Restriction map for
pFN21AB8296
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 962 GAATTC [GA]^AATT[CT]
AlwI 1110 GATCC GGATC(4/5)
AocI 763 CCTAAGG CC^T[ACGT]AGG
ApaI 759 GGGCCC GGGCC^C
ApoI 962 GAATTC [GA]^AATT[CT]
Asp700I 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 684 CCTCAGC CCTCAGC(-5/-2)
BclI 1011 TGATCA T^GATCA
BfaI 967 CTAG C^TAG
BlpI 52 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 52 GCTAAGC GC^T[ACGT]AGC
BsaI 325 GAGACC GGTCTC(1/5)
BsmBI 1085 CGTCTC CGTCTC(1/5)
BsrDI 1168 GCAATG GCAATG(2/0)
BsrFI 357 GCCGGC [GA]^CCGG[CT]
BssHII 30 GCGCGC G^CGCGC
BssSI 691 CTCGTG CACGAG(-5/-1)
BstAPI 975 GCACGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1109 AGATCC [GA]^GATC[CT]
Bsu36I 763 CCTAAGG CC^T[ACGT]AGG
CelII 52 GCTAAGC GC^T[ACGT]AGC
Cfr10I 357 GCCGGC [GA]^CCGG[CT]
DrdI 829 GACAAGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 47 CCGTGG C^C[GA][CT]GG
EarI 913 GAAGAG CTCTTC(1/4)
Eco81I 763 CCTAAGG CC^T[ACGT]AGG
EcoNI 900 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 962 GAATTC G^AATTC
EcoRV 607 GATATC GAT^ATC
FbaI 1011 TGATCA T^GATCA
Ksp632I 913 GAAGAG CTCTTC(1/4)
MaeI 967 CTAG C^TAG
MflI 1109 AGATCC [GA]^GATC[CT]
MslI 1249 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 357 GCCGGC GCC^GGC
NgoMIV 357 GCCGGC G^CCGGC
PleI 1062 GAGTC GAGTC(4/5)
PshAI 870 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1251 CTGCAG CTGCA^G
SapI 913 GAAGAGC GCTCTTC(1/4)
StuI 704 AGGCCT AGG^CCT
XhoII 1109 AGATCC [GA]^GATC[CT]
XmnI 922 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 31 CGCG CG^CG
125 CGCG
AspHI 457 GAGCAC G[AT]GC[AT]^C
1138 GAGCAC
AvaI 36 CCCGAG C^[CT]CG[GA]G
1214 CCCGAG
BbeI 423 GGCGCC GGCGC^C
1223 GGCGCC
BbsI 190 GAAGAC GAAGAC(2/6)
574 GAAGAC
BcnI 58 CCGGG CC^[GC]GG
157 CCCGG
BpmI 1120 CTCCAG CTGGAG(16/14)
1241 CTCCAG
BpuAI 190 GAAGAC GAAGAC(2/6)
574 GAAGAC
BseRI 349 GAGGAG GAGGAG(10/8)
772 GAGGAG
BsiHKAI 457 GAGCAC G[AT]GC[AT]^C
1138 GAGCAC
BsoBI 36 CCCGAG C^[CT]CG[GA]G
1214 CCCGAG
BstF5I 1015 CATCC GGATG(2/0)
1155 GGATG
BstUI 31 CGCG CG^CG
125 CGCG
DpnII 1012 GATC ^GATC
1110 GATC
EaeI 635 CGGCCA [CT]^GGCC[GA]
782 TGGCCG
FauI 19 CCCGC CCCGC(4/6)
126 GCGGG
FokI 1015 CATCC GGATG(9/13)
1155 GGATG
HphI 493 GGTGA GGTGA(8/7)
1188 GGTGA
KasI 423 GGCGCC G^GCGCC
1223 GGCGCC
MaeIII 856 GTGAC ^GT[ACGT]AC
1256 GTGAC
MboI 1012 GATC ^GATC
1110 GATC
MspA1I 602 CAGCGG C[AC]G^C[GT]G
872 CAGCGG
MvnI 31 CGCG CG^CG
125 CGCG
NarI 423 GGCGCC GG^CGCC
1223 GGCGCC
NciI 58 CCGGG CC^[GC]GG
157 CCCGG
NdeII 1012 GATC ^GATC
1110 GATC
PpuMI 14 AGGTCCC [GA]G^G[AT]CC[CT]
60 GGGTCCT
Sau3AI 1012 GATC ^GATC
1110 GATC
SfcI 864 CTATAG C^T[GA][CT]AG
1251 CTGCAG
SfoI 423 GGCGCC GGC^GCC
1223 GGCGCC
SmlI 335 CTCAAG C^T[CT][GA]AG
901 CTCAAG
TaqI 521 TCGA T^CGA
611 TCGA
TfiI 885 GATTC G^A[AT]TC
1029 GATTC
Tsp45I 856 GTGAC ^GT[GC]AC
1256 GTGAC
TthHB8I 521 TCGA T^CGA
611 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BanI BanII BbvI BmyI BsaHI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeII MboII
MnlI MspI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
StyI TseI Tsp509I TspRI