Restriction map for
pFN21AE5708
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspHI AvaI AviII AvrII BamHI
BanII BbeI BbvCI BcgI BcnI
BfrI BlnI BlpI BmyI BpmI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspEI BspMI BsrGI BssHII
BssSI BstEII BstPI BstUI CelII
CfoI CpoI DraII DraIII Eco47III
EcoNI EcoO109I EcoO65I EcoRV FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SapI SbfI ScaI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AcsI 250 GAATTC [GA]^AATT[CT]
AfaI 640 GTAC GT^AC
AhdI 498 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1648 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1396 CCTCAGG CC^T[ACGT]AGG
ApoI 250 GAATTC [GA]^AATT[CT]
AseI 757 ATTAAT AT^TAAT
AsnI 757 ATTAAT AT^TAAT
AspEI 498 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2108 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 505 GGTCC G^G[AT]CC
BanI 107 GGCACC G^G[CT][GA]CC
BbrPI 392 CACGTG CAC^GTG
BciVI 1958 GGATAC GTATCC(6/5)
BclI 1739 TGATCA T^GATCA
BglI 2063 GCCGGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 282 AGATCT A^GATCT
BsaAI 392 CACGTG [CT]AC^GT[GA]
BsgI 1670 GTGCAG GTGCAG(16/14)
BsiEI 2094 CGGCCG CG[GA][CT]^CG
BsmI 1239 GAATGC GAATGC(1/-1)
BspDI 2174 ATCGAT AT^CGAT
BspHI 924 TCATGA T^CATGA
BspLU11I 855 ACATGT A^CATGT
BsrBI 627 CCGCTC CCGCTC(-3/-3)
BsrDI 1263 CATTGC GCAATG(2/0)
Bst1107I 997 GTATAC GTA^TAC
BstBI 767 TTCGAA TT^CGAA
BstZ17I 997 GTATAC GTA^TAC
Bsu36I 1396 CCTCAGG CC^T[ACGT]AGG
ClaI 2174 ATCGAT AT^CGAT
DrdI 2117 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1347 CCGTGG C^C[GA][CT]GG
EagI 2094 CGGCCG C^GGCCG
Eam1105I 498 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2094 CGGCCG C^GGCCG
Eco52I 2094 CGGCCG C^GGCCG
Eco81I 1396 CCTCAGG CC^T[ACGT]AGG
EcoRI 250 GAATTC G^AATTC
EcoT22I 1679 ATGCAT ATGCA^T
FbaI 1739 TGATCA T^GATCA
HgaI 1430 GACGC GACGC(5/10)
MslI 109 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1803 CAGCTG C[AC]G^C[GT]G
NsiI 1679 ATGCAT ATGCA^T
NspI 855 ACATGT [GA]CATG^[CT]
NspV 767 TTCGAA TT^CGAA
PflFI 2108 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 392 CACGTG CAC^GTG
PmlI 392 CACGTG CAC^GTG
PshBI 757 ATTAAT AT^TAAT
PvuII 1803 CAGCTG CAG^CTG
RcaI 924 TCATGA T^CATGA
RsaI 640 GTAC GT^AC
SfiI 2062 GGCCGGTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 767 TTCGAA TT^CGAA
SmlI 536 CTCAAG C^T[CT][GA]AG
SspI 1997 AATATT AAT^ATT
Tth111I 2108 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 627 CCGC CCGC(-3/-1)
2093 GCGG
AlwI 837 GGATC GGATC(4/5)
876 GGATC
BalI 1094 TGGCCA TGG^CCA
1214 TGGCCA
BbsI 949 GAAGAC GAAGAC(2/6)
2153 GAAGAC
BpuAI 949 GAAGAC GAAGAC(2/6)
2153 GAAGAC
BsmFI 23 GGGAC GGGAC(10/14)
1259 GGGAC
BstAPI 1672 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1681 GCATTGGCTGC
BstYI 282 AGATCT [GA]^GATC[CT]
837 GGATCT
Cac8I 1122 GCCAGC GC[ACGT]^[ACGT]GC
1971 GCCAGC
Cfr13I 505 GGTCC G^G[ACGT]CC
2190 GGGCC
DraI 1315 TTTAAA TTT^AAA
1531 TTTAAA
EarI 436 GAAGAG CTCTTC(1/4)
1660 GAAGAG
EcoT14I 47 CCTTGG C^C[AT][AT]GG
176 CCAAGG
HindIII 448 AAGCTT A^AGCTT
1719 AAGCTT
Ksp632I 436 GAAGAG CTCTTC(1/4)
1660 GAAGAG
MaeII 393 ACGT A^CGT
1536 ACGT
MflI 282 AGATCT [GA]^GATC[CT]
837 GGATCT
MluNI 1094 TGGCCA TGG^CCA
1214 TGGCCA
MscI 1094 TGGCCA TGG^CCA
1214 TGGCCA
PstI 820 CTGCAG CTGCA^G
1688 CTGCAG
SalI 2114 GTCGAC G^TCGAC
2138 GTCGAC
Sau96I 505 GGTCC G^G[ACGT]CC
2190 GGGCC
SexAI 798 ACCTGGT A^CC[AT]GGT
1373 ACCTGGT
StyI 47 CCTTGG C^C[AT][AT]GG
176 CCAAGG
XhoII 282 AGATCT [GA]^GATC[CT]
837 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccI AflIII AluI BbvI BfaI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsrFI BsrI BssKI BstF5I
BstNI BstXI Cfr10I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI