Restriction map for
pFN21AE6128
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsiHKAI BsiWI BsoBI BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Cac8I
CelII CfoI ClaI DraI DraIII
DrdI Eam1105I EarI Eco47III EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI Ksp632I MaeII MaeIII MamI
MfeI MflI MluI MluNI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI TfiI Tru9I Tsp45I TspRI
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 165 TCCGGA T^CCGGA
AfaI 231 GTAC GT^AC
AlwI 279 GATCC GGATC(4/5)
AlwNI 334 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 82 CCTGAGG CC^T[ACGT]AGG
AspI 126 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 45 CCCGG CC^[GC]GG
BpmI 94 CTGGAG CTGGAG(16/14)
BsaI 124 GAGACC GGTCTC(1/5)
BseAI 165 TCCGGA T^CCGGA
BsgI 26 GTGCAG GTGCAG(16/14)
BsiEI 185 CGGCCG CG[GA][CT]^CG
BsmBI 304 GAGACG CGTCTC(1/5)
BsmI 389 GAATGC GAATGC(1/-1)
Bsp1407I 230 TGTACA T^GTACA
BspEI 165 TCCGGA T^CCGGA
BsrFI 22 GCCGGT [GA]^CCGG[CT]
BsrGI 230 TGTACA T^GTACA
BsrI 39 CCAGT ACTGG(1/-1)
BstAPI 333 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 82 CCTGAGG CC^T[ACGT]AGG
Cfr10I 22 GCCGGT [GA]^CCGG[CT]
CpoI 178 CGGTCCG CG^G[AT]CCG
DsaI 182 CCGCGG C^C[GA][CT]GG
EaeI 185 CGGCCG [CT]^GGCC[GA]
EagI 185 CGGCCG C^GGCCG
EclXI 185 CGGCCG C^GGCCG
Eco52I 185 CGGCCG C^GGCCG
Eco81I 82 CCTGAGG CC^T[ACGT]AGG
EcoNI 367 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 70 GACGC GACGC(5/10)
KspI 182 CCGCGG CCGC^GG
MboII 288 TCTTC GAAGA(8/7)
MroI 165 TCCGGA T^CCGGA
NciI 45 CCCGG CC^[GC]GG
PflFI 126 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 363 AGGACCT [GA]G^G[AT]CC[CT]
PstI 340 CTGCAG CTGCA^G
RsaI 231 GTAC GT^AC
RsrII 178 CGGTCCG CG^G[AT]CCG
SacII 182 CCGCGG CCGC^GG
SfcI 340 CTGCAG C^T[GA][CT]AG
SspBI 230 TGTACA T^GTACA
Tsp509I 53 AATT ^AATT
Tth111I 126 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccII 72 CGCG CG^CG
183 CGCG
AluI 92 AGCT AG^CT
116 AGCT
BanII 376 GGGCTC G[GA]GC[CT]^C
398 GGGCTC
BfaI 118 CTAG C^TAG
361 CTAG
BmyI 376 GGGCTC G[AGT]GC[ACT]^C
398 GGGCTC
BsaWI 165 TCCGGA [AT]^CCGG[AT]
176 TCCGGT
BsmFI 262 GGGAC GGGAC(10/14)
42 GTCCC
Bsp1286I 376 GGGCTC G[AGT]GC[ACT]^C
398 GGGCTC
BstUI 72 CGCG CG^CG
183 CGCG
DraII 146 GGGGCCT [GA]G^G[ACGT]CC[CT]
363 AGGACCT
EcoO109I 146 GGGGCCT [GA]G^G[ACGT]CC[CT]
363 AGGACCT
HaeIII 148 GGCC GG^CC
186 GGCC
MaeI 118 CTAG C^TAG
361 CTAG
MspA1I 182 CCGCGG C[AC]G^C[GT]G
346 CAGCGG
MvnI 72 CGCG CG^CG
183 CGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AvaII BbvI BsaJI BseRI
BsiYI BslI BsmAI BssKI BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HapII HpaII ItaI MboI MnlI
MspI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI TseI