Restriction map for
pFN21AE1342
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII ItaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MslI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TseI
TspRI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 328 GTAGAC GT^[AC][GT]AC
AciI 79 CCGC CCGC(-3/-1)
AfaI 531 GTAC GT^AC
AflIII 437 ACACGT A^C[GA][CT]GT
AluI 258 AGCT AG^CT
AlwI 413 GGATC GGATC(4/5)
AlwNI 142 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 214 ATTAAT AT^TAAT
AsnI 214 ATTAAT AT^TAAT
Asp700I 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 257 GAGCTC G[AT]GC[AT]^C
AspI 331 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 63 GGACC G^G[AT]CC
BalI 73 TGGCCA TGG^CCA
BanII 257 GAGCTC G[GA]GC[CT]^C
BbsI 165 GAAGAC GAAGAC(2/6)
BfaI 280 CTAG C^TAG
BmyI 257 GAGCTC G[AGT]GC[ACT]^C
BpuAI 165 GAAGAC GAAGAC(2/6)
BseRI 514 GAGGAG GAGGAG(10/8)
BsiHKAI 257 GAGCTC G[AT]GC[AT]^C
Bsp1286I 257 GAGCTC G[AGT]GC[ACT]^C
BspMI 65 ACCTGC ACCTGC(4/8)
BsrBI 79 CCGCTC CCGCTC(-3/-3)
Cfr13I 63 GGACC G^G[ACGT]CC
DpnII 414 GATC ^GATC
DraI 201 TTTAAA TTT^AAA
DraII 62 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 73 TGGCCA [CT]^GGCC[GA]
EarI 123 CTCTTC CTCTTC(1/4)
EcoNI 247 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 62 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 91 GAATTC G^AATTC
Ksp632I 123 CTCTTC CTCTTC(1/4)
MaeI 280 CTAG C^TAG
MaeII 439 ACGT A^CGT
MboI 414 GATC ^GATC
MluNI 73 TGGCCA TGG^CCA
MscI 73 TGGCCA TGG^CCA
NdeII 414 GATC ^GATC
PflFI 331 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 36 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 62 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 214 ATTAAT AT^TAAT
RsaI 531 GTAC GT^AC
SacI 257 GAGCTC GAGCT^C
Sau3AI 414 GATC ^GATC
Sau96I 63 GGACC G^G[ACGT]CC
SexAI 169 ACCTGGT A^CC[AT]GGT
SmlI 238 CTCAAG C^T[CT][GA]AG
TaqI 195 TCGA T^CGA
Tth111I 331 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 195 TCGA T^CGA
Van91I 36 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 279 TCTAGA T^CTAGA
XmnI 87 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 8 enzymes) #
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AcsI 91 GAATTC [GA]^AATT[CT]
535 AAATTT
ApoI 91 GAATTC [GA]^AATT[CT]
535 AAATTT
BsmFI 62 GGGAC GGGAC(10/14)
33 GTCCC
HaeIII 74 GGCC GG^CC
388 GGCC
HphI 150 GGTGA GGTGA(8/7)
381 TCACC
MaeIII 406 GTGAC ^GT[ACGT]AC
434 GTGAC
NlaIV 62 GGGACC GG[ACGT]^[ACGT]CC
243 GGAACC
Tsp45I 406 GTGAC ^GT[GC]AC
434 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BsaJI BsiYI BslI BssKI BstNI
DdeI EcoRII HinfI MboII MnlI
MseI MvaI NlaIII ScrFI SfaNI
TfiI Tru9I Tsp509I