Restriction map for
pFN21AE3522
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII
BstPI BstXI CelII CfoI Cfr10I
ClaI CpoI DraII EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI MfeI
MluI MroI MspI MunI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SbfI
SexAI SfiI SfoI SgrAI SmaI
SpeI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 1327 GGTACC G^GTACC
AccII 1866 CGCG CG^CG
Alw44I 1977 GTGCAC G^TGCAC
AlwI 1897 GGATC GGATC(4/5)
AlwNI 1574 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1699 CCTCAGG CC^T[ACGT]AGG
ApaLI 1977 GTGCAC G^TGCAC
Asp718I 1327 GGTACC G^GTACC
AspI 102 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 362 GGTCC G^G[AT]CC
BalI 1562 TGGCCA TGG^CCA
BglII 955 AGATCT A^GATCT
BsaBI 1892 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 777 CTCCTC GAGGAG(10/8)
BsiEI 101 CGACCG CG[GA][CT]^CG
BsmFI 1478 GGGAC GGGAC(10/14)
BsmI 1843 GAATGC GAATGC(1/-1)
BspHI 1146 TCATGA T^CATGA
BssKI 808 CCTGG ^CC[ACGT]GG
Bst1107I 438 GTATAC GTA^TAC
BstBI 485 TTCGAA TT^CGAA
BstNI 808 CCTGG CC^[AT]GG
BstUI 1866 CGCG CG^CG
BstYI 955 AGATCT [GA]^GATC[CT]
BstZ17I 438 GTATAC GTA^TAC
Bsu36I 1699 CCTCAGG CC^T[ACGT]AGG
Cfr13I 362 GGTCC G^G[ACGT]CC
DraI 903 TTTAAA TTT^AAA
DrdI 1043 GACAATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1562 TGGCCA [CT]^GGCC[GA]
EarI 160 GAAGAG CTCTTC(1/4)
Eco81I 1699 CCTCAGG CC^T[ACGT]AGG
EcoNI 1695 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 852 GAATTC G^AATTC
EcoRII 808 CCTGG ^CC[AT]GG
HincII 663 GTCGAC GT[CT]^[GA]AC
HindII 663 GTCGAC GT[CT]^[GA]AC
KpnI 1327 GGTACC GGTAC^C
Ksp632I 160 GAAGAG CTCTTC(1/4)
KspI 1865 CCGCGG CCGC^GG
MamI 1892 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 955 AGATCT [GA]^GATC[CT]
MluNI 1562 TGGCCA TGG^CCA
MscI 1562 TGGCCA TGG^CCA
MvaI 808 CCTGG CC^[AT]GG
MvnI 1866 CGCG CG^CG
NspV 485 TTCGAA TT^CGAA
PflFI 102 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 921 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 34 CAGCTG CAG^CTG
RcaI 1146 TCATGA T^CATGA
SacI 2071 GAGCTC GAGCT^C
SacII 1865 CCGCGG CCGC^GG
SalI 663 GTCGAC G^TCGAC
SapI 160 GAAGAGC GCTCTTC(1/4)
Sau96I 362 GGTCC G^G[ACGT]CC
ScaI 732 AGTACT AGT^ACT
ScrFI 808 CCTGG CC^[ACGT]GG
SfuI 485 TTCGAA TT^CGAA
SnaBI 1603 TACGTA TAC^GTA
SphI 165 GCATGC GCATG^C
Tth111I 102 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 921 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 135 CCATGTTGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 955 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 438 GTATAC GT^[AC][GT]AC
663 GTCGAC
AciI 1865 CCGC CCGC(-3/-1)
1867 GCGG
AflIII 24 ACATGT A^C[GA][CT]GT
1432 ACATGT
AspHI 1977 GTGCAC G[AT]GC[AT]^C
2071 GAGCTC
BbsI 948 GAAGAC GAAGAC(2/6)
2101 GAAGAC
BciVI 12 GGATAC GTATCC(6/5)
464 GGATAC
BfaI 4 CTAG C^TAG
941 CTAG
BpuAI 948 GAAGAC GAAGAC(2/6)
2101 GAAGAC
BsaAI 15 TACGTG [CT]AC^GT[GA]
1603 TACGTA
BsgI 333 CTGCAC GTGCAG(16/14)
1714 CTGCAC
BsiHKAI 1977 GTGCAC G[AT]GC[AT]^C
2071 GAGCTC
BsiYI 921 CCAATGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1695 CCTACCTCAGG
BslI 921 CCAATGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1695 CCTACCTCAGG
BspLU11I 24 ACATGT A^CATGT
1432 ACATGT
BsrDI 7 GCAATG GCAATG(2/0)
1955 GCAATG
DraIII 82 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1219 CACTCAGTG
EcoT14I 365 CCATGG C^C[AT][AT]GG
1881 CCATGG
HaeIII 794 GGCC GG^CC
1563 GGCC
HgaI 1267 GACGC GACGC(5/10)
2104 GACGC
MaeI 4 CTAG C^TAG
941 CTAG
MspA1I 34 CAGCTG C[AC]G^C[GT]G
1865 CCGCGG
NcoI 365 CCATGG C^CATGG
1881 CCATGG
NheI 3 GCTAGC G^CTAGC
940 GCTAGC
PstI 37 CTGCAG CTGCA^G
1812 CTGCAG
SfcI 37 CTGCAG C^T[GA][CT]AG
1812 CTGCAG
StyI 365 CCATGG C^C[AT][AT]GG
1881 CCATGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BanI BanII BbvI BmyI BpmI
BsaI BsaJI BsmAI Bsp1286I BsrI
BstF5I Cac8I DdeI DpnII DsaI
Fnu4HI FokI HindIII HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI SfaNI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI