Restriction map for
pFN21AE3289
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KspI MluI MroI MspA1I MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1529 GGTACC G^GTACC
Alw44I 539 GTGCAC G^TGCAC
ApaLI 539 GTGCAC G^TGCAC
Asp718I 1529 GGTACC G^GTACC
AspHI 539 GTGCAC G[AT]GC[AT]^C
AvaII 1857 GGTCC G^G[AT]CC
BalI 289 TGGCCA TGG^CCA
BbsI 1844 GTCTTC GAAGAC(2/6)
BglII 1269 AGATCT A^GATCT
BpmI 1310 CTCCAG CTGGAG(16/14)
BpuAI 1844 GTCTTC GAAGAC(2/6)
BsaWI 308 ACCGGA [AT]^CCGG[AT]
BsgI 113 GTGCAG GTGCAG(16/14)
BsiHKAI 539 GTGCAC G[AT]GC[AT]^C
BspDI 284 ATCGAT AT^CGAT
BsrDI 1286 CATTGC GCAATG(2/0)
BstXI 569 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 284 ATCGAT AT^CGAT
DraI 2220 TTTAAA TTT^AAA
DraII 1856 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 149 GACTTTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 209 CCATGG C^C[GA][CT]GG
EaeI 289 TGGCCA [CT]^GGCC[GA]
EarI 374 CTCTTC CTCTTC(1/4)
EcoO109I 1856 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 592 GATATC GAT^ATC
HapII 309 CCGG C^CGG
HindIII 678 AAGCTT A^AGCTT
HpaII 309 CCGG C^CGG
KpnI 1529 GGTACC GGTAC^C
Ksp632I 374 CTCTTC CTCTTC(1/4)
MfeI 355 CAATTG C^AATTG
MluNI 289 TGGCCA TGG^CCA
MscI 289 TGGCCA TGG^CCA
MspI 309 CCGG C^CGG
MunI 355 CAATTG C^AATTG
NcoI 209 CCATGG C^CATGG
NdeI 317 CATATG CA^TATG
PpuMI 1856 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 373 GCTCTTC GCTCTTC(1/4)
ScaI 2047 AGTACT AGT^ACT
SspI 1398 AATATT AAT^ATT
XbaI 237 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 44 GTAGAC GT^[AC][GT]AC
1108 GTAGAC
AlwI 1246 GATCC GGATC(4/5)
687 GGATC
BanI 1529 GGTACC G^G[CT][GA]CC
1661 GGTGCC
BcgI 169 CGAGTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
332 GCAGAGATGTCG
BclI 1274 TGATCA T^GATCA
1449 TGATCA
BmyI 539 GTGCAC G[AGT]GC[ACT]^C
1662 GTGCCC
BsaBI 139 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1348 GATATTTATC
BsiYI 64 CCAGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2091 CCTAAGCATGG
BslI 64 CCAGAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2091 CCTAAGCATGG
BsmI 771 GAATGC GAATGC(1/-1)
18 GCATTC
Bsp1286I 539 GTGCAC G[AGT]GC[ACT]^C
1662 GTGCCC
BssSI 2180 CACGAG CACGAG(-5/-1)
1958 CTCGTG
BstYI 1245 AGATCC [GA]^GATC[CT]
1269 AGATCT
FbaI 1274 TGATCA T^GATCA
1449 TGATCA
MamI 139 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1348 GATATTTATC
MflI 1245 AGATCC [GA]^GATC[CT]
1269 AGATCT
MwoI 994 GCGGTGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1396 GCAATATTAGC
NspI 498 ACATGC [GA]CATG^[CT]
623 GCATGT
PleI 1371 GACTC GAGTC(4/5)
1637 GACTC
TspRI 1029 CAGTG CA[GC]TG[ACGT][ACGT]^
1978 CAGTG
XhoII 1245 AGATCC [GA]^GATC[CT]
1269 AGATCT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BciVI BfaI BsaJI BseRI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I