Restriction map for
pFN21AE4404
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# NO CUT ENZYMES ( 125 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BalI BamHI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BspEI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CpoI DraI DraIII DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI MvnI NcoI NdeI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 610 GACGTC GACGT^C
AflIII 1146 ACATGT A^C[GA][CT]GT
ApaI 688 GGGCCC GGGCC^C
AspHI 742 GAGCAC G[AT]GC[AT]^C
AvrII 909 CCTAGG C^CTAGG
BbeI 131 GGCGCC GGCGC^C
BbsI 492 GAAGAC GAAGAC(2/6)
BclI 143 TGATCA T^GATCA
BlnI 909 CCTAGG C^CTAGG
BpuAI 492 GAAGAC GAAGAC(2/6)
BsaAI 1054 TACGTG [CT]AC^GT[GA]
BseRI 1251 GAGGAG GAGGAG(10/8)
BsgI 1186 GTGCAG GTGCAG(16/14)
BsiHKAI 742 GAGCAC G[AT]GC[AT]^C
BsmBI 608 GAGACG CGTCTC(1/5)
Bsp1407I 503 TGTACA T^GTACA
BspDI 625 ATCGAT AT^CGAT
BspHI 983 TCATGA T^CATGA
BspLU11I 1146 ACATGT A^CATGT
BsrFI 551 GCCGGC [GA]^CCGG[CT]
BsrGI 503 TGTACA T^GTACA
CfoI 132 GCGC GCG^C
Cfr10I 551 GCCGGC [GA]^CCGG[CT]
ClaI 625 ATCGAT AT^CGAT
DraII 688 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 595 GACCGAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 264 TGGCCG [CT]^GGCC[GA]
EcoO109I 688 GGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 143 TGATCA T^GATCA
HaeII 131 GGCGCC [GA]GCGC^[CT]
HgaI 528 GCGTC GACGC(5/10)
HhaI 132 GCGC GCG^C
HinP1I 132 GCGC G^CGC
HincII 1000 GTTGAC GT[CT]^[GA]AC
HindII 1000 GTTGAC GT[CT]^[GA]AC
KasI 131 GGCGCC G^GCGCC
MaeIII 403 GTCAC ^GT[ACGT]AC
NaeI 551 GCCGGC GCC^GGC
NarI 131 GGCGCC GG^CGCC
NgoMIV 551 GCCGGC G^CCGGC
NheI 468 GCTAGC G^CTAGC
NspI 1146 ACATGT [GA]CATG^[CT]
RcaI 983 TCATGA T^CATGA
SfcI 1220 CTGTAG C^T[GA][CT]AG
SfoI 131 GGCGCC GGC^GCC
SgrAI 550 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1029 CCCGGG CCC^GGG
SspBI 503 TGTACA T^GTACA
StuI 906 AGGCCT AGG^CCT
Tsp45I 403 GTCAC ^GT[GC]AC
XmaCI 1029 CCCGGG C^CCGGG
XmaI 1029 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 1011 GGACC G^G[AT]CC
1041 GGACC
BanI 131 GGCGCC G^G[CT][GA]CC
1116 GGCACC
BanII 516 GGGCTC G[GA]GC[CT]^C
688 GGGCCC
BfaI 469 CTAG C^TAG
910 CTAG
BsmI 124 GCATTC GAATGC(1/-1)
656 GCATTC
BspMI 677 ACCTGC ACCTGC(4/8)
803 ACCTGC
DpnII 144 GATC ^GATC
1170 GATC
EcoRI 564 GAATTC G^AATTC
619 GAATTC
MaeI 469 CTAG C^TAG
910 CTAG
MboI 144 GATC ^GATC
1170 GATC
MboII 492 GAAGA GAAGA(8/7)
440 TCTTC
MspA1I 509 CAGCTG C[AC]G^C[GT]G
1078 CAGCTG
NdeII 144 GATC ^GATC
1170 GATC
PaeR7I 164 CTCGAG C^TCGAG
461 CTCGAG
PvuII 509 CAGCTG CAG^CTG
1078 CAGCTG
Sau3AI 144 GATC ^GATC
1170 GATC
SmlI 164 CTCGAG C^T[CT][GA]AG
461 CTCGAG
TfiI 336 GATTC G^A[AT]TC
397 GAATC
XhoI 164 CTCGAG C^TCGAG
461 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwNI ApoI AvaI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CelII Cfr13I DdeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII Hin1I HinfI HpaII
HphI ItaI MaeII MnlI MslI
MspI MvaI MwoI NciI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp509I
TspRI TthHB8I