Restriction map for
pFN21AE9676
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NciI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 78 GGCGCC G[GA]^CG[CT]C
AlwNI 361 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 598 GGGCCC GGGCC^C
AvaI 98 CCCGAG C^[CT]CG[GA]G
AvaII 69 GGACC G^G[AT]CC
BamHI 620 GGATCC G^GATCC
BanI 78 GGCGCC G^G[CT][GA]CC
BbeI 78 GGCGCC GGCGC^C
BciVI 668 GGATAC GTATCC(6/5)
BfaI 464 CTAG C^TAG
BpmI 739 CTGGAG CTGGAG(16/14)
BsaHI 78 GGCGCC G[GA]^CG[CT]C
BsgI 848 GTGCAG GTGCAG(16/14)
BsmAI 309 GAGAC GTCTC(1/5)
BsmI 436 GAATGC GAATGC(1/-1)
BsoBI 98 CCCGAG C^[CT]CG[GA]G
BsrI 220 ACTGG ACTGG(1/-1)
BssHII 24 GCGCGC G^CGCGC
BstF5I 145 GGATG GGATG(2/0)
DsaI 614 CCATGG C^C[GA][CT]GG
EaeI 637 CGGCCA [CT]^GGCC[GA]
EcoNI 588 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 614 CCATGG C^C[AT][AT]GG
FokI 145 GGATG GGATG(9/13)
HgaI 4 GACGC GACGC(5/10)
Hin1I 78 GGCGCC G[GA]^CG[CT]C
HphI 716 TCACC GGTGA(8/7)
KasI 78 GGCGCC G^GCGCC
MaeI 464 CTAG C^TAG
MaeII 547 ACGT A^CGT
MseI 448 TTAA T^TAA
NaeI 635 GCCGGC GCC^GGC
NarI 78 GGCGCC GG^CGCC
NcoI 614 CCATGG C^CATGG
NgoMIV 635 GCCGGC G^CCGGC
PleI 101 GAGTC GAGTC(4/5)
PpuMI 68 AGGACCC [GA]G^G[AT]CC[CT]
SfcI 304 CTACAG C^T[GA][CT]AG
SfoI 78 GGCGCC GGC^GCC
SgrAI 74 CACCGGCG C[GA]^CCGG[CT]G
SmlI 559 CTCAAG C^T[CT][GA]AG
StyI 614 CCATGG C^C[AT][AT]GG
TaqI 812 TCGA T^CGA
Tru9I 448 TTAA T^TAA
TspRI 270 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 812 TCGA T^CGA
XbaI 463 TCTAGA T^CTAGA
XcmI 73 CCACCGGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 25 CGCG CG^CG
45 CGCG
BbsI 256 GAAGAC GAAGAC(2/6)
153 GTCTTC
BbvI 626 GCAGC GCAGC(8/12)
817 GCTGC
BglII 162 AGATCT A^GATCT
483 AGATCT
BpuAI 256 GAAGAC GAAGAC(2/6)
153 GTCTTC
BsmFI 96 GTCCC GGGAC(10/14)
724 GTCCC
BsrFI 75 ACCGGC [GA]^CCGG[CT]
635 GCCGGC
BstUI 25 CGCG CG^CG
45 CGCG
Cfr10I 75 ACCGGC [GA]^CCGG[CT]
635 GCCGGC
EarI 88 GAAGAG CTCTTC(1/4)
472 GAAGAG
HaeII 78 GGCGCC [GA]GCGC^[CT]
628 AGCGCC
HapII 76 CCGG C^CGG
636 CCGG
HpaII 76 CCGG C^CGG
636 CCGG
Ksp632I 88 GAAGAG CTCTTC(1/4)
472 GAAGAG
MaeIII 340 GTTAC ^GT[ACGT]AC
403 GTTAC
MslI 270 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
417 CATAATAATG
MspI 76 CCGG C^CGG
636 CCGG
MvnI 25 CGCG CG^CG
45 CGCG
SfaNI 577 GATGC GCATC(5/9)
224 GCATC
TseI 626 GCAGC G^C[AT]GC
817 GCTGC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
MboI MboII MflI MnlI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TfiI Tsp509I
XhoII