Restriction map for
pFN21AE6449
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssSI Bst1107I
BstBI BstF5I BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV FokI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MluI MroI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1451 GGTACC G^GTACC
AlwNI 114 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 1411 GGCGCGCC GG^CGCGCC
AseI 883 ATTAAT AT^TAAT
AsnI 883 ATTAAT AT^TAAT
Asp718I 1451 GGTACC G^GTACC
BbsI 820 GTCTTC GAAGAC(2/6)
BcnI 1353 CCCGG CC^[GC]GG
BlpI 123 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTCAGC GC^T[ACGT]AGC
BpuAI 820 GTCTTC GAAGAC(2/6)
BsaI 933 GGTCTC GGTCTC(1/5)
BsaWI 1544 ACCGGA [AT]^CCGG[AT]
BseRI 55 CTCCTC GAGGAG(10/8)
Bsp1407I 200 TGTACA T^GTACA
BsrDI 1151 GCAATG GCAATG(2/0)
BsrGI 200 TGTACA T^GTACA
BssHII 1412 GCGCGC G^CGCGC
BstAPI 694 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1087 GGTCACC G^GT[ACGT]ACC
BstPI 1087 GGTCACC G^GT[ACGT]ACC
BstYI 738 GGATCT [GA]^GATC[CT]
CelII 123 GCTCAGC GC^T[ACGT]AGC
DraI 1522 TTTAAA TTT^AAA
EarI 211 GAAGAG CTCTTC(1/4)
EcoNI 1338 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1087 GGTCACC G^GT[ACGT]ACC
EcoT22I 752 ATGCAT ATGCA^T
KpnI 1451 GGTACC GGTAC^C
Ksp632I 211 GAAGAG CTCTTC(1/4)
MfeI 965 CAATTG C^AATTG
MflI 738 GGATCT [GA]^GATC[CT]
MunI 965 CAATTG C^AATTG
NciI 1353 CCCGG CC^[GC]GG
NdeI 1146 CATATG CA^TATG
NsiI 752 ATGCAT ATGCA^T
PleI 11 GACTC GAGTC(4/5)
PpuMI 146 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 883 ATTAAT AT^TAAT
PvuII 31 CAGCTG CAG^CTG
SmlI 516 CTTGAG C^T[CT][GA]AG
SspBI 200 TGTACA T^GTACA
StuI 137 AGGCCT AGG^CCT
XbaI 331 TCTAGA T^CTAGA
XhoII 738 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 471 CGCG CG^CG
1413 CGCG
AlwI 738 GGATC GGATC(4/5)
925 GGATC
Asp700I 216 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
310 GAACATCTTC
BalI 2 TGGCCA TGG^CCA
35 TGGCCA
BfaI 332 CTAG C^TAG
584 CTAG
BmyI 100 GTGCCC G[AGT]GC[ACT]^C
549 GGGCAC
BsmAI 1216 GAGAC GTCTC(1/5)
934 GTCTC
BsmFI 146 GGGAC GGGAC(10/14)
776 GGGAC
Bsp1286I 100 GTGCCC G[AGT]GC[ACT]^C
549 GGGCAC
BspMI 419 ACCTGC ACCTGC(4/8)
1421 ACCTGC
BstUI 471 CGCG CG^CG
1413 CGCG
DraII 146 GGGACCT [GA]G^G[ACGT]CC[CT]
404 AGGCCCT
DraIII 166 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
552 CACACAGTG
DsaI 1128 CCATGG C^C[GA][CT]GG
1163 CCATGG
EcoO109I 146 GGGACCT [GA]G^G[ACGT]CC[CT]
404 AGGCCCT
HapII 1354 CCGG C^CGG
1545 CCGG
HpaII 1354 CCGG C^CGG
1545 CCGG
MaeI 332 CTAG C^TAG
584 CTAG
MaeII 235 ACGT A^CGT
1472 ACGT
MluNI 2 TGGCCA TGG^CCA
35 TGGCCA
MscI 2 TGGCCA TGG^CCA
35 TGGCCA
MspA1I 31 CAGCTG C[AC]G^C[GT]G
1363 CCGCTG
MspI 1354 CCGG C^CGG
1545 CCGG
MvnI 471 CGCG CG^CG
1413 CGCG
NcoI 1128 CCATGG C^CATGG
1163 CCATGG
SexAI 167 ACCAGGT A^CC[AT]GGT
1447 ACCTGGT
SphI 128 GCATGC GCATG^C
423 GCATGC
TaqI 496 TCGA T^CGA
1030 TCGA
TfiI 498 GATTC G^A[AT]TC
1032 GAATC
TthHB8I 496 TCGA T^CGA
1030 TCGA
XmnI 216 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
310 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanI BbvI BclI BpmI
BsaJI BsiYI BslI BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
FbaI Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I