Restriction map for
pFN21AE2667
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbrPI BciVI BclI BfrI
BglII BlnI BsiEI BsiWI Bsp1407I
BspHI BspLU11I BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI DraI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FspI HindIII
HpaI KpnI MfeI MluI MseI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI SapI
SbfI SfiI SfuI SgrAI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 73 GTATAC GT^[AC][GT]AC
AccIII 803 TCCGGA T^CCGGA
AcsI 1288 GAATTC [GA]^AATT[CT]
ApoI 1288 GAATTC [GA]^AATT[CT]
Asp700I 3267 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 618 GAGCAC G[AT]GC[AT]^C
BcgI 3015 CGAACGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2360 CTAG C^TAG
BsaBI 2232 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 803 TCCGGA T^CCGGA
BsiHKAI 618 GAGCAC G[AT]GC[AT]^C
BsmBI 3147 CGTCTC CGTCTC(1/5)
BspEI 803 TCCGGA T^CCGGA
Bst1107I 73 GTATAC GTA^TAC
BstZ17I 73 GTATAC GTA^TAC
CpoI 2180 CGGTCCG CG^G[AT]CCG
DraIII 335 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1102 GACAGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1288 GAATTC G^AATTC
FseI 918 GGCCGGCC GGCCGG^CC
MaeI 2360 CTAG C^TAG
MamI 2232 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 803 TCCGGA T^CCGGA
NcoI 3206 CCATGG C^CATGG
NdeI 1796 CATATG CA^TATG
NspI 2455 ACATGC [GA]CATG^[CT]
RsrII 2180 CGGTCCG CG^G[AT]CCG
ScaI 1271 AGTACT AGT^ACT
SexAI 91 ACCTGGT A^CC[AT]GGT
SnaBI 70 TACGTA TAC^GTA
Tsp509I 1289 AATT ^AATT
XmnI 3267 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 43 enzymes) #
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AhdI 1807 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2154 GACCTCCTGTC
AocI 1655 CCTCAGG CC^T[ACGT]AGG
2795 CCTCAGG
AspEI 1807 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2154 GACCTCCTGTC
AspI 90 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
343 GACGAAGTC
BalI 186 TGGCCA TGG^CCA
1142 TGGCCA
BbeI 858 GGCGCC GGCGC^C
2252 GGCGCC
BbsI 349 GTCTTC GAAGAC(2/6)
1737 GTCTTC
BbvCI 1156 CCTCAGC CCTCAGC(-5/-2)
1973 CCTCAGC
BlpI 552 GCTGAGC GC^T[ACGT]AGC
1626 GCTGAGC
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
1626 GCTGAGC
BpuAI 349 GTCTTC GAAGAC(2/6)
1737 GTCTTC
BsaI 1765 GAGACC GGTCTC(1/5)
2262 GAGACC
BsmI 1941 GAATGC GAATGC(1/-1)
1600 GCATTC
BspDI 289 ATCGAT AT^CGAT
1420 ATCGAT
BstYI 233 AGATCC [GA]^GATC[CT]
3251 AGATCC
Bsu36I 1655 CCTCAGG CC^T[ACGT]AGG
2795 CCTCAGG
CelII 552 GCTGAGC GC^T[ACGT]AGC
1626 GCTGAGC
ClaI 289 ATCGAT AT^CGAT
1420 ATCGAT
Eam1105I 1807 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2154 GACCTCCTGTC
Eco81I 1655 CCTCAGG CC^T[ACGT]AGG
2795 CCTCAGG
EcoNI 873 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1755 CCTGGAAGAGG
HincII 382 GTCAAC GT[CT]^[GA]AC
2731 GTCAAC
HindII 382 GTCAAC GT[CT]^[GA]AC
2731 GTCAAC
KasI 858 GGCGCC G^GCGCC
2252 GGCGCC
MflI 233 AGATCC [GA]^GATC[CT]
3251 AGATCC
MluNI 186 TGGCCA TGG^CCA
1142 TGGCCA
MscI 186 TGGCCA TGG^CCA
1142 TGGCCA
MslI 27 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3072 CATACCCGTG
NaeI 919 GCCGGC GCC^GGC
1695 GCCGGC
NarI 858 GGCGCC GG^CGCC
2252 GGCGCC
NgoMIV 919 GCCGGC G^CCGGC
1695 GCCGGC
PflFI 90 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
343 GACGAAGTC
PflMI 2589 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3249 CCAGATCCTGG
PpuMI 194 AGGACCC [GA]G^G[AT]CC[CT]
641 GGGTCCC
PstI 148 CTGCAG CTGCA^G
1708 CTGCAG
SfcI 148 CTGCAG C^T[GA][CT]AG
1708 CTGCAG
SfoI 858 GGCGCC GGC^GCC
2252 GGCGCC
SmaI 2165 CCCGGG CCC^GGG
2776 CCCGGG
Tth111I 90 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
343 GACGAAGTC
Van91I 2589 CCACTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3249 CCAGATCCTGG
XhoII 233 AGATCC [GA]^GATC[CT]
3251 AGATCC
XmaCI 2165 CCCGGG C^CCGGG
2776 CCCGGG
XmaI 2165 CCCGGG C^CCGGG
2776 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XcmI