##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HapII       HgaI        HincII      HindII      HindIII     
HpaI        HpaII       HphI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        NaeI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccII          65  CGCG                 CG^CG
AcyI           31  GGCGCC               G[GA]^CG[CT]C
AlwNI         260  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanI           31  GGCGCC               G^G[CT][GA]CC
BanII          46  GAGCCC               G[GA]GC[CT]^C
BbeI           31  GGCGCC               GGCGC^C
BglI          187  GCCGAAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI           46  GAGCCC               G[AGT]GC[ACT]^C
BsaHI          31  GGCGCC               G[GA]^CG[CT]C
BsaJI          38  CCAAGG               C^C[ACGT][ACGT]GG
Bsp1286I       46  GAGCCC               G[AGT]GC[ACT]^C
BspMI         222  ACCTGC               ACCTGC(4/8)
BstUI          65  CGCG                 CG^CG
DdeI          248  CTGAG                C^T[ACGT]AG
DpnII          59  GATC                 ^GATC
EarI           43  GAAGAG               CTCTTC(1/4)
EcoT14I        38  CCAAGG               C^C[AT][AT]GG
HaeII          31  GGCGCC               [GA]GCGC^[CT]
HaeIII        186  GGCC                 GG^CC
Hin1I          31  GGCGCC               G[GA]^CG[CT]C
HinfI         255  GAGTC                G^A[ACGT]TC
KasI           31  GGCGCC               G^GCGCC
Ksp632I        43  GAAGAG               CTCTTC(1/4)
MboI           59  GATC                 ^GATC
MvnI           65  CGCG                 CG^CG
NarI           31  GGCGCC               GG^CGCC
NdeII          59  GATC                 ^GATC
NlaIV          31  GGCGCC               GG[ACGT]^[ACGT]CC
PleI          255  GAGTC                GAGTC(4/5)
SapI           43  GAAGAGC              GCTCTTC(1/4)
Sau3AI         59  GATC                 ^GATC
SfoI           31  GGCGCC               GGC^GCC
StyI           38  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI           19  AGCT                 AG^CT
               75  AGCT                
BbsI          214  GAAGAC               GAAGAC(2/6)
              243  GAAGAC              
BbvI          115  GCAGC                GCAGC(8/12)
              118  GCAGC               
BpuAI         214  GAAGAC               GAAGAC(2/6)
              243  GAAGAC              
BseRI          54  GAGGAG               GAGGAG(10/8)
              250  GAGGAG              
CfoI           32  GCGC                 GCG^C
               64  GCGC                
HhaI           32  GCGC                 GCG^C
               64  GCGC                
HinP1I         32  GCGC                 G^CGC
               64  GCGC                
MwoI           25  GCCGAAGGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              187  GCCGAAGTGGC         
TseI          115  GCAGC                G^C[AT]GC
              118  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AciI        Fnu4HI      ItaI        MboII       MnlI