Restriction map for
pFN21AE1131
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tru9I Tsp509I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflIII 122 ACATGT A^C[GA][CT]GT
AlwI 744 GATCC GGATC(4/5)
BalI 254 TGGCCA TGG^CCA
BanI 89 GGCGCC G^G[CT][GA]CC
BanII 110 GAGCCC G[GA]GC[CT]^C
BbeI 89 GGCGCC GGCGC^C
BbsI 645 GAAGAC GAAGAC(2/6)
BclI 170 TGATCA T^GATCA
BlpI 466 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC
BpuAI 645 GAAGAC GAAGAC(2/6)
BsaAI 103 TACGTG [CT]AC^GT[GA]
BspLU11I 122 ACATGT A^CATGT
BsrFI 156 ACCGGC [GA]^CCGG[CT]
BstBI 755 TTCGAA TT^CGAA
BstF5I 209 GGATG GGATG(2/0)
BstXI 554 CCAATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 466 GCTGAGC GC^T[ACGT]AGC
Cfr10I 156 ACCGGC [GA]^CCGG[CT]
FbaI 170 TGATCA T^GATCA
FokI 209 GGATG GGATG(9/13)
HaeII 89 GGCGCC [GA]GCGC^[CT]
HincII 688 GTCAAC GT[CT]^[GA]AC
HindII 688 GTCAAC GT[CT]^[GA]AC
HphI 168 GGTGA GGTGA(8/7)
KasI 89 GGCGCC G^GCGCC
MluNI 254 TGGCCA TGG^CCA
MscI 254 TGGCCA TGG^CCA
MslI 735 CATTAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 89 GGCGCC GG^CGCC
NspI 122 ACATGT [GA]CATG^[CT]
NspV 755 TTCGAA TT^CGAA
PvuII 464 CAGCTG CAG^CTG
SexAI 26 ACCTGGT A^CC[AT]GGT
SfoI 89 GGCGCC GGC^GCC
SfuI 755 TTCGAA TT^CGAA
SmlI 547 CTCAAG C^T[CT][GA]AG
SspI 179 AATATT AAT^ATT
TfiI 758 GAATC G^A[AT]TC
XcmI 257 CCAACCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 147 CGCG CG^CG
344 CGCG
AcyI 6 GGCGTC G[GA]^CG[CT]C
89 GGCGCC
AfaI 102 GTAC GT^AC
348 GTAC
BcnI 78 CCCGG CC^[GC]GG
539 CCCGG
BmyI 110 GAGCCC G[AGT]GC[ACT]^C
307 GTGCCC
BsaHI 6 GGCGTC G[GA]^CG[CT]C
89 GGCGCC
BsaJI 273 CCCTGG C^C[ACGT][ACGT]GG
560 CCCTGG
BseRI 234 CTCCTC GAGGAG(10/8)
237 CTCCTC
BsmFI 74 GGGAC GGGAC(10/14)
435 GTCCC
Bsp1286I 110 GAGCCC G[AGT]GC[ACT]^C
307 GTGCCC
BstUI 147 CGCG CG^CG
344 CGCG
CfoI 90 GCGC GCG^C
343 GCGC
DpnII 171 GATC ^GATC
744 GATC
FauI 44 CCCGC CCCGC(4/6)
310 CCCGC
HgaI 7 GCGTC GACGC(5/10)
660 GCGTC
HhaI 90 GCGC GCG^C
343 GCGC
Hin1I 6 GGCGTC G[GA]^CG[CT]C
89 GGCGCC
HinP1I 90 GCGC G^CGC
343 GCGC
MaeII 104 ACGT A^CGT
407 ACGT
MaeIII 336 GTCAC ^GT[ACGT]AC
603 GTCAC
MboI 171 GATC ^GATC
744 GATC
MvnI 147 CGCG CG^CG
344 CGCG
NciI 78 CCCGG CC^[GC]GG
539 CCCGG
NdeII 171 GATC ^GATC
744 GATC
PflMI 261 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
627 CCAGAACCTGG
PleI 385 GACTC GAGTC(4/5)
652 GAGTC
PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT]
433 GGGTCCC
RsaI 102 GTAC GT^AC
348 GTAC
Sau3AI 171 GATC ^GATC
744 GATC
TaqI 10 TCGA T^CGA
756 TCGA
Tsp45I 336 GTCAC ^GT[GC]AC
603 GTCAC
TthHB8I 10 TCGA T^CGA
756 TCGA
Van91I 261 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
627 CCAGAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwNI AspI AvaII
BbvI BfaI BpmI BsiYI BslI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DraII EaeI EarI EcoO109I
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI Ksp632I MaeI MboII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV PflFI PstI Sau96I
ScrFI SfaNI SfcI TseI TspRI
Tth111I