-5/-1)
BstXI 904 CCATCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 4 GCGC GCG^C
Cfr10I 41 ACCGGC [GA]^CCGG[CT]
Cfr13I 621 GGACC G^G[ACGT]CC
EcoNI 970 CCTGTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1322 GATATC GAT^ATC
EcoT22I 533 ATGCAT ATGCA^T
HapII 42 CCGG C^CGG
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HpaII 42 CCGG C^CGG
MspI 42 CCGG C^CGG
NsiI 533 ATGCAT ATGCA^T
NspI 1571 GCATGT [GA]CATG^[CT]
PflMI 154 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 622 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1030 AACGTT AA^CGTT
Sau96I 621 GGACC G^G[ACGT]CC
SexAI 153 ACCAGGT A^CC[AT]GGT
SpeI 471 ACTAGT A^CTAGT
SspI 659 AATATT AAT^ATT
Tsp45I 1575 GTCAC ^GT[GC]AC
Van91I 154 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 336 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 1064 CCGC CCGC(-3/-1)
197 GCGG
Asp700I 328 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
580 GAACATTTTC
BalI 944 TGGCCA TGG^CCA
1104 TGGCCA
BclI 63 TGATCA T^GATCA
647 TGATCA
BlpI 413 GCTTAGC GC^T[ACGT]AGC
1462 GCTGAGC
Bpu1102I 413 GCTTAGC GC^T[ACGT]AGC
1462 GCTGAGC
BsaJI 266 CCATGG C^C[ACGT][ACGT]GG
637 CCATGG
BsiYI 154 CCAGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
970 CCTGTTCGAGG
BslI 154 CCAGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
970 CCTGTTCGAGG
BsmAI 298 GTCTC GTCTC(1/5)
1379 GTCTC
BstYI 543 GGATCT [GA]^GATC[CT]
839 AGATCC
CelII 413 GCTTAGC GC^T[ACGT]AGC
1462 GCTGAGC
DsaI 266 CCATGG C^C[GA][CT]GG
637 CCATGG
EaeI 944 TGGCCA [CT]^GGCC[GA]
1104 TGGCCA
EarI 316 GAAGAG CTCTTC(1/4)
784 GAAGAG
EcoT14I 266 CCATGG C^C[AT][AT]GG
637 CCATGG
FbaI 63 TGATCA T^GATCA
647 TGATCA
HaeIII 945 GGCC GG^CC
1105 GGCC
Ksp632I 316 GAAGAG CTCTTC(1/4)
784 GAAGAG
MaeII 706 ACGT A^CGT
1031 ACGT
MaeIII 1284 GTTAC ^GT[ACGT]AC
1575 GTCAC
MflI 543 GGATCT [GA]^GATC[CT]
839 AGATCC
MluNI 944 TGGCCA TGG^CCA
1104 TGGCCA
MscI 944 TGGCCA TGG^CCA
1104 TGGCCA
NcoI 266 CCATGG C^CATGG
637 CCATGG
NdeI 464 CATATG CA^TATG
607 CATATG
NlaIV 771 GGCTCC GG[ACGT]^[ACGT]CC
1110 GGCACC
PstI 1093 CTGCAG CTGCA^G
1149 CTGCAG
PvuII 7 CAGCTG CAG^CTG
46 CAGCTG
SfaNI 205 GATGC GCATC(5/9)
1057 GATGC
SmlI 22 CTCAAG C^T[CT][GA]AG
1505 CTCAAG
StyI 266 CCATGG C^C[AT][AT]GG
637 CCATGG
XhoII 543 GGATCT [GA]^GATC[CT]
839 AGATCC
XmnI 328 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
580 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsaBI BsrI BssKI
BstF5I BstNI DdeI DpnII EcoRI
EcoRII Fnu4HI FokI HinfI HphI
ItaI MaeI MamI MboI MboII
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII PleI RsaI Sau3AI
ScrFI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I