Restriction map for
pFN21AE6135
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DpnII DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI Ksp632I MaeI
MamI MboI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MslI MspI MunI NaeI NarI
NciI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 211 AAATTT [GA]^AATT[CT]
ApaI 203 GGGCCC GGGCC^C
ApoI 211 AAATTT [GA]^AATT[CT]
AscI 164 GGCGCGCC GG^CGCGCC
BanII 203 GGGCCC G[GA]GC[CT]^C
BlpI 267 GCTGAGC GC^T[ACGT]AGC
BmyI 203 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 267 GCTGAGC GC^T[ACGT]AGC
BsaI 186 GGTCTC GGTCTC(1/5)
BseRI 262 GAGGAG GAGGAG(10/8)
BsiWI 155 CGTACG C^GTACG
BsmAI 187 GTCTC GTCTC(1/5)
Bsp1286I 203 GGGCCC G[AGT]GC[ACT]^C
BsrBI 160 GAGCGG CCGCTC(-3/-3)
BsrI 279 ACTGG ACTGG(1/-1)
BssHII 165 GCGCGC G^CGCGC
BssKI 71 CCAGG ^CC[ACGT]GG
BstF5I 240 CATCC GGATG(2/0)
BstNI 71 CCAGG CC^[AT]GG
CelII 267 GCTGAGC GC^T[ACGT]AGC
DdeI 268 CTGAG C^T[ACGT]AG
DraII 203 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 43 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 30 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 203 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 71 CCAGG ^CC[AT]GG
FokI 240 CATCC GGATG(9/13)
HinfI 129 GATTC G^A[ACGT]TC
KspI 87 CCGCGG CCGC^GG
MspA1I 87 CCGCGG C[AC]G^C[GT]G
MvaI 71 CCAGG CC^[AT]GG
NcoI 170 CCATGG C^CATGG
NlaIV 203 GGGCCC GG[ACGT]^[ACGT]CC
SacII 87 CCGCGG CCGC^GG
ScrFI 71 CCAGG CC^[ACGT]GG
TfiI 129 GATTC G^A[AT]TC
Tsp45I 49 GTGAC ^GT[GC]AC
Tsp509I 212 AATT ^AATT
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 83 GACGCC G[GA]^CG[CT]C
92 GGCGTC
AfaI 156 GTAC GT^AC
277 GTAC
AluI 266 AGCT AG^CT
297 AGCT
BbvI 298 GCTGC GCAGC(8/12)
301 GCTGC
BsaHI 83 GACGCC G[GA]^CG[CT]C
92 GGCGTC
BsiYI 30 CCTCAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
284 CCGCCATGAGG
BslI 30 CCTCAACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
284 CCGCCATGAGG
BsmFI 122 GGGAC GGGAC(10/14)
232 GGGAC
EaeI 20 TGGCCG [CT]^GGCC[GA]
281 TGGCCG
EcoT14I 170 CCATGG C^C[AT][AT]GG
191 CCAAGG
Hin1I 83 GACGCC G[GA]^CG[CT]C
92 GGCGTC
MaeII 59 ACGT A^CGT
275 ACGT
MaeIII 49 GTGAC ^GT[ACGT]AC
177 GTTAC
MwoI 80 GCCGACGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
258 GCGGGAGGAGC
RsaI 156 GTAC GT^AC
277 GTAC
StyI 170 CCATGG C^C[AT][AT]GG
191 CCAAGG
TseI 298 GCTGC G^C[AT]GC
301 GCTGC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AccII AciI BsaJI BstUI Cac8I
CfoI Cfr13I DsaI FauI Fnu4HI
HaeIII HgaI HhaI HinP1I ItaI
MnlI MvnI NlaIII Sau96I