Restriction map for
pFN21AE4409
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII AccI AccII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BciVI BfrI BglII BlnI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FseI FspI HgaI HincII HindII
HpaI KspI MfeI MluI MluNI
MscI MseI MunI MvnI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 648 GGTACC G^GTACC
AccIII 218 TCCGGA T^CCGGA
AcyI 92 GGCGCC G[GA]^CG[CT]C
Asp718I 648 GGTACC G^GTACC
AvaI 611 CTCGGG C^[CT]CG[GA]G
BbeI 92 GGCGCC GGCGC^C
BbvCI 1216 CCTCAGC CCTCAGC(-5/-2)
BcgI 889 GCAGAGAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 810 TGATCA T^GATCA
BglI 820 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1159 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1159 GCTGAGC GC^T[ACGT]AGC
BsaBI 595 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 92 GGCGCC G[GA]^CG[CT]C
BsaI 530 GAGACC GGTCTC(1/5)
BsaWI 218 TCCGGA [AT]^CCGG[AT]
BseAI 218 TCCGGA T^CCGGA
BsgI 206 CTGCAC GTGCAG(16/14)
BsiEI 81 CGACCG CG[GA][CT]^CG
BsoBI 611 CTCGGG C^[CT]CG[GA]G
BspEI 218 TCCGGA T^CCGGA
BspMI 1102 GCAGGT ACCTGC(4/8)
BsrBI 222 GAGCGG CCGCTC(-3/-3)
BstXI 369 CCATCTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1159 GCTGAGC GC^T[ACGT]AGC
CfoI 93 GCGC GCG^C
DraII 1150 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 449 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 879 CCACGG C^C[GA][CT]GG
EarI 1117 CTCTTC CTCTTC(1/4)
EcoNI 567 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1150 AGGGCCC [GA]G^G[ACGT]CC[CT]
FbaI 810 TGATCA T^GATCA
HaeII 92 GGCGCC [GA]GCGC^[CT]
HhaI 93 GCGC GCG^C
Hin1I 92 GGCGCC G[GA]^CG[CT]C
HinP1I 93 GCGC G^CGC
HindIII 464 AAGCTT A^AGCTT
HinfI 227 GAATC G^A[ACGT]TC
KasI 92 GGCGCC G^GCGCC
KpnI 648 GGTACC GGTAC^C
Ksp632I 1117 CTCTTC CTCTTC(1/4)
MamI 595 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 218 TCCGGA T^CCGGA
MspA1I 203 CAGCTG C[AC]G^C[GT]G
NarI 92 GGCGCC GG^CGCC
NheI 979 GCTAGC G^CTAGC
PflMI 1197 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 203 CAGCTG CAG^CTG
SacI 1162 GAGCTC GAGCT^C
SfaNI 595 GATGC GCATC(5/9)
SfcI 773 CTACAG C^T[GA][CT]AG
SfoI 92 GGCGCC GGC^GCC
SmlI 63 CTTGAG C^T[CT][GA]AG
StuI 923 AGGCCT AGG^CCT
TfiI 227 GAATC G^A[AT]TC
Tsp509I 473 AATT ^AATT
Van91I 1197 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1079 CCAGTGTAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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ApaI 1151 GGGCCC GGGCC^C
1206 GGGCCC
AvaII 883 GGACC G^G[AT]CC
1073 GGACC
BbsI 550 GAAGAC GAAGAC(2/6)
250 GTCTTC
BcnI 75 CCGGG CC^[GC]GG
1049 CCGGG
BpuAI 550 GAAGAC GAAGAC(2/6)
250 GTCTTC
BsaAI 715 TACGTG [CT]AC^GT[GA]
1123 CACGTA
BseRI 142 GAGGAG GAGGAG(10/8)
1040 GAGGAG
BsmFI 614 GGGAC GGGAC(10/14)
1250 GGGAC
BsmI 53 GCATTC GAATGC(1/-1)
1108 GCATTC
BspHI 591 TCATGA T^CATGA
813 TCATGA
BstYI 29 GGATCT [GA]^GATC[CT]
1200 GGATCT
HphI 328 GGTGA GGTGA(8/7)
190 TCACC
MflI 29 GGATCT [GA]^GATC[CT]
1200 GGATCT
NciI 75 CCGGG CC^[GC]GG
1049 CCGGG
NspI 539 ACATGC [GA]CATG^[CT]
1170 GCATGT
RcaI 591 TCATGA T^CATGA
813 TCATGA
TaqI 125 TCGA T^CGA
426 TCGA
TthHB8I 125 TCGA T^CGA
426 TCGA
XhoII 29 GGATCT [GA]^GATC[CT]
1200 GGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanI BanII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HpaII ItaI MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI