Restriction map for
pFN21AB7560
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI MamI
MfeI MluI MroI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tsp45I TspRI Tth111I Van91I XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 215 GGTACC G^GTACC
AluI 758 AGCT AG^CT
AlwNI 541 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 215 GGTACC G^GTACC
BalI 458 TGGCCA TGG^CCA
BcgI 597 CGAGGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 103 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 52 GTGCCC G[AGT]GC[ACT]^C
BsaI 442 GAGACC GGTCTC(1/5)
BsiEI 113 CGGCCG CG[GA][CT]^CG
BsmFI 559 GGGAC GGGAC(10/14)
Bsp1286I 52 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 506 TGTACA T^GTACA
BspMI 418 ACCTGC ACCTGC(4/8)
BsrGI 506 TGTACA T^GTACA
BsrI 571 ACTGG ACTGG(1/-1)
BssSI 447 CACGAG CACGAG(-5/-1)
BstAPI 413 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI 715 CTAAG C^T[ACGT]AG
DraII 542 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 113 CGGCCG C^GGCCG
EclXI 113 CGGCCG C^GGCCG
Eco52I 113 CGGCCG C^GGCCG
EcoO109I 542 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 92 CCTTGG C^C[AT][AT]GG
HinfI 345 GACTC G^A[ACGT]TC
HphI 131 TCACC GGTGA(8/7)
KpnI 215 GGTACC GGTAC^C
MaeII 587 ACGT A^CGT
MaeIII 711 GTTAC ^GT[ACGT]AC
MluNI 458 TGGCCA TGG^CCA
MscI 458 TGGCCA TGG^CCA
NlaIII 408 CATG CATG^
NotI 112 GCGGCCGC GC^GGCCGC
PaeR7I 595 CTCGAG C^TCGAG
PleI 345 GACTC GAGTC(4/5)
PstI 435 CTGCAG CTGCA^G
SapI 531 GAAGAGC GCTCTTC(1/4)
SfaNI 144 GATGC GCATC(5/9)
SfcI 435 CTGCAG C^T[GA][CT]AG
SmaI 181 CCCGGG CCC^GGG
SmlI 595 CTCGAG C^T[CT][GA]AG
SspBI 506 TGTACA T^GTACA
StyI 92 CCTTGG C^C[AT][AT]GG
XbaI 330 TCTAGA T^CTAGA
XcmI 446 CCACGAGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 595 CTCGAG C^TCGAG
XmaCI 181 CCCGGG C^CCGGG
XmaI 181 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 216 GTAC GT^AC
507 GTAC
AvaII 270 GGACC G^G[AT]CC
288 GGACC
BseRI 247 GAGGAG GAGGAG(10/8)
340 GAGGAG
BsgI 420 CTGCAC GTGCAG(16/14)
605 GTGCAG
BsmAI 343 GAGAC GTCTC(1/5)
442 GAGAC
BstF5I 237 GGATG GGATG(2/0)
479 GGATG
BstYI 327 GGATCT [GA]^GATC[CT]
643 GGATCT
EaeI 113 CGGCCG [CT]^GGCC[GA]
458 TGGCCA
EarI 244 GAAGAG CTCTTC(1/4)
531 GAAGAG
FokI 237 GGATG GGATG(9/13)
479 GGATG
Ksp632I 244 GAAGAG CTCTTC(1/4)
531 GAAGAG
KspI 62 CCGCGG CCGC^GG
116 CCGCGG
MflI 327 GGATCT [GA]^GATC[CT]
643 GGATCT
RsaI 216 GTAC GT^AC
507 GTAC
SacII 62 CCGCGG CCGC^GG
116 CCGCGG
TaqI 568 TCGA T^CGA
596 TCGA
Tsp509I 128 AATT ^AATT
737 AATT
TthHB8I 568 TCGA T^CGA
596 TCGA
XhoII 327 GGATCT [GA]^GATC[CT]
643 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcyI AlwI AvaI
BanI BbeI BbvI BcnI BfaI
BsaHI BsaJI BsiYI BslI BsoBI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII DsaI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
MaeI MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI SfoI Tru9I TseI