Restriction map for
pFN21AA0898
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BglI BlnI BlpI
Bpu1102I BsaAI BsaI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 455 GGCGTC G[GA]^CG[CT]C
AflII 1873 CTTAAG C^TTAAG
AflIII 414 ACACGT A^C[GA][CT]GT
Alw44I 350 GTGCAC G^TGCAC
ApaLI 350 GTGCAC G^TGCAC
AseI 1848 ATTAAT AT^TAAT
AsnI 1848 ATTAAT AT^TAAT
AspI 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 28 GCTGAGG CCTCAGC(-5/-2)
BcgI 747 GCAAGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1162 GGATAC GTATCC(6/5)
BclI 470 TGATCA T^GATCA
BfrI 1873 CTTAAG C^TTAAG
BsaHI 455 GGCGTC G[GA]^CG[CT]C
BsaWI 1880 ACCGGA [AT]^CCGG[AT]
BsgI 1271 CTGCAC GTGCAG(16/14)
BsiYI 2686 CCTGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 2686 CCTGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 457 CGTCTC CGTCTC(1/5)
BsmFI 1238 GGGAC GGGAC(10/14)
BspHI 2789 TCATGA T^CATGA
BsrFI 2480 ACCGGC [GA]^CCGG[CT]
BstBI 2321 TTCGAA TT^CGAA
BstEII 2565 GGTCACC G^GT[ACGT]ACC
BstPI 2565 GGTCACC G^GT[ACGT]ACC
CfoI 123 GCGC GCG^C
Cfr10I 2480 ACCGGC [GA]^CCGG[CT]
EaeI 2045 TGGCCG [CT]^GGCC[GA]
EcoNI 2686 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2565 GGTCACC G^GT[ACGT]ACC
EcoRV 711 GATATC GAT^ATC
EcoT22I 367 ATGCAT ATGCA^T
FbaI 470 TGATCA T^GATCA
HaeII 122 GGCGCT [GA]GCGC^[CT]
HhaI 123 GCGC GCG^C
Hin1I 455 GGCGTC G[GA]^CG[CT]C
HinP1I 123 GCGC G^CGC
MfeI 2238 CAATTG C^AATTG
MunI 2238 CAATTG C^AATTG
NsiI 367 ATGCAT ATGCA^T
NspV 2321 TTCGAA TT^CGAA
PflFI 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1743 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1848 ATTAAT AT^TAAT
RcaI 2789 TCATGA T^CATGA
SfuI 2321 TTCGAA TT^CGAA
SmaI 1235 CCCGGG CCC^GGG
SmlI 1873 CTTAAG C^T[CT][GA]AG
SphI 1426 GCATGC GCATG^C
Tth111I 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1235 CCCGGG C^CCGGG
XmaI 1235 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AvaI 1235 CCCGGG C^[CT]CG[GA]G
2429 CCCGAG
BbsI 621 GAAGAC GAAGAC(2/6)
2476 GAAGAC
BglII 1548 AGATCT A^GATCT
2856 AGATCT
BpuAI 621 GAAGAC GAAGAC(2/6)
2476 GAAGAC
BseRI 1501 GAGGAG GAGGAG(10/8)
2737 GAGGAG
BsoBI 1235 CCCGGG C^[CT]CG[GA]G
2429 CCCGAG
BssSI 1409 CACGAG CACGAG(-5/-1)
1531 CACGAG
BstAPI 678 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1732 GCAGCTGCTGC
DraI 385 TTTAAA TTT^AAA
2013 TTTAAA
DraII 1743 AGGACCC [GA]G^G[ACGT]CC[CT]
2847 AGGCCCT
DsaI 764 CCATGG C^C[GA][CT]GG
1761 CCATGG
EcoO109I 1743 AGGACCC [GA]G^G[ACGT]CC[CT]
2847 AGGCCCT
HgaI 456 GCGTC GACGC(5/10)
867 GCGTC
HindIII 598 AAGCTT A^AGCTT
2492 AAGCTT
MaeII 416 ACGT A^CGT
1209 ACGT
NcoI 764 CCATGG C^CATGG
1761 CCATGG
SapI 2324 GAAGAGC GCTCTTC(1/4)
2746 GAAGAGC
SpeI 186 ACTAGT A^CTAGT
1279 ACTAGT
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaBI BsaJI BsiHKAI BsmAI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII