Restriction map for
pFN21AA0045
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccII AcyI AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AviII AvrII BbeI
BcgI BlnI BsaHI BsaI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI Bsu36I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HaeII Hin1I KasI KpnI
KspI MluI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmeI PvuI
RsrII SacII SbfI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AclI 3305 AACGTT AA^CGTT
AflII 5887 CTTAAG C^TTAAG
AhdI 2552 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 65 GGGCCC GGGCC^C
AseI 5106 ATTAAT AT^TAAT
AsnI 5106 ATTAAT AT^TAAT
AspEI 2552 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5260 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 5111 TGGCCA TGG^CCA
BbrPI 2378 CACGTG CAC^GTG
BciVI 3237 GGATAC GTATCC(6/5)
BclI 5655 TGATCA T^GATCA
BfrI 5887 CTTAAG C^TTAAG
BglI 3027 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 943 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 943 GCTGAGC GC^T[ACGT]AGC
BsaAI 2378 CACGTG [CT]AC^GT[GA]
BsmI 5641 GAATGC GAATGC(1/-1)
BsrFI 8 ACCGGC [GA]^CCGG[CT]
Bst1107I 4100 GTATAC GTA^TAC
BstZ17I 4100 GTATAC GTA^TAC
CelII 943 GCTGAGC GC^T[ACGT]AGC
Cfr10I 8 ACCGGC [GA]^CCGG[CT]
DraI 3771 TTTAAA TTT^AAA
DraIII 650 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5111 TGGCCA [CT]^GGCC[GA]
Eam1105I 2552 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1711 GAATTC G^AATTC
FbaI 5655 TGATCA T^GATCA
HgaI 709 GCGTC GACGC(5/10)
HpaI 4521 GTTAAC GTT^AAC
MluNI 5111 TGGCCA TGG^CCA
MscI 5111 TGGCCA TGG^CCA
MslI 4068 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 2858 CCATGG C^CATGG
PflFI 5260 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3589 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2378 CACGTG CAC^GTG
PmlI 2378 CACGTG CAC^GTG
PshBI 5106 ATTAAT AT^TAAT
Psp1406I 3305 AACGTT AA^CGTT
SacI 946 GAGCTC GAGCT^C
SalI 1052 GTCGAC G^TCGAC
SexAI 651 ACCAGGT A^CC[AT]GGT
SfiI 3026 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 5260 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3589 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccIII 2481 TCCGGA T^CCGGA
5786 TCCGGA
AvaI 206 CTCGAG C^[CT]CG[GA]G
2456 CTCGAG
BsaBI 2849 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5531 GATTTGAATC
BsaWI 2481 TCCGGA [AT]^CCGG[AT]
5786 TCCGGA
BseAI 2481 TCCGGA T^CCGGA
5786 TCCGGA
BsgI 371 CTGCAC GTGCAG(16/14)
500 GTGCAG
BsoBI 206 CTCGAG C^[CT]CG[GA]G
2456 CTCGAG
BspEI 2481 TCCGGA T^CCGGA
5786 TCCGGA
BspHI 4565 TCATGA T^CATGA
5670 TCATGA
DsaI 983 CCACGG C^C[GA][CT]GG
2858 CCATGG
EcoNI 5039 CCTTTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5773 CCTGTTGGAGG
FauI 1678 GCGGG CCCGC(4/6)
3202 GCGGG
HindIII 1208 AAGCTT A^AGCTT
3129 AAGCTT
MamI 2849 GATCGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5531 GATTTGAATC
MroI 2481 TCCGGA T^CCGGA
5786 TCCGGA
PaeR7I 206 CTCGAG C^TCGAG
2456 CTCGAG
PpuMI 2550 AGGACCT [GA]G^G[AT]CC[CT]
4820 AGGACCT
PshAI 4936 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5808 GACAATTGTC
RcaI 4565 TCATGA T^CATGA
5670 TCATGA
ScaI 3041 AGTACT AGT^ACT
5564 AGTACT
SpeI 2422 ACTAGT A^CTAGT
4498 ACTAGT
StuI 1622 AGGCCT AGG^CCT
5650 AGGCCT
XhoI 206 CTCGAG C^TCGAG
2456 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MspA1I MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI