##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssKI       BssSI       Bst1107I    BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraI        DraIII      DrdI        DsaI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MslI        MspA1I      
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AciI          235  GCGG                 CCGC(-3/-1)
AfaI          156  GTAC                 GT^AC
AspHI         130  GAGCTC               G[AT]GC[AT]^C
BalI           59  TGGCCA               TGG^CCA
BanI          280  GGTGCC               G^G[CT][GA]CC
BanII         130  GAGCTC               G[GA]GC[CT]^C
BseRI         127  GAGGAG               GAGGAG(10/8)
BsiHKAI       130  GAGCTC               G[AT]GC[AT]^C
BsrDI         184  GCAATG               GCAATG(2/0)
BsrI          111  ACTGG                ACTGG(1/-1)
BstAPI        219  GCAGCCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I         56  GGATG                GGATG(2/0)
Cac8I         215  GCCTGC               GC[ACGT]^[ACGT]GC
EaeI           59  TGGCCA               [CT]^GGCC[GA]
FokI           56  GGATG                GGATG(9/13)
HapII          54  CCGG                 C^CGG
HincII        163  GTCAAC               GT[CT]^[GA]AC
HindII        163  GTCAAC               GT[CT]^[GA]AC
HpaII          54  CCGG                 C^CGG
HphI           35  TCACC                GGTGA(8/7)
MboII         169  GAAGA                GAAGA(8/7)
MluNI          59  TGGCCA               TGG^CCA
MscI           59  TGGCCA               TGG^CCA
MspI           54  CCGG                 C^CGG
NlaIII         90  CATG                 CATG^
RsaI          156  GTAC                 GT^AC
SacI          130  GAGCTC               GAGCT^C
SmlI          196  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaII         119  GGACC                G^G[AT]CC
              143  GGACC               
BmyI           14  GTGCCC               G[AGT]GC[ACT]^C
              130  GAGCTC              
BsaJI         146  CCTTGG               C^C[ACGT][ACGT]GG
              176  CCAAGG              
BsiYI          18  CCTCCTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               21  CCTGCTGGTGG         
BslI           18  CCTCCTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               21  CCTGCTGGTGG         
BsmFI         118  GGGAC                GGGAC(10/14)
              142  GGGAC               
Bsp1286I       14  GTGCCC               G[AGT]GC[ACT]^C
              130  GAGCTC              
DraII         118  GGGACCC              [GA]G^G[ACGT]CC[CT]
              142  GGGACCT             
EcoO109I      118  GGGACCC              [GA]G^G[ACGT]CC[CT]
              142  GGGACCT             
EcoT14I       146  CCTTGG               C^C[AT][AT]GG
              176  CCAAGG              
PpuMI         118  GGGACCC              [GA]G^G[AT]CC[CT]
              142  GGGACCT             
PstI          204  CTGCAG               CTGCA^G
              217  CTGCAG              
SfaNI         172  GATGC                GCATC(5/9)
               47  GCATC               
SfcI          204  CTGCAG               C^T[GA][CT]AG
              217  CTGCAG              
StyI          146  CCTTGG               C^C[AT][AT]GG
              176  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AluI        BbvI        Cfr13I      Fnu4HI      HaeIII      
ItaI        MnlI        MwoI        NlaIV       Sau96I      
TseI