Restriction map for
pFN21AE6205
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AluI 50 AGCT AG^CT
AspHI 154 GTGCTC G[AT]GC[AT]^C
BglII 192 AGATCT A^GATCT
BmyI 154 GTGCTC G[AGT]GC[ACT]^C
BseRI 188 GAGGAG GAGGAG(10/8)
BsiHKAI 154 GTGCTC G[AT]GC[AT]^C
BsiYI 439 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 439 CCTTATATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 154 GTGCTC G[AGT]GC[ACT]^C
BspMI 174 ACCTGC ACCTGC(4/8)
BsrI 127 ACTGG ACTGG(1/-1)
BssKI 425 CCAGG ^CC[ACGT]GG
BstF5I 35 GGATG GGATG(2/0)
BstNI 425 CCAGG CC^[AT]GG
BstXI 227 CCATACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 157 CTCAG C^T[ACGT]AG
EarI 266 GAAGAG CTCTTC(1/4)
EcoRII 425 CCAGG ^CC[AT]GG
FokI 35 GGATG GGATG(9/13)
HaeIII 370 GGCC GG^CC
HphI 285 TCACC GGTGA(8/7)
Ksp632I 266 GAAGAG CTCTTC(1/4)
MaeIII 454 GTGAC ^GT[ACGT]AC
MslI 228 CATACGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 425 CCAGG CC^[AT]GG
MwoI 312 GCAAAATGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 171 GGAACC GG[ACGT]^[ACGT]CC
PleI 290 GAGTC GAGTC(4/5)
ScrFI 425 CCAGG CC^[ACGT]GG
SfcI 278 CTATAG C^T[GA][CT]AG
StuI 369 AGGCCT AGG^CCT
TaqI 213 TCGA T^CGA
Tsp45I 454 GTGAC ^GT[GC]AC
TspRI 159 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 213 TCGA T^CGA
XbaI 9 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 69 AAATTT [GA]^AATT[CT]
481 AAATTT
AlwI 223 GATCC GGATC(4/5)
459 GATCC
ApoI 69 AAATTT [GA]^AATT[CT]
481 AAATTT
BbvI 48 GCAGC GCAGC(8/12)
164 GCAGC
BfaI 10 CTAG C^TAG
397 CTAG
BsaJI 366 CCGAGG C^C[ACGT][ACGT]GG
425 CCAGGG
BsmAI 6 GTCTC GTCTC(1/5)
85 GTCTC
BspHI 303 TCATGA T^CATGA
464 TCATGA
BstYI 192 AGATCT [GA]^GATC[CT]
222 AGATCC
MaeI 10 CTAG C^TAG
397 CTAG
MflI 192 AGATCT [GA]^GATC[CT]
222 AGATCC
MseI 115 TTAA T^TAA
485 TTAA
RcaI 303 TCATGA T^CATGA
464 TCATGA
TfiI 273 GATTC G^A[AT]TC
300 GATTC
Tru9I 115 TTAA T^TAA
485 TTAA
TseI 48 GCAGC G^C[AT]GC
164 GCAGC
XhoII 192 AGATCT [GA]^GATC[CT]
222 AGATCC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI DpnII Fnu4HI HinfI ItaI
MboI MboII MnlI NdeII NlaIII
Sau3AI Tsp509I