Restriction map for
pFN21AE4507
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoRV
FauI FseI FspI HaeII HapII
HgaI HpaI HpaII KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 15 GACGTC GACGT^C
AciI 1192 GCGG CCGC(-3/-1)
AcyI 15 GACGTC G[GA]^CG[CT]C
AflIII 947 ACATGT A^C[GA][CT]GT
AocI 868 CCTAAGG CC^T[ACGT]AGG
AseI 1300 ATTAAT AT^TAAT
AsnI 1300 ATTAAT AT^TAAT
AspHI 894 GAGCTC G[AT]GC[AT]^C
BanII 894 GAGCTC G[GA]GC[CT]^C
BglI 1498 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 894 GAGCTC G[AGT]GC[ACT]^C
BpmI 899 CTGGAG CTGGAG(16/14)
BsaBI 1515 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 15 GACGTC G[GA]^CG[CT]C
BsgI 889 GTGCAG GTGCAG(16/14)
BsiHKAI 894 GAGCTC G[AT]GC[AT]^C
BsmFI 698 GGGAC GGGAC(10/14)
Bsp1286I 894 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 947 ACATGT A^CATGT
BsrDI 1209 CATTGC GCAATG(2/0)
BstBI 776 TTCGAA TT^CGAA
BstEII 1070 GGTTACC G^GT[ACGT]ACC
BstPI 1070 GGTTACC G^GT[ACGT]ACC
Bsu36I 868 CCTAAGG CC^T[ACGT]AGG
CfoI 1407 GCGC GCG^C
DsaI 1158 CCATGG C^C[GA][CT]GG
Eco81I 868 CCTAAGG CC^T[ACGT]AGG
EcoNI 864 CCTGCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1070 GGTTACC G^GT[ACGT]ACC
EcoRI 1343 GAATTC G^AATTC
EcoT22I 1283 ATGCAT ATGCA^T
HhaI 1407 GCGC GCG^C
Hin1I 15 GACGTC G[GA]^CG[CT]C
HinP1I 1407 GCGC G^CGC
HincII 165 GTTGAC GT[CT]^[GA]AC
HindII 165 GTTGAC GT[CT]^[GA]AC
MaeII 16 ACGT A^CGT
MamI 1515 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1158 CCATGG C^CATGG
NdeI 649 CATATG CA^TATG
NsiI 1283 ATGCAT ATGCA^T
NspI 947 ACATGT [GA]CATG^[CT]
NspV 776 TTCGAA TT^CGAA
PflMI 1467 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 15 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1300 ATTAAT AT^TAAT
SacI 894 GAGCTC GAGCT^C
SapI 130 GAAGAGC GCTCTTC(1/4)
SfcI 366 CTACAG C^T[GA][CT]AG
SfuI 776 TTCGAA TT^CGAA
SspI 356 AATATT AAT^ATT
Tsp45I 157 GTGAC ^GT[GC]AC
Van91I 1467 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspI 1257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACCCTGTC
AvaII 699 GGACC G^G[AT]CC
1256 GGACC
BamHI 1163 GGATCC G^GATCC
1457 GGATCC
BbvI 1234 GCAGC GCAGC(8/12)
1438 GCAGC
BclI 609 TGATCA T^GATCA
938 TGATCA
BfaI 79 CTAG C^TAG
721 CTAG
BsmAI 745 GAGAC GTCTC(1/5)
3 GTCTC
BsmI 1178 GCATTC GAATGC(1/-1)
1486 GCATTC
Bsp1407I 509 TGTACA T^GTACA
1061 TGTACA
BsrGI 509 TGTACA T^GTACA
1061 TGTACA
BstF5I 1096 CATCC GGATG(2/0)
1476 GGATG
EcoT14I 1158 CCATGG C^C[AT][AT]GG
1226 CCAAGG
FbaI 609 TGATCA T^GATCA
938 TGATCA
Fnu4HI 1234 GCAGC GC^[ACGT]GC
1438 GCAGC
FokI 1096 CATCC GGATG(9/13)
1476 GGATG
HaeIII 86 GGCC GG^CC
1497 GGCC
HindIII 1109 AAGCTT A^AGCTT
1555 AAGCTT
ItaI 1234 GCAGC GC^[ACGT]GC
1438 GCAGC
MaeI 79 CTAG C^TAG
721 CTAG
PflFI 1257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACCCTGTC
PleI 427 GAGTC GAGTC(4/5)
1433 GAGTC
SspBI 509 TGTACA T^GTACA
1061 TGTACA
StyI 1158 CCATGG C^C[AT][AT]GG
1226 CCAAGG
TfiI 262 GATTC G^A[AT]TC
521 GATTC
TseI 1234 GCAGC G^C[AT]GC
1438 GCAGC
TspRI 844 CACTG CA[GC]TG[ACGT][ACGT]^
1242 CAGTG
Tth111I 1257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BpuAI BsaJI BsiYI BslI
BspHI BsrI BssKI BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII HinfI HphI Ksp632I MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI Tru9I Tsp509I TthHB8I
XhoII