Restriction map for
pFN21AE3055
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BanI BanII BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MroI MslI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 158 GTCTAC GT^[AC][GT]AC
AcsI 322 AAATTC [GA]^AATT[CT]
AlwNI 480 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 322 AAATTC [GA]^AATT[CT]
AvaI 618 CCCGAG C^[CT]CG[GA]G
BalI 233 TGGCCA TGG^CCA
BamHI 449 GGATCC G^GATCC
BbsI 569 GAAGAC GAAGAC(2/6)
BbvCI 205 CCTCAGC CCTCAGC(-5/-2)
BbvI 36 GCTGC GCAGC(8/12)
BglII 260 AGATCT A^GATCT
BpuAI 569 GAAGAC GAAGAC(2/6)
BsmAI 542 GTCTC GTCTC(1/5)
BsmFI 372 GGGAC GGGAC(10/14)
BsoBI 618 CCCGAG C^[CT]CG[GA]G
BsrI 375 ACTGG ACTGG(1/-1)
BstF5I 294 GGATG GGATG(2/0)
Cac8I 39 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 494 GGGCC G^G[ACGT]CC
DraI 114 TTTAAA TTT^AAA
EaeI 233 TGGCCA [CT]^GGCC[GA]
EarI 271 CTCTTC CTCTTC(1/4)
Fnu4HI 36 GCTGC GC^[ACGT]GC
FokI 294 GGATG GGATG(9/13)
ItaI 36 GCTGC GC^[ACGT]GC
Ksp632I 271 CTCTTC CTCTTC(1/4)
MluNI 233 TGGCCA TGG^CCA
MscI 233 TGGCCA TGG^CCA
NlaIV 449 GGATCC GG[ACGT]^[ACGT]CC
PleI 201 GAGTC GAGTC(4/5)
Sau96I 494 GGGCC G^G[ACGT]CC
SfaNI 249 GATGC GCATC(5/9)
SspI 283 AATATT AAT^ATT
TfiI 102 GAATC G^A[AT]TC
TseI 36 GCTGC G^C[AT]GC
TspRI 154 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 63 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 450 GATCC GGATC(4/5)
449 GGATC
BsaJI 491 CCTGGG C^C[ACGT][ACGT]GG
619 CCGAGG
BsiYI 270 CCTCTTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
485 CCTGTGCCTGG
BslI 270 CCTCTTCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
485 CCTGTGCCTGG
BstYI 260 AGATCT [GA]^GATC[CT]
449 GGATCC
DpnII 261 GATC ^GATC
450 GATC
HinfI 102 GAATC G^A[ACGT]TC
201 GAGTC
MboI 261 GATC ^GATC
450 GATC
MflI 260 AGATCT [GA]^GATC[CT]
449 GGATCC
MseI 115 TTAA T^TAA
431 TTAA
NdeII 261 GATC ^GATC
450 GATC
Sau3AI 261 GATC ^GATC
450 GATC
SfcI 477 CTACAG C^T[GA][CT]AG
602 CTACAG
Tru9I 115 TTAA T^TAA
431 TTAA
Tsp509I 147 AATT ^AATT
323 AATT
XhoII 260 AGATCT [GA]^GATC[CT]
449 GGATCC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AfaI AluI BfaI BssKI BstNI
DdeI EcoRII HaeIII MaeI MboII
MnlI MvaI NlaIII RsaI ScrFI