################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BciVI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp45I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AflIII 716 ACATGT A^C[GA][CT]GT AlwI 744 GATCC GGATC(4/5) AlwNI 121 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 423 CCTGAGG CC^T[ACGT]AGG AspHI 46 GAGCTC G[AT]GC[AT]^C AvaI 349 CTCGAG C^[CT]CG[GA]G BalI 191 TGGCCA TGG^CCA BanII 46 GAGCTC G[GA]GC[CT]^C BbeI 280 GGCGCC GGCGC^C BcgI 384 CGACCGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 494 TGATCA T^GATCA BglI 109 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 353 AGATCT A^GATCT BsgI 315 CTGCAC GTGCAG(16/14) BsiHKAI 46 GAGCTC G[AT]GC[AT]^C BsmAI 259 GAGAC GTCTC(1/5) BsmBI 259 GAGACG CGTCTC(1/5) BsoBI 349 CTCGAG C^[CT]CG[GA]G Bsp1407I 35 TGTACA T^GTACA BspLU11I 716 ACATGT A^CATGT BspMI 93 GCAGGT ACCTGC(4/8) BsrDI 390 CATTGC GCAATG(2/0) BsrFI 30 GCCGGT [GA]^CCGG[CT] BsrGI 35 TGTACA T^GTACA BstYI 353 AGATCT [GA]^GATC[CT] Bsu36I 423 CCTGAGG CC^T[ACGT]AGG Cfr10I 30 GCCGGT [GA]^CCGG[CT] DraII 695 AGGACCT [GA]G^G[ACGT]CC[CT] EagI 659 CGGCCG C^GGCCG EclXI 659 CGGCCG C^GGCCG Eco52I 659 CGGCCG C^GGCCG Eco81I 423 CCTGAGG CC^T[ACGT]AGG EcoNI 423 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 695 AGGACCT [GA]G^G[ACGT]CC[CT] FbaI 494 TGATCA T^GATCA HaeII 280 GGCGCC [GA]GCGC^[CT] KasI 280 GGCGCC G^GCGCC MboII 356 TCTTC GAAGA(8/7) MflI 353 AGATCT [GA]^GATC[CT] MluNI 191 TGGCCA TGG^CCA MscI 191 TGGCCA TGG^CCA NarI 280 GGCGCC GG^CGCC NspI 716 ACATGT [GA]CATG^[CT] PaeR7I 349 CTCGAG C^TCGAG PflMI 549 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 695 AGGACCT [GA]G^G[AT]CC[CT] PshAI 6 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 46 GAGCTC GAGCT^C SfoI 280 GGCGCC GGC^GCC SmlI 349 CTCGAG C^T[CT][GA]AG SspBI 35 TGTACA T^GTACA TfiI 288 GAATC G^A[AT]TC Tsp509I 199 AATT ^AATT Van91I 549 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 579 CCAGCAGGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 349 CTCGAG C^TCGAG XhoII 353 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BseRI 691 GAGGAG GAGGAG(10/8) 814 GAGGAG BsiEI 384 CGACCG CG[GA][CT]^CG 659 CGGCCG BsmFI 476 GGGAC GGGAC(10/14) 420 GTCCC BsrBI 747 CCGCTC CCGCTC(-3/-3) 462 GAGCGG Cac8I 26 GCGGGC GC[ACGT]^[ACGT]GC 615 GCCCGC EaeI 191 TGGCCA [CT]^GGCC[GA] 659 CGGCCG EcoT14I 377 CCATGG C^C[AT][AT]GG 533 CCATGG HgaI 106 GACGC GACGC(5/10) 261 GACGC NcoI 377 CCATGG C^CATGG 533 CCATGG PleI 337 GACTC GAGTC(4/5) 418 GAGTC PstI 127 CTGCAG CTGCA^G 802 CTGCAG StyI 377 CCATGG C^C[AT][AT]GG 533 CCATGG TaqI 350 TCGA T^CGA 359 TCGA TthHB8I 350 TCGA T^CGA 359 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BanI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI CfoI Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MboI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI