Restriction map for
pFN21AE3390
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# NO CUT ENZYMES ( 92 enzymes) #
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Acc65I AccI AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
BanI BbeI BcnI BglI BseAI
BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FauI FseI
FspI HgaI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PflFI PinAI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 2480 GACGTC GACGT^C
AccII 3334 CGCG CG^CG
AcyI 2480 GACGTC G[GA]^CG[CT]C
AflII 3022 CTTAAG C^TTAAG
AocI 1774 CCTGAGG CC^T[ACGT]AGG
AvaI 2915 CTCGAG C^[CT]CG[GA]G
AvrII 3457 CCTAGG C^CTAGG
BalI 3155 TGGCCA TGG^CCA
BamHI 314 GGATCC G^GATCC
BbrPI 67 CACGTG CAC^GTG
BcgI 2685 GCACACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1701 GGATAC GTATCC(6/5)
BfrI 3022 CTTAAG C^TTAAG
BlnI 3457 CCTAGG C^CTAGG
BlpI 3271 GCTCAGC GC^T[ACGT]AGC
BpmI 1517 CTGGAG CTGGAG(16/14)
Bpu1102I 3271 GCTCAGC GC^T[ACGT]AGC
BsaAI 67 CACGTG [CT]AC^GT[GA]
BsaBI 2333 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2480 GACGTC G[GA]^CG[CT]C
BsaWI 3604 ACCGGA [AT]^CCGG[AT]
BseRI 970 GAGGAG GAGGAG(10/8)
BsoBI 2915 CTCGAG C^[CT]CG[GA]G
Bsp1407I 143 TGTACA T^GTACA
BspDI 2334 ATCGAT AT^CGAT
BspLU11I 1651 ACATGT A^CATGT
BsrDI 1222 GCAATG GCAATG(2/0)
BsrGI 143 TGTACA T^GTACA
BstUI 3334 CGCG CG^CG
BstXI 772 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1774 CCTGAGG CC^T[ACGT]AGG
CelII 3271 GCTCAGC GC^T[ACGT]AGC
ClaI 2334 ATCGAT AT^CGAT
DraI 3517 TTTAAA TTT^AAA
DrdI 3525 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1763 CCATGG C^C[GA][CT]GG
EaeI 3155 TGGCCA [CT]^GGCC[GA]
Eco81I 1774 CCTGAGG CC^T[ACGT]AGG
EcoT22I 422 ATGCAT ATGCA^T
Hin1I 2480 GACGTC G[GA]^CG[CT]C
MamI 2333 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3155 TGGCCA TGG^CCA
MscI 3155 TGGCCA TGG^CCA
MslI 773 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 3334 CGCG CG^CG
NcoI 1763 CCATGG C^CATGG
NsiI 422 ATGCAT ATGCA^T
NspI 1651 ACATGT [GA]CATG^[CT]
PacI 1750 TTAATTAA TTAAT^TAA
PaeR7I 2915 CTCGAG C^TCGAG
PflMI 3366 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 67 CACGTG CAC^GTG
PmlI 67 CACGTG CAC^GTG
SapI 2518 GAAGAGC GCTCTTC(1/4)
SspBI 143 TGTACA T^GTACA
Tsp45I 2023 GTCAC ^GT[GC]AC
Van91I 3366 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3143 CCAAGACTCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2915 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 66 ACACGT A^C[GA][CT]GT
1651 ACATGT
Asp700I 557 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2197 GAAACTATTC
BbvCI 2113 CCTCAGC CCTCAGC(-5/-2)
1696 GCTGAGG
BclI 258 TGATCA T^GATCA
1622 TGATCA
BsgI 3233 CTGCAC GTGCAG(16/14)
332 GTGCAG
BsmI 3538 GAATGC GAATGC(1/-1)
3597 GCATTC
BspHI 705 TCATGA T^CATGA
2906 TCATGA
BspMI 999 ACCTGC ACCTGC(4/8)
334 GCAGGT
BssSI 540 CACGAG CACGAG(-5/-1)
3031 CACGAG
BstAPI 343 GCAGTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3342 GCATCAGCTGC
DraII 77 GGGACCT [GA]G^G[ACGT]CC[CT]
1770 GGGTCCT
EcoO109I 77 GGGACCT [GA]G^G[ACGT]CC[CT]
1770 GGGTCCT
EcoRV 502 GATATC GAT^ATC
2337 GATATC
EcoT14I 1763 CCATGG C^C[AT][AT]GG
3457 CCTAGG
FbaI 258 TGATCA T^GATCA
1622 TGATCA
HaeII 2582 GGCGCT [GA]GCGC^[CT]
3026 AGCGCC
HphI 1915 GGTGA GGTGA(8/7)
261 TCACC
PpuMI 77 GGGACCT [GA]G^G[AT]CC[CT]
1770 GGGTCCT
PstI 859 CTGCAG CTGCA^G
3349 CTGCAG
RcaI 705 TCATGA T^CATGA
2906 TCATGA
SacI 2912 GAGCTC GAGCT^C
2935 GAGCTC
StyI 1763 CCATGG C^C[AT][AT]GG
3457 CCTAGG
XbaI 282 TCTAGA T^CTAGA
2640 TCTAGA
XmnI 557 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2197 GAAACTATTC
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaII BanII BbsI BbvI BfaI
BglII BmyI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII