Restriction map for
pFN21AE6244
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpmI BsaAI BsaBI BsaI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HinfI HpaI HphI KpnI MamI
MfeI MluI MluNI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 718 GACGTC GACGT^C
AccIII 333 TCCGGA T^CCGGA
AlwI 503 GGATC GGATC(4/5)
AlwNI 676 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 379 GGGCCC GGGCC^C
BbeI 559 GGCGCC GGCGC^C
BbsI 412 GTCTTC GAAGAC(2/6)
BcnI 747 CCGGG CC^[GC]GG
BfaI 437 CTAG C^TAG
BglI 152 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 57 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 57 GCTGAGC GC^T[ACGT]AGC
BpuAI 412 GTCTTC GAAGAC(2/6)
BsaWI 333 TCCGGA [AT]^CCGG[AT]
BseAI 333 TCCGGA T^CCGGA
BseRI 621 CTCCTC GAGGAG(10/8)
BsmAI 283 GTCTC GTCTC(1/5)
BsmBI 282 CGTCTC CGTCTC(1/5)
BspEI 333 TCCGGA T^CCGGA
BssHII 101 GCGCGC G^CGCGC
BssSI 730 CTCGTG CACGAG(-5/-1)
BstYI 503 GGATCT [GA]^GATC[CT]
CelII 57 GCTGAGC GC^T[ACGT]AGC
DdeI 58 CTGAG C^T[ACGT]AG
EcoNI 318 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 83 CCAAGG C^C[AT][AT]GG
HgaI 410 GCGTC GACGC(5/10)
HindIII 204 AAGCTT A^AGCTT
KasI 559 GGCGCC G^GCGCC
KspI 23 CCGCGG CCGC^GG
MaeI 437 CTAG C^TAG
MaeII 719 ACGT A^CGT
MaeIII 667 GTCAC ^GT[ACGT]AC
MflI 503 GGATCT [GA]^GATC[CT]
MroI 333 TCCGGA T^CCGGA
MseI 551 TTAA T^TAA
NaeI 825 GCCGGC GCC^GGC
NarI 559 GGCGCC GG^CGCC
NciI 747 CCGGG CC^[GC]GG
NgoMIV 825 GCCGGC G^CCGGC
PstI 256 CTGCAG CTGCA^G
PvuII 55 CAGCTG CAG^CTG
SacII 23 CCGCGG CCGC^GG
SapI 369 GCTCTTC GCTCTTC(1/4)
ScaI 212 AGTACT AGT^ACT
SfcI 256 CTGCAG C^T[GA][CT]AG
SfoI 559 GGCGCC GGC^GCC
StyI 83 CCAAGG C^C[AT][AT]GG
TaqI 233 TCGA T^CGA
Tru9I 551 TTAA T^TAA
Tsp45I 667 GTCAC ^GT[GC]AC
TthHB8I 233 TCGA T^CGA
XhoII 503 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Aor51HI 338 AGCGCT AGC^GCT
431 AGCGCT
Asp700I 642 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
645 GAAGTTCTTC
AvaI 403 CTCGGG C^[CT]CG[GA]G
511 CCCGAG
BanI 559 GGCGCC G^G[CT][GA]CC
588 GGTGCC
BanII 223 GGGCTC G[GA]GC[CT]^C
379 GGGCCC
BsiEI 68 CGGCCG CG[GA][CT]^CG
159 CGGCCG
BsoBI 403 CTCGGG C^[CT]CG[GA]G
511 CCCGAG
BsrFI 425 ACCGGC [GA]^CCGG[CT]
825 GCCGGC
BstF5I 300 CATCC GGATG(2/0)
690 CATCC
Cfr10I 425 ACCGGC [GA]^CCGG[CT]
825 GCCGGC
DpnII 351 GATC ^GATC
504 GATC
EagI 68 CGGCCG C^GGCCG
159 CGGCCG
EarI 370 CTCTTC CTCTTC(1/4)
612 CTCTTC
EclXI 68 CGGCCG C^GGCCG
159 CGGCCG
Eco47III 338 AGCGCT AGC^GCT
431 AGCGCT
Eco52I 68 CGGCCG C^GGCCG
159 CGGCCG
FokI 300 CATCC GGATG(9/13)
690 CATCC
Ksp632I 370 CTCTTC CTCTTC(1/4)
612 CTCTTC
MboI 351 GATC ^GATC
504 GATC
MslI 441 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
447 CATGACAGTG
NdeII 351 GATC ^GATC
504 GATC
Sau3AI 351 GATC ^GATC
504 GATC
SfaNI 182 GCATC GCATC(5/9)
299 GCATC
XcmI 446 CCATGACAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
593 CCATCTTCCCCGTGG
XmnI 642 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
645 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BbvI BmyI BsaHI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NlaIII
NlaIV PpuMI RsaI Sau96I ScrFI
TseI TspRI