Restriction map for
pFN21AE1061
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HapII Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MseI
MspI MunI MvnI NaeI NarI
NciI NgoMIV NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI Sse8387I SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 189 GCGG CCGC(-3/-1)
AclI 462 AACGTT AA^CGTT
AfaI 827 GTAC GT^AC
AspHI 331 GAGCTC G[AT]GC[AT]^C
AvaII 675 GGTCC G^G[AT]CC
BalI 71 TGGCCA TGG^CCA
BanII 331 GAGCTC G[GA]GC[CT]^C
BbsI 537 GAAGAC GAAGAC(2/6)
BglII 117 AGATCT A^GATCT
BmyI 331 GAGCTC G[AGT]GC[ACT]^C
BpuAI 537 GAAGAC GAAGAC(2/6)
BsiHKAI 331 GAGCTC G[AT]GC[AT]^C
BsmFI 684 GGGAC GGGAC(10/14)
Bsp1286I 331 GAGCTC G[AGT]GC[ACT]^C
BspHI 632 TCATGA T^CATGA
CfoI 701 GCGC GCG^C
DraII 834 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 71 TGGCCA [CT]^GGCC[GA]
EarI 889 CTCTTC CTCTTC(1/4)
EcoO109I 834 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeII 700 GGCGCT [GA]GCGC^[CT]
HgaI 256 GACGC GACGC(5/10)
HhaI 701 GCGC GCG^C
HinP1I 701 GCGC G^CGC
Ksp632I 889 CTCTTC CTCTTC(1/4)
MaeII 463 ACGT A^CGT
MluNI 71 TGGCCA TGG^CCA
MscI 71 TGGCCA TGG^CCA
MspA1I 853 CAGCTG C[AC]G^C[GT]G
NcoI 34 CCATGG C^CATGG
NdeI 472 CATATG CA^TATG
Psp1406I 462 AACGTT AA^CGTT
PvuII 853 CAGCTG CAG^CTG
RcaI 632 TCATGA T^CATGA
RsaI 827 GTAC GT^AC
SacI 331 GAGCTC GAGCT^C
SpeI 151 ACTAGT A^CTAGT
StuI 18 AGGCCT AGG^CCT
Tsp45I 661 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 357 GAATTT [GA]^AATT[CT]
558 AAATTC
AlwNI 643 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
803 CAGCACCTG
ApoI 357 GAATTT [GA]^AATT[CT]
558 AAATTC
BbvCI 785 CCTCAGC CCTCAGC(-5/-2)
850 CCTCAGC
BpmI 10 CTGGAG CTGGAG(16/14)
704 CTGGAG
BseRI 200 CTCCTC GAGGAG(10/8)
783 CTCCTC
BspDI 530 ATCGAT AT^CGAT
745 ATCGAT
BsrI 9 ACTGG ACTGG(1/-1)
777 ACTGG
BssKI 387 CCTGG ^CC[ACGT]GG
501 CCAGG
BstNI 387 CCTGG CC^[AT]GG
501 CCAGG
BstXI 34 CCATGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
501 CCAGGAGAGTGG
Cfr13I 675 GGTCC G^G[ACGT]CC
835 GGCCC
ClaI 530 ATCGAT AT^CGAT
745 ATCGAT
DsaI 34 CCATGG C^C[GA][CT]GG
821 CCACGG
EcoRII 387 CCTGG ^CC[AT]GG
501 CCAGG
MaeIII 465 GTTAC ^GT[ACGT]AC
661 GTCAC
MvaI 387 CCTGG CC^[AT]GG
501 CCAGG
NheI 372 GCTAGC G^CTAGC
456 GCTAGC
NspI 368 GCATGC [GA]CATG^[CT]
601 GCATGC
PleI 426 GAGTC GAGTC(4/5)
817 GAGTC
Sau96I 675 GGTCC G^G[ACGT]CC
835 GGCCC
ScrFI 387 CCTGG CC^[ACGT]GG
501 CCAGG
SphI 368 GCATGC GCATG^C
601 GCATGC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI BbvI BfaI BsaI
BsaJI BsiYI BslI BsmAI BstYI
Cac8I DdeI DpnII EcoT14I Fnu4HI
HaeIII HinfI HphI ItaI MaeI
MboI MboII MflI MnlI MslI
MwoI NdeII NlaIII PstI Sau3AI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XhoII