Restriction map for
pFN21AE3434
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BbeI BbrPI BclI
BfrI BlnI BlpI Bpu1102I BsaHI
BsiWI BsmBI BsmI BspDI BspHI
BsrDI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI Eam1105I
Eco47III Eco81I EcoRV FbaI FseI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI Tru9I XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 52 GGGCCC GGGCC^C
Asp700I 1215 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 280 GGCACC G^G[CT][GA]CC
BciVI 125 GTATCC GTATCC(6/5)
BglII 2000 AGATCT A^GATCT
BsaAI 1336 CACGTA [CT]AC^GT[GA]
BssSI 391 CTCGTG CACGAG(-5/-1)
DraIII 1417 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 243 GACCCGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2528 CGGCCG C^GGCCG
EclXI 2528 CGGCCG C^GGCCG
Eco52I 2528 CGGCCG C^GGCCG
EcoNI 20 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2401 GAATTC G^AATTC
EcoT22I 1024 ATGCAT ATGCA^T
NsiI 1024 ATGCAT ATGCA^T
PaeR7I 1504 CTCGAG C^TCGAG
PshAI 715 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 842 ACCAGGT A^CC[AT]GGT
SfiI 2503 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1504 CTCGAG C^T[CT][GA]AG
SphI 2493 GCATGC GCATG^C
XhoI 1504 CTCGAG C^TCGAG
XmnI 1215 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1219 AAATTC [GA]^AATT[CT]
2401 GAATTC
ApoI 1219 AAATTC [GA]^AATT[CT]
2401 GAATTC
BbvCI 1572 CCTCAGC CCTCAGC(-5/-2)
1773 CCTCAGC
BcgI 314 GCAGGCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
744 GCAGACCTATCG
BfaI 1360 CTAG C^TAG
1717 CTAG
BsaBI 1420 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1605 GATGGTCATC
Bsp1407I 1184 TGTACA T^GTACA
1484 TGTACA
BspLU11I 464 ACATGT A^CATGT
1634 ACATGT
BsrGI 1184 TGTACA T^GTACA
1484 TGTACA
BssHII 159 GCGCGC G^CGCGC
198 GCGCGC
BstEII 498 GGTCACC G^GT[ACGT]ACC
1849 GGTGACC
BstPI 498 GGTCACC G^GT[ACGT]ACC
1849 GGTGACC
BstXI 270 CCATGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1787 CCAAGCCGCTGG
BstYI 896 AGATCC [GA]^GATC[CT]
2000 AGATCT
EcoO65I 498 GGTCACC G^GT[ACGT]ACC
1849 GGTGACC
MaeI 1360 CTAG C^TAG
1717 CTAG
MamI 1420 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1605 GATGGTCATC
MflI 896 AGATCC [GA]^GATC[CT]
2000 AGATCT
PstI 1324 CTGCAG CTGCA^G
1540 CTGCAG
PvuII 730 CAGCTG CAG^CTG
1313 CAGCTG
SacI 805 GAGCTC GAGCT^C
1087 GAGCTC
SapI 1084 GAAGAGC GCTCTTC(1/4)
2443 GCTCTTC
SspBI 1184 TGTACA T^GTACA
1484 TGTACA
TfiI 1386 GATTC G^A[AT]TC
1509 GAATC
XhoII 896 AGATCC [GA]^GATC[CT]
2000 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AccIII AciI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
AspHI AspI AvaI AvaII AviII
BalI BanII BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XmaCI XmaI