Restriction map for
pFN21AB9761
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI ApaI AscI Asp718I
AspI AviII AvrII BanI BbeI
BbrPI BbvCI BciVI BfrI BlnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrDI BssSI Bst1107I BstBI
BstZ17I CelII ClaI DraI DrdI
EagI EclXI Eco52I EcoRI EcoT22I
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PvuI SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AclI 223 AACGTT AA^CGTT
AcsI 55 GAATTT [GA]^AATT[CT]
AflIII 590 ACATGT A^C[GA][CT]GT
AhdI 1612 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 470 AGCGCT AGC^GCT
ApoI 55 GAATTT [GA]^AATT[CT]
AseI 1783 ATTAAT AT^TAAT
AsnI 1783 ATTAAT AT^TAAT
AspEI 1612 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1359 CTCGAG C^[CT]CG[GA]G
BalI 503 TGGCCA TGG^CCA
BamHI 1562 GGATCC G^GATCC
BbsI 786 GAAGAC GAAGAC(2/6)
BclI 1200 TGATCA T^GATCA
BglI 1211 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1037 AGATCT A^GATCT
BpuAI 786 GAAGAC GAAGAC(2/6)
BsaBI 1190 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2066 GAGACC GGTCTC(1/5)
BsaWI 358 ACCGGA [AT]^CCGG[AT]
BsmI 2034 GCATTC GAATGC(1/-1)
BsoBI 1359 CTCGAG C^[CT]CG[GA]G
Bsp1407I 305 TGTACA T^GTACA
BspHI 1203 TCATGA T^CATGA
BspLU11I 590 ACATGT A^CATGT
BsrGI 305 TGTACA T^GTACA
BssHII 836 GCGCGC G^CGCGC
BstAPI 840 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 209 CCAGCCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1319 CGGACCG CG^G[AT]CCG
DraII 773 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1612 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 470 AGCGCT AGC^GCT
EcoNI 1491 CCTGACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 773 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1643 GATATC GAT^ATC
FauI 1264 CCCGC CCCGC(4/6)
FbaI 1200 TGATCA T^GATCA
HincII 982 GTCAAC GT[CT]^[GA]AC
HindII 982 GTCAAC GT[CT]^[GA]AC
MamI 1190 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 503 TGGCCA TGG^CCA
MscI 503 TGGCCA TGG^CCA
NaeI 517 GCCGGC GCC^GGC
NgoMIV 517 GCCGGC G^CCGGC
PaeR7I 1359 CTCGAG C^TCGAG
PpuMI 773 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1945 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1783 ATTAAT AT^TAAT
Psp1406I 223 AACGTT AA^CGTT
PvuII 106 CAGCTG CAG^CTG
RcaI 1203 TCATGA T^CATGA
RsrII 1319 CGGACCG CG^G[AT]CCG
ScaI 878 AGTACT AGT^ACT
SspBI 305 TGTACA T^GTACA
XhoI 1359 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 805 GTGCAC G^TGCAC
940 GTGCAC
AocI 483 CCTGAGG CC^T[ACGT]AGG
769 CCTCAGG
ApaLI 805 GTGCAC G^TGCAC
940 GTGCAC
Asp700I 376 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2183 GAAACTATTC
BcgI 932 CGACCATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
942 GCACAACAGTCG
BfaI 32 CTAG C^TAG
2017 CTAG
BseRI 954 CTCCTC GAGGAG(10/8)
2048 CTCCTC
BsgI 451 GTGCAG GTGCAG(16/14)
919 GTGCAG
BspMI 827 ACCTGC ACCTGC(4/8)
1424 GCAGGT
BsrFI 517 GCCGGC [GA]^CCGG[CT]
2105 ACCGGC
BstEII 867 GGTGACC G^GT[ACGT]ACC
1636 GGTGACC
BstPI 867 GGTGACC G^GT[ACGT]ACC
1636 GGTGACC
Bsu36I 483 CCTGAGG CC^T[ACGT]AGG
769 CCTCAGG
Cfr10I 517 GCCGGC [GA]^CCGG[CT]
2105 ACCGGC
EarI 1372 GAAGAG CTCTTC(1/4)
1732 GAAGAG
Eco81I 483 CCTGAGG CC^T[ACGT]AGG
769 CCTCAGG
EcoO65I 867 GGTGACC G^GT[ACGT]ACC
1636 GGTGACC
HaeII 470 AGCGCT [GA]GCGC^[CT]
658 AGCGCC
Ksp632I 1372 GAAGAG CTCTTC(1/4)
1732 GAAGAG
MaeI 32 CTAG C^TAG
2017 CTAG
MaeII 224 ACGT A^CGT
620 ACGT
MspA1I 106 CAGCTG C[AC]G^C[GT]G
555 CAGCGG
NcoI 1566 CCATGG C^CATGG
1857 CCATGG
PstI 552 CTGCAG CTGCA^G
1018 CTGCAG
SmlI 198 CTCAAG C^T[CT][GA]AG
1359 CTCGAG
SphI 1535 GCATGC GCATG^C
2124 GCATGC
TfiI 1195 GAATC G^A[AT]TC
1630 GAATC
XcmI 255 CCAGACCCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1557 CCACAGGATCCATGG
XmnI 376 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2183 GAAACTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraIII DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII