Restriction map for
pFN21AE1452
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AocI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Alw44I 601 GTGCAC G^TGCAC
AlwNI 191 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 344 AGCGCT AGC^GCT
ApaLI 601 GTGCAC G^TGCAC
Asp700I 693 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 601 GTGCAC G[AT]GC[AT]^C
BalI 2 TGGCCA TGG^CCA
BcgI 411 GCAGATACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 627 CCGGG CC^[GC]GG
BfaI 499 CTAG C^TAG
BmyI 601 GTGCAC G[AGT]GC[ACT]^C
BseRI 223 GAGGAG GAGGAG(10/8)
BsiHKAI 601 GTGCAC G[AT]GC[AT]^C
BsmFI 629 GGGAC GGGAC(10/14)
Bsp1286I 601 GTGCAC G[AGT]GC[ACT]^C
BspMI 80 ACCTGC ACCTGC(4/8)
BsrBI 226 GAGCGG CCGCTC(-3/-3)
BssSI 604 CACGAG CACGAG(-5/-1)
BstAPI 594 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 390 GGTCACC G^GT[ACGT]ACC
BstPI 390 GGTCACC G^GT[ACGT]ACC
BstYI 653 AGATCC [GA]^GATC[CT]
Cac8I 138 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 345 GCGC GCG^C
DraI 129 TTTAAA TTT^AAA
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eco47III 344 AGCGCT AGC^GCT
EcoO65I 390 GGTCACC G^GT[ACGT]ACC
EcoT14I 35 CCAAGG C^C[AT][AT]GG
EcoT22I 122 ATGCAT ATGCA^T
HaeII 344 AGCGCT [GA]GCGC^[CT]
HhaI 345 GCGC GCG^C
HinP1I 345 GCGC G^CGC
MaeI 499 CTAG C^TAG
MflI 653 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 130 TTAA T^TAA
NciI 627 CCGGG CC^[GC]GG
NsiI 122 ATGCAT ATGCA^T
PpuMI 291 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 286 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
StyI 35 CCAAGG C^C[AT][AT]GG
TaqI 701 TCGA T^CGA
Tru9I 130 TTAA T^TAA
Tsp509I 439 AATT ^AATT
TthHB8I 701 TCGA T^CGA
XhoII 653 AGATCC [GA]^GATC[CT]
XmnI 693 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 657 CCGC CCGC(-3/-1)
228 GCGG
AfaI 65 GTAC GT^AC
624 GTAC
AlwI 199 GATCC GGATC(4/5)
654 GATCC
AvaI 22 CCCGAG C^[CT]CG[GA]G
85 CTCGGG
BsoBI 22 CCCGAG C^[CT]CG[GA]G
85 CTCGGG
BsrDI 317 GCAATG GCAATG(2/0)
659 GCAATG
DraII 173 AGGCCCC [GA]G^G[ACGT]CC[CT]
291 AGGTCCT
EcoO109I 173 AGGCCCC [GA]G^G[ACGT]CC[CT]
291 AGGTCCT
HapII 627 CCGG C^CGG
636 CCGG
HpaII 627 CCGG C^CGG
636 CCGG
MslI 186 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
577 CATGTGGGTG
MspA1I 406 CAGCTG C[AC]G^C[GT]G
595 CAGCTG
MspI 627 CCGG C^CGG
636 CCGG
PstI 340 CTGCAG CTGCA^G
409 CTGCAG
PvuII 406 CAGCTG CAG^CTG
595 CAGCTG
RsaI 65 GTAC GT^AC
624 GTAC
SexAI 386 ACCTGGT A^CC[AT]GGT
397 ACCTGGT
SfaNI 121 GATGC GCATC(5/9)
547 GATGC
SfcI 340 CTGCAG C^T[GA][CT]AG
409 CTGCAG
SmlI 159 CTTGAG C^T[CT][GA]AG
709 CTCAAG
Tsp45I 391 GTCAC ^GT[GC]AC
553 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AvaII BbsI BbvI BpuAI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HphI
ItaI MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI TspRI