Restriction map for
pFN21AB8596
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI MvnI
NarI NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2151 GTCTAC GT^[AC][GT]AC
AccIII 57 TCCGGA T^CCGGA
AflIII 2556 ACGTGT A^C[GA][CT]GT
AlwNI 779 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1240 CCTCAGG CC^T[ACGT]AGG
AseI 841 ATTAAT AT^TAAT
AsnI 841 ATTAAT AT^TAAT
AspHI 2331 GAGCTC G[AT]GC[AT]^C
BanII 2331 GAGCTC G[GA]GC[CT]^C
BmyI 2331 GAGCTC G[AGT]GC[ACT]^C
BseAI 57 TCCGGA T^CCGGA
BseRI 1495 GAGGAG GAGGAG(10/8)
BsgI 2068 GTGCAG GTGCAG(16/14)
BsiHKAI 2331 GAGCTC G[AT]GC[AT]^C
BsmAI 2049 GTCTC GTCTC(1/5)
Bsp1286I 2331 GAGCTC G[AGT]GC[ACT]^C
BspEI 57 TCCGGA T^CCGGA
BsrFI 1768 GCCGGC [GA]^CCGG[CT]
BstAPI 2424 GCATTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1240 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1768 GCCGGC [GA]^CCGG[CT]
DraII 814 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1559 GACTCTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 162 CCATGG C^C[GA][CT]GG
Eco81I 1240 CCTCAGG CC^T[ACGT]AGG
EcoO109I 814 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 162 CCATGG C^C[AT][AT]GG
EcoT22I 426 ATGCAT ATGCA^T
HpaI 2523 GTTAAC GTT^AAC
HphI 1614 TCACC GGTGA(8/7)
MroI 57 TCCGGA T^CCGGA
NaeI 1768 GCCGGC GCC^GGC
NcoI 162 CCATGG C^CATGG
NgoMIV 1768 GCCGGC G^CCGGC
NsiI 426 ATGCAT ATGCA^T
PflMI 1915 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 814 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 841 ATTAAT AT^TAAT
SacI 2331 GAGCTC GAGCT^C
SapI 1077 GAAGAGC GCTCTTC(1/4)
SfaNI 229 GATGC GCATC(5/9)
StyI 162 CCATGG C^C[AT][AT]GG
Van91I 1915 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 1036 CTTAAG C^TTAAG
1563 CTTAAG
AvaII 815 GGACC G^G[AT]CC
1924 GGACC
BanI 337 GGTGCC G^G[CT][GA]CC
2448 GGTGCC
BcnI 537 CCGGG CC^[GC]GG
1050 CCGGG
BfrI 1036 CTTAAG C^TTAAG
1563 CTTAAG
BglII 1124 AGATCT A^GATCT
1602 AGATCT
BsaJI 162 CCATGG C^C[ACGT][ACGT]GG
1888 CCAGGG
BsaWI 57 TCCGGA [AT]^CCGG[AT]
802 TCCGGT
BsmFI 814 GGGAC GGGAC(10/14)
1557 GGGAC
Cac8I 789 GCGAGC GC[ACGT]^[ACGT]GC
1768 GCCGGC
EarI 445 GAAGAG CTCTTC(1/4)
1077 GAAGAG
HincII 249 GTCAAC GT[CT]^[GA]AC
2523 GTTAAC
HindII 249 GTCAAC GT[CT]^[GA]AC
2523 GTTAAC
HindIII 931 AAGCTT A^AGCTT
2135 AAGCTT
Ksp632I 445 GAAGAG CTCTTC(1/4)
1077 GAAGAG
MslI 654 CACTGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2460 CATGGCAATG
MspA1I 80 CAGCTG C[AC]G^C[GT]G
377 CAGCTG
NciI 537 CCGGG CC^[GC]GG
1050 CCGGG
NdeI 31 CATATG CA^TATG
1660 CATATG
PleI 1559 GACTC GAGTC(4/5)
2299 GACTC
PstI 464 CTGCAG CTGCA^G
2528 CTGCAG
PvuII 80 CAGCTG CAG^CTG
377 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbsI BbvI BfaI BpmI
BpuAI BsiYI BslI BsmI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScaI ScrFI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI