Restriction map for
pFN21AA1588
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbvCI BcgI BciVI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsoBI BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII Bst1107I BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DrdI Eam1105I EarI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT14I FseI
FspI Hin1I HpaI KasI KpnI
Ksp632I KspI MaeI MfeI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 860 TCCGGA T^CCGGA
AflII 1297 CTTAAG C^TTAAG
AflIII 1085 ACGCGT A^C[GA][CT]GT
AlwNI 756 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 1649 GGATCC G^GATCC
BclI 924 TGATCA T^GATCA
BfrI 1297 CTTAAG C^TTAAG
BsaBI 1230 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 860 TCCGGA T^CCGGA
BsiEI 945 CGGCCG CG[GA][CT]^CG
BsiWI 1586 CGTACG C^GTACG
BsmBI 1413 GAGACG CGTCTC(1/5)
Bsp1407I 242 TGTACA T^GTACA
BspEI 860 TCCGGA T^CCGGA
BsrFI 475 GCCGGT [GA]^CCGG[CT]
BsrGI 242 TGTACA T^GTACA
BstBI 514 TTCGAA TT^CGAA
BstYI 1649 GGATCC [GA]^GATC[CT]
Cfr10I 475 GCCGGT [GA]^CCGG[CT]
DsaI 1250 CCGTGG C^C[GA][CT]GG
EaeI 945 CGGCCG [CT]^GGCC[GA]
EagI 945 CGGCCG C^GGCCG
EclXI 945 CGGCCG C^GGCCG
Eco52I 945 CGGCCG C^GGCCG
FbaI 924 TGATCA T^GATCA
HincII 1542 GTCAAC GT[CT]^[GA]AC
HindII 1542 GTCAAC GT[CT]^[GA]AC
MamI 1230 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1649 GGATCC [GA]^GATC[CT]
MluI 1085 ACGCGT A^CGCGT
MroI 860 TCCGGA T^CCGGA
MspA1I 1113 CCGCTG C[AC]G^C[GT]G
NspV 514 TTCGAA TT^CGAA
PshAI 835 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 837 CTGCAG CTGCA^G
SfuI 514 TTCGAA TT^CGAA
SmlI 1297 CTTAAG C^T[CT][GA]AG
SspBI 242 TGTACA T^GTACA
TaqI 515 TCGA T^CGA
TthHB8I 515 TCGA T^CGA
XhoII 1649 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 64 GAATTT [GA]^AATT[CT]
102 GAATTT
Alw44I 1089 GTGCAC G^TGCAC
1639 GTGCAC
AlwI 1650 GATCC GGATC(4/5)
1649 GGATC
AocI 792 CCTTAGG CC^T[ACGT]AGG
1716 CCTCAGG
ApaLI 1089 GTGCAC G^TGCAC
1639 GTGCAC
ApoI 64 GAATTT [GA]^AATT[CT]
102 GAATTT
BbrPI 1054 CACGTG CAC^GTG
1726 CACGTG
BsaAI 1054 CACGTG [CT]AC^GT[GA]
1726 CACGTG
BsaWI 860 TCCGGA [AT]^CCGG[AT]
985 ACCGGA
BssSI 696 CACGAG CACGAG(-5/-1)
1204 CTCGTG
BstAPI 755 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1091 GCACGCAGTGC
Bsu36I 792 CCTTAGG CC^T[ACGT]AGG
1716 CCTCAGG
DraII 574 AGGCCCT [GA]G^G[ACGT]CC[CT]
994 AGGCCCT
Eco81I 792 CCTTAGG CC^T[ACGT]AGG
1716 CCTCAGG
EcoO109I 574 AGGCCCT [GA]G^G[ACGT]CC[CT]
994 AGGCCCT
EcoT22I 661 ATGCAT ATGCA^T
854 ATGCAT
HaeII 361 GGCGCT [GA]GCGC^[CT]
373 GGCGCT
HindIII 1022 AAGCTT A^AGCTT
1358 AAGCTT
MseI 1130 TTAA T^TAA
1298 TTAA
NsiI 661 ATGCAT ATGCA^T
854 ATGCAT
PflMI 99 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1149 CCACATGGTGG
PmaCI 1054 CACGTG CAC^GTG
1726 CACGTG
PmlI 1054 CACGTG CAC^GTG
1726 CACGTG
SfcI 267 CTACAG C^T[GA][CT]AG
837 CTGCAG
Tru9I 1130 TTAA T^TAA
1298 TTAA
Van91I 99 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1149 CCACATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AfaI AluI Asp700I
AspHI AvaII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraIII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI StuI TfiI TseI
Tsp45I Tsp509I TspRI XmnI