Restriction map for
pFN21AB4222
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI AhdI AlwNI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbrPI BbsI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI FseI FspI
HindIII KpnI KspI MluI MroI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SgrAI SmlI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1800 GACGTC GACGT^C
Alw44I 2652 GTGCAC G^TGCAC
Aor51HI 359 AGCGCT AGC^GCT
ApaLI 2652 GTGCAC G^TGCAC
Asp700I 911 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 556 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2582 CCCGGG C^[CT]CG[GA]G
BalI 2424 TGGCCA TGG^CCA
BanII 2980 GGGCTC G[GA]GC[CT]^C
BbeI 2470 GGCGCC GGCGC^C
BciVI 2507 GTATCC GTATCC(6/5)
BclI 2132 TGATCA T^GATCA
BglII 167 AGATCT A^GATCT
BsaBI 1926 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1473 ACCGGA [AT]^CCGG[AT]
BseRI 163 GAGGAG GAGGAG(10/8)
BsgI 294 CTGCAC GTGCAG(16/14)
BsmBI 2554 GAGACG CGTCTC(1/5)
BsoBI 2582 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1848 TGTACA T^GTACA
BsrGI 1848 TGTACA T^GTACA
BstEII 145 GGTGACC G^GT[ACGT]ACC
BstPI 145 GGTGACC G^GT[ACGT]ACC
EaeI 2424 TGGCCA [CT]^GGCC[GA]
Eco47III 359 AGCGCT AGC^GCT
EcoNI 1261 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 145 GGTGACC G^GT[ACGT]ACC
EcoRV 529 GATATC GAT^ATC
EcoT22I 334 ATGCAT ATGCA^T
FauI 1859 GCGGG CCCGC(4/6)
FbaI 2132 TGATCA T^GATCA
HgaI 328 GACGC GACGC(5/10)
HpaI 840 GTTAAC GTT^AAC
KasI 2470 GGCGCC G^GCGCC
MamI 1926 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2424 TGGCCA TGG^CCA
MscI 2424 TGGCCA TGG^CCA
NarI 2470 GGCGCC GG^CGCC
NsiI 334 ATGCAT ATGCA^T
PflFI 556 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1219 GAAGAGC GCTCTTC(1/4)
SfcI 710 CTACAG C^T[GA][CT]AG
SfoI 2470 GGCGCC GGC^GCC
SmaI 2582 CCCGGG CCC^GGG
SnaBI 1465 TACGTA TAC^GTA
SspBI 1848 TGTACA T^GTACA
Tth111I 556 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1412 CCATTGATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2582 CCCGGG C^CCGGG
XmaI 2582 CCCGGG C^CCGGG
XmnI 911 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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BfaI 1153 CTAG C^TAG
2047 CTAG
BsaAI 1328 TACGTG [CT]AC^GT[GA]
1465 TACGTA
BsmI 63 GAATGC GAATGC(1/-1)
2183 GCATTC
BsrDI 1716 CATTGC GCAATG(2/0)
2144 GCAATG
BstBI 1238 TTCGAA TT^CGAA
1646 TTCGAA
DraII 373 GGGACCC [GA]G^G[ACGT]CC[CT]
2006 AGGACCT
DsaI 534 CCGTGG C^C[GA][CT]GG
897 CCGTGG
EarI 349 CTCTTC CTCTTC(1/4)
1219 GAAGAG
EcoO109I 373 GGGACCC [GA]G^G[ACGT]CC[CT]
2006 AGGACCT
EcoT14I 379 CCAAGG C^C[AT][AT]GG
1200 CCTTGG
HincII 840 GTTAAC GT[CT]^[GA]AC
1140 GTTGAC
HindII 840 GTTAAC GT[CT]^[GA]AC
1140 GTTGAC
Ksp632I 349 CTCTTC CTCTTC(1/4)
1219 GAAGAG
MaeI 1153 CTAG C^TAG
2047 CTAG
MfeI 830 CAATTG C^AATTG
2696 CAATTG
MslI 2541 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2632 CATAGACGTG
MunI 830 CAATTG C^AATTG
2696 CAATTG
NspV 1238 TTCGAA TT^CGAA
1646 TTCGAA
PflMI 315 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
379 CCAAGGAGTGG
PpuMI 373 GGGACCC [GA]G^G[AT]CC[CT]
2006 AGGACCT
PvuII 368 CAGCTG CAG^CTG
2228 CAGCTG
SfuI 1238 TTCGAA TT^CGAA
1646 TTCGAA
StyI 379 CCAAGG C^C[AT][AT]GG
1200 CCTTGG
Van91I 315 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
379 CCAAGGAGTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
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AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BamHI BanI BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII