Restriction map for
pFN21AB8351
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BciVI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MfeI MluI MroI MseI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tsp45I Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AflIII 716 ACATGT A^C[GA][CT]GT
AlwI 744 GATCC GGATC(4/5)
AlwNI 121 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 423 CCTGAGG CC^T[ACGT]AGG
AspHI 46 GAGCTC G[AT]GC[AT]^C
AvaI 349 CTCGAG C^[CT]CG[GA]G
BalI 191 TGGCCA TGG^CCA
BanII 46 GAGCTC G[GA]GC[CT]^C
BbeI 280 GGCGCC GGCGC^C
BcgI 384 CGACCGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 494 TGATCA T^GATCA
BglI 109 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 353 AGATCT A^GATCT
BsgI 315 CTGCAC GTGCAG(16/14)
BsiHKAI 46 GAGCTC G[AT]GC[AT]^C
BsmAI 259 GAGAC GTCTC(1/5)
BsmBI 259 GAGACG CGTCTC(1/5)
BsoBI 349 CTCGAG C^[CT]CG[GA]G
Bsp1407I 35 TGTACA T^GTACA
BspLU11I 716 ACATGT A^CATGT
BspMI 93 GCAGGT ACCTGC(4/8)
BsrDI 390 CATTGC GCAATG(2/0)
BsrFI 30 GCCGGT [GA]^CCGG[CT]
BsrGI 35 TGTACA T^GTACA
BstYI 353 AGATCT [GA]^GATC[CT]
Bsu36I 423 CCTGAGG CC^T[ACGT]AGG
Cfr10I 30 GCCGGT [GA]^CCGG[CT]
DraII 695 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 659 CGGCCG C^GGCCG
EclXI 659 CGGCCG C^GGCCG
Eco52I 659 CGGCCG C^GGCCG
Eco81I 423 CCTGAGG CC^T[ACGT]AGG
EcoNI 423 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 695 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 494 TGATCA T^GATCA
HaeII 280 GGCGCC [GA]GCGC^[CT]
KasI 280 GGCGCC G^GCGCC
MboII 356 TCTTC GAAGA(8/7)
MflI 353 AGATCT [GA]^GATC[CT]
MluNI 191 TGGCCA TGG^CCA
MscI 191 TGGCCA TGG^CCA
NarI 280 GGCGCC GG^CGCC
NspI 716 ACATGT [GA]CATG^[CT]
PaeR7I 349 CTCGAG C^TCGAG
PflMI 549 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 695 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 6 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 46 GAGCTC GAGCT^C
SfoI 280 GGCGCC GGC^GCC
SmlI 349 CTCGAG C^T[CT][GA]AG
SspBI 35 TGTACA T^GTACA
TfiI 288 GAATC G^A[AT]TC
Tsp509I 199 AATT ^AATT
Van91I 549 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 579 CCAGCAGGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 349 CTCGAG C^TCGAG
XhoII 353 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BseRI 691 GAGGAG GAGGAG(10/8)
814 GAGGAG
BsiEI 384 CGACCG CG[GA][CT]^CG
659 CGGCCG
BsmFI 476 GGGAC GGGAC(10/14)
420 GTCCC
BsrBI 747 CCGCTC CCGCTC(-3/-3)
462 GAGCGG
Cac8I 26 GCGGGC GC[ACGT]^[ACGT]GC
615 GCCCGC
EaeI 191 TGGCCA [CT]^GGCC[GA]
659 CGGCCG
EcoT14I 377 CCATGG C^C[AT][AT]GG
533 CCATGG
HgaI 106 GACGC GACGC(5/10)
261 GACGC
NcoI 377 CCATGG C^CATGG
533 CCATGG
PleI 337 GACTC GAGTC(4/5)
418 GAGTC
PstI 127 CTGCAG CTGCA^G
802 CTGCAG
StyI 377 CCATGG C^C[AT][AT]GG
533 CCATGG
TaqI 350 TCGA T^CGA
359 TCGA
TthHB8I 350 TCGA T^CGA
359 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstUI CfoI
Cfr13I DdeI DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MboI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI TspRI