Restriction map for
pFN21AE3585
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsiEI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 372 CGCG CG^CG
AcyI 3 GGCGCC G[GA]^CG[CT]C
AgeI 600 ACCGGT A^CCGGT
AlwNI 1556 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 451 CCTGAGG CC^T[ACGT]AGG
Asp700I 165 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1176 GGGCTC G[GA]GC[CT]^C
BbeI 3 GGCGCC GGCGC^C
BcgI 374 CGAAGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1355 AGATCT A^GATCT
BpmI 80 CTGGAG CTGGAG(16/14)
BsaBI 636 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsgI 701 GTGCAG GTGCAG(16/14)
BsmBI 796 GAGACG CGTCTC(1/5)
BspDI 250 ATCGAT AT^CGAT
BsrDI 1457 GCAATG GCAATG(2/0)
BsrFI 600 ACCGGT [GA]^CCGG[CT]
BssSI 693 CACGAG CACGAG(-5/-1)
BstEII 243 GGTCACC G^GT[ACGT]ACC
BstPI 243 GGTCACC G^GT[ACGT]ACC
BstUI 372 CGCG CG^CG
Bsu36I 451 CCTGAGG CC^T[ACGT]AGG
Cfr10I 600 ACCGGT [GA]^CCGG[CT]
ClaI 250 ATCGAT AT^CGAT
DraII 338 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 235 GACCTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 385 CCATGG C^C[GA][CT]GG
Eco81I 451 CCTGAGG CC^T[ACGT]AGG
EcoO109I 338 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 243 GGTCACC G^GT[ACGT]ACC
EcoRV 121 GATATC GAT^ATC
Hin1I 3 GGCGCC G[GA]^CG[CT]C
KasI 3 GGCGCC G^GCGCC
MamI 636 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 632 TTAA T^TAA
MvnI 372 CGCG CG^CG
NarI 3 GGCGCC GG^CGCC
NcoI 385 CCATGG C^CATGG
NheI 907 GCTAGC G^CTAGC
PflMI 765 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 600 ACCGGT A^CCGGT
PstI 1553 CTGCAG CTGCA^G
ScaI 101 AGTACT AGT^ACT
SfoI 3 GGCGCC GGC^GCC
SgrAI 599 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1331 CCCGGG CCC^GGG
Tru9I 632 TTAA T^TAA
Van91I 765 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 297 TCTAGA T^CTAGA
XcmI 27 CCAGCGGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1331 CCCGGG C^CCGGG
XmaI 1331 CCCGGG C^CCGGG
XmnI 165 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 819 GGACC G^G[AT]CC
987 GGTCC
BalI 39 TGGCCA TGG^CCA
1467 TGGCCA
BbsI 540 GAAGAC GAAGAC(2/6)
619 GTCTTC
BpuAI 540 GAAGAC GAAGAC(2/6)
619 GTCTTC
BstXI 188 CCACTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1533 CCAGGAGGATGG
EaeI 39 TGGCCA [CT]^GGCC[GA]
1467 TGGCCA
FauI 30 GCGGG CCCGC(4/6)
816 GCGGG
MaeII 799 ACGT A^CGT
893 ACGT
MluNI 39 TGGCCA TGG^CCA
1467 TGGCCA
MscI 39 TGGCCA TGG^CCA
1467 TGGCCA
MslI 208 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
597 CACACCGGTG
PvuII 305 CAGCTG CAG^CTG
704 CAGCTG
SfcI 788 CTGTAG C^T[GA][CT]AG
1553 CTGCAG
Tsp509I 52 AATT ^AATT
870 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI BanI BbvI BcnI BfaI
BmyI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII