Restriction map for
pFN21AE1117
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AfaI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbsI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 709 GTAGAC GT^[AC][GT]AC
AclI 760 AACGTT AA^CGTT
AcsI 48 AAATTT [GA]^AATT[CT]
AflIII 488 ACGTGT A^C[GA][CT]GT
Alw44I 63 GTGCAC G^TGCAC
AocI 426 CCTCAGG CC^T[ACGT]AGG
ApaLI 63 GTGCAC G^TGCAC
ApoI 48 AAATTT [GA]^AATT[CT]
Asp700I 592 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 63 GTGCAC G[AT]GC[AT]^C
AvaI 906 CCCGGG C^[CT]CG[GA]G
AvaII 573 GGACC G^G[AT]CC
BamHI 614 GGATCC G^GATCC
BanI 687 GGTGCC G^G[CT][GA]CC
BbvCI 820 GCTGAGG CCTCAGC(-5/-2)
BcgI 439 CGAAGTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 375 GGATAC GTATCC(6/5)
BglI 273 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 249 GGTCTC GGTCTC(1/5)
BsiHKAI 63 GTGCAC G[AT]GC[AT]^C
BsoBI 906 CCCGGG C^[CT]CG[GA]G
Bsu36I 426 CCTCAGG CC^T[ACGT]AGG
CfoI 170 GCGC GCG^C
DraII 572 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 574 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 528 TGGCCG [CT]^GGCC[GA]
Eco81I 426 CCTCAGG CC^T[ACGT]AGG
EcoO109I 572 GGGACCT [GA]G^G[ACGT]CC[CT]
HapII 907 CCGG C^CGG
HgaI 434 GCGTC GACGC(5/10)
HhaI 170 GCGC GCG^C
HinP1I 170 GCGC G^CGC
HpaII 907 CCGG C^CGG
MspI 907 CCGG C^CGG
NspI 752 GCATGC [GA]CATG^[CT]
PflMI 859 CCATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 581 GAGTC GAGTC(4/5)
PpuMI 572 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 760 AACGTT AA^CGTT
PvuII 114 CAGCTG CAG^CTG
SmaI 906 CCCGGG CCC^GGG
SmlI 882 CTTGAG C^T[CT][GA]AG
SphI 752 GCATGC GCATG^C
Van91I 859 CCATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 138 TCTAGA T^CTAGA
XcmI 494 CCATTTGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 906 CCCGGG C^CCGGG
XmaI 906 CCCGGG C^CCGGG
XmnI 592 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BcnI 906 CCCGG CC^[GC]GG
907 CCGGG
BfaI 139 CTAG C^TAG
286 CTAG
BseRI 848 CTCCTC GAGGAG(10/8)
823 GAGGAG
BsgI 92 CTGCAC GTGCAG(16/14)
832 CTGCAC
BsmAI 250 GTCTC GTCTC(1/5)
814 GTCTC
BstYI 222 GGATCT [GA]^GATC[CT]
614 GGATCC
Cfr13I 272 GGCCC G^G[ACGT]CC
573 GGACC
DpnII 223 GATC ^GATC
615 GATC
DsaI 213 CCATGG C^C[GA][CT]GG
245 CCATGG
EarI 97 CTCTTC CTCTTC(1/4)
703 CTCTTC
Ksp632I 97 CTCTTC CTCTTC(1/4)
703 CTCTTC
MaeI 139 CTAG C^TAG
286 CTAG
MboI 223 GATC ^GATC
615 GATC
MflI 222 GGATCT [GA]^GATC[CT]
614 GGATCC
MspA1I 114 CAGCTG C[AC]G^C[GT]G
691 CCGCTG
NciI 906 CCCGG CC^[GC]GG
907 CCGGG
NcoI 213 CCATGG C^CATGG
245 CCATGG
NdeII 223 GATC ^GATC
615 GATC
Sau3AI 223 GATC ^GATC
615 GATC
Sau96I 272 GGCCC G^G[ACGT]CC
573 GGACC
Tsp45I 12 GTCAC ^GT[GC]AC
19 GTCAC
Tsp509I 49 AATT ^AATT
193 AATT
XhoII 222 GGATCT [GA]^GATC[CT]
614 GGATCC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I DdeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeII MaeIII MboII MnlI
MvaI MwoI NlaIII NlaIV PstI
ScrFI SfaNI SfcI StyI TfiI
TseI TspRI