Restriction map for
pFN21AE2741
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
Acc65I AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsgI BsiEI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FbaI FseI
FspI HindIII HinfI HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 580 GACGTC GACGT^C
AccIII 903 TCCGGA T^CCGGA
AcyI 580 GACGTC G[GA]^CG[CT]C
AlwNI 835 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 574 CCTGAGG CC^T[ACGT]AGG
AspHI 247 GAGCAC G[AT]GC[AT]^C
AvaI 25 CCCGGG C^[CT]CG[GA]G
AvaII 1002 GGTCC G^G[AT]CC
BanI 910 GGCACC G^G[CT][GA]CC
BglI 676 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 580 GACGTC G[GA]^CG[CT]C
BsaWI 903 TCCGGA [AT]^CCGG[AT]
BseAI 903 TCCGGA T^CCGGA
BseRI 307 GAGGAG GAGGAG(10/8)
BsiHKAI 247 GAGCAC G[AT]GC[AT]^C
BsiWI 239 CGTACG C^GTACG
BsmAI 404 GAGAC GTCTC(1/5)
BsmFI 23 GTCCC GGGAC(10/14)
BsmI 222 GCATTC GAATGC(1/-1)
BsoBI 25 CCCGGG C^[CT]CG[GA]G
BspEI 903 TCCGGA T^CCGGA
BspMI 587 ACCTGC ACCTGC(4/8)
BsrBI 565 CCGCTC CCGCTC(-3/-3)
BstAPI 834 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 695 CCAAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 574 CCTGAGG CC^T[ACGT]AGG
DdeI 575 CTGAG C^T[ACGT]AG
DraII 1001 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 574 CCTGAGG CC^T[ACGT]AGG
EcoNI 197 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1001 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 666 GATATC GAT^ATC
HaeII 29 GGCGCT [GA]GCGC^[CT]
HgaI 798 GCGTC GACGC(5/10)
Hin1I 580 GACGTC G[GA]^CG[CT]C
HincII 583 GTCAAC GT[CT]^[GA]AC
HindII 583 GTCAAC GT[CT]^[GA]AC
MroI 903 TCCGGA T^CCGGA
MslI 1029 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 929 ACATGC [GA]CATG^[CT]
PpuMI 1001 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 292 CTGCAG CTGCA^G
PvuII 835 CAGCTG CAG^CTG
SfaNI 474 GATGC GCATC(5/9)
SfcI 292 CTGCAG C^T[GA][CT]AG
SmaI 25 CCCGGG CCC^GGG
TaqI 217 TCGA T^CGA
TthHB8I 217 TCGA T^CGA
XcmI 914 CCATTCTAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 25 CCCGGG C^CCGGG
XmaI 25 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BalI 125 TGGCCA TGG^CCA
887 TGGCCA
BanII 819 GGGCTC G[GA]GC[CT]^C
952 GAGCCC
BbsI 234 GAAGAC GAAGAC(2/6)
973 GAAGAC
BcnI 25 CCCGG CC^[GC]GG
26 CCGGG
BfaI 172 CTAG C^TAG
988 CTAG
BpuAI 234 GAAGAC GAAGAC(2/6)
973 GAAGAC
BsrDI 63 CATTGC GCAATG(2/0)
860 GCAATG
BstYI 317 GGATCT [GA]^GATC[CT]
397 GGATCT
Cfr13I 692 GGGCC G^G[ACGT]CC
1002 GGTCC
EarI 162 GAAGAG CTCTTC(1/4)
1018 GAAGAG
EcoRI 175 GAATTC G^AATTC
358 GAATTC
HapII 26 CCGG C^CGG
904 CCGG
HpaII 26 CCGG C^CGG
904 CCGG
Ksp632I 162 GAAGAG CTCTTC(1/4)
1018 GAAGAG
MaeI 172 CTAG C^TAG
988 CTAG
MflI 317 GGATCT [GA]^GATC[CT]
397 GGATCT
MluNI 125 TGGCCA TGG^CCA
887 TGGCCA
MscI 125 TGGCCA TGG^CCA
887 TGGCCA
MseI 185 TTAA T^TAA
983 TTAA
MspA1I 4 CCGCTG C[AC]G^C[GT]G
835 CAGCTG
MspI 26 CCGG C^CGG
904 CCGG
NciI 25 CCCGG CC^[GC]GG
26 CCGGG
Sau96I 692 GGGCC G^G[ACGT]CC
1002 GGTCC
Tru9I 185 TTAA T^TAA
983 TTAA
XhoII 317 GGATCT [GA]^GATC[CT]
397 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI DpnII
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HphI
ItaI MaeII MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI ScrFI StyI
TseI Tsp45I Tsp509I TspRI