Restriction map for
pFN21AB7321
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRV FbaI FseI FspI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 486 GGTACC G^GTACC
Aor51HI 3382 AGCGCT AGC^GCT
Asp718I 486 GGTACC G^GTACC
BamHI 1717 GGATCC G^GATCC
BanI 486 GGTACC G^G[CT][GA]CC
BciVI 3144 GGATAC GTATCC(6/5)
BsaAI 1678 TACGTG [CT]AC^GT[GA]
BsmFI 1683 GGGAC GGGAC(10/14)
Bsp1407I 598 TGTACA T^GTACA
BsrGI 598 TGTACA T^GTACA
Bst1107I 672 GTATAC GTA^TAC
BstZ17I 672 GTATAC GTA^TAC
CfoI 3383 GCGC GCG^C
DraII 425 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 2164 CTCTTC CTCTTC(1/4)
Eco47III 3382 AGCGCT AGC^GCT
EcoO109I 425 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2871 GAATTC G^AATTC
FauI 22 CCCGC CCCGC(4/6)
HaeII 3382 AGCGCT [GA]GCGC^[CT]
HapII 1279 CCGG C^CGG
HgaI 730 GCGTC GACGC(5/10)
HhaI 3383 GCGC GCG^C
HinP1I 3383 GCGC G^CGC
HpaII 1279 CCGG C^CGG
KpnI 486 GGTACC GGTAC^C
Ksp632I 2164 CTCTTC CTCTTC(1/4)
MspI 1279 CCGG C^CGG
NheI 3235 GCTAGC G^CTAGC
PpuMI 425 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 332 CAGCTG CAG^CTG
ScaI 1441 AGTACT AGT^ACT
SmlI 3119 CTCAAG C^T[CT][GA]AG
SspBI 598 TGTACA T^GTACA
StuI 3164 AGGCCT AGG^CCT
TaqI 3220 TCGA T^CGA
TthHB8I 3220 TCGA T^CGA
XbaI 1078 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccI 672 GTATAC GT^[AC][GT]AC
2945 GTAGAC
BanII 19 GAGCCC G[GA]GC[CT]^C
3214 GGGCTC
BsaBI 435 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1837 GATATGGATC
BsgI 1702 GTGCAG GTGCAG(16/14)
2384 GTGCAG
BspMI 2421 ACCTGC ACCTGC(4/8)
3566 GCAGGT
DraI 2620 TTTAAA TTT^AAA
3183 TTTAAA
DraIII 1360 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2644 CACTTTGTG
EcoNI 355 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2759 CCTATGTAAGG
EcoT22I 784 ATGCAT ATGCA^T
3367 ATGCAT
HincII 2787 GTTGAC GT[CT]^[GA]AC
3204 GTCAAC
HindII 2787 GTTGAC GT[CT]^[GA]AC
3204 GTCAAC
MaeII 410 ACGT A^CGT
1679 ACGT
MamI 435 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1837 GATATGGATC
NdeI 1211 CATATG CA^TATG
1471 CATATG
NsiI 784 ATGCAT ATGCA^T
3367 ATGCAT
PstI 335 CTGCAG CTGCA^G
2423 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
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AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BalI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspHI
BspLU11I BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII XmnI