Restriction map for
pFN21AE4455
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AviII AvrII BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DrdI Eam1105I
EarI Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI Ksp632I KspI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SfiI SfuI
SgrAI SpeI SphI SspI StuI
SwaI Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 160 GTCTAC GT^[AC][GT]AC
AcsI 628 AAATTC [GA]^AATT[CT]
AflIII 332 ACACGT A^C[GA][CT]GT
Aor51HI 1379 AGCGCT AGC^GCT
ApoI 628 AAATTC [GA]^AATT[CT]
AvaI 727 CCCGGG C^[CT]CG[GA]G
BamHI 530 GGATCC G^GATCC
BanII 781 GAGCTC G[GA]GC[CT]^C
BbeI 722 GGCGCC GGCGC^C
BbsI 876 GAAGAC GAAGAC(2/6)
BfaI 43 CTAG C^TAG
BglI 1031 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 876 GAAGAC GAAGAC(2/6)
BsaBI 660 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 454 CTCCTC GAGGAG(10/8)
BsgI 988 GTGCAG GTGCAG(16/14)
BsiEI 1365 CGGCCG CG[GA][CT]^CG
BsoBI 727 CCCGGG C^[CT]CG[GA]G
Bsp1407I 329 TGTACA T^GTACA
BsrFI 205 ACCGGC [GA]^CCGG[CT]
BsrGI 329 TGTACA T^GTACA
BstEII 309 GGTCACC G^GT[ACGT]ACC
BstPI 309 GGTCACC G^GT[ACGT]ACC
BstYI 530 GGATCC [GA]^GATC[CT]
Cfr10I 205 ACCGGC [GA]^CCGG[CT]
DraII 1073 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 1365 CGGCCG C^GGCCG
EclXI 1365 CGGCCG C^GGCCG
Eco47III 1379 AGCGCT AGC^GCT
Eco52I 1365 CGGCCG C^GGCCG
EcoO109I 1073 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 309 GGTCACC G^GT[ACGT]ACC
HincII 817 GTCAAC GT[CT]^[GA]AC
HindII 817 GTCAAC GT[CT]^[GA]AC
KasI 722 GGCGCC G^GCGCC
MaeI 43 CTAG C^TAG
MamI 660 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 530 GGATCC [GA]^GATC[CT]
MseI 674 TTAA T^TAA
NarI 722 GGCGCC GG^CGCC
NspI 234 ACATGC [GA]CATG^[CT]
PpuMI 1073 AGGACCT [GA]G^G[AT]CC[CT]
SacI 781 GAGCTC GAGCT^C
SexAI 865 ACCTGGT A^CC[AT]GGT
SfoI 722 GGCGCC GGC^GCC
SmaI 727 CCCGGG CCC^GGG
SmlI 74 CTCAAG C^T[CT][GA]AG
SnaBI 373 TACGTA TAC^GTA
SspBI 329 TGTACA T^GTACA
TfiI 1418 GAATC G^A[AT]TC
Tru9I 674 TTAA T^TAA
XbaI 42 TCTAGA T^CTAGA
XcmI 575 CCACCTTCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 530 GGATCC [GA]^GATC[CT]
XmaCI 727 CCCGGG C^CCGGG
XmaI 727 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 869 GGTACC G^GTACC
911 GGTACC
AlwNI 618 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
961 CAGGGTCTG
Asp718I 869 GGTACC G^GTACC
911 GGTACC
AspI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
928 GACTGTGTC
BalI 1058 TGGCCA TGG^CCA
1307 TGGCCA
BsaAI 373 TACGTA [CT]AC^GT[GA]
838 TACGTG
BsmBI 335 CGTCTC CGTCTC(1/5)
570 CGTCTC
BsmFI 1337 GGGAC GGGAC(10/14)
1370 GGGAC
BspMI 464 ACCTGC ACCTGC(4/8)
1277 ACCTGC
DraIII 982 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
1006 CACGCCGTG
HphI 53 TCACC GGTGA(8/7)
311 TCACC
KpnI 869 GGTACC GGTAC^C
911 GGTACC
MluNI 1058 TGGCCA TGG^CCA
1307 TGGCCA
MscI 1058 TGGCCA TGG^CCA
1307 TGGCCA
NcoI 584 CCATGG C^CATGG
806 CCATGG
PflFI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
928 GACTGTGTC
PvuII 192 CAGCTG CAG^CTG
991 CAGCTG
SbfI 406 CCTGCAGG CCTGCA^GG
825 CCTGCAGG
Sse8387I 406 CCTGCAGG CCTGCA^GG
825 CCTGCAGG
Tsp509I 629 AATT ^AATT
640 AATT
Tth111I 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
928 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BbvI BcnI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I