Restriction map for
pFN21AE1496
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HapII
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tru9I Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 48 CGCG CG^CG
AcsI 304 GAATTC [GA]^AATT[CT]
AcyI 180 GGCGTC G[GA]^CG[CT]C
AfaI 203 GTAC GT^AC
AluI 171 AGCT AG^CT
AlwI 150 GGATC GGATC(4/5)
Aor51HI 59 AGCGCT AGC^GCT
ApoI 304 GAATTC [GA]^AATT[CT]
AvaI 27 CCCGAG C^[CT]CG[GA]G
BanI 34 GGCACC G^G[CT][GA]CC
BbvI 7 GCAGC GCAGC(8/12)
BciVI 21 GGATAC GTATCC(6/5)
BglI 91 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 319 GGGCAC G[AGT]GC[ACT]^C
BsaHI 180 GGCGTC G[GA]^CG[CT]C
BsaJI 101 CCTGGG C^C[ACGT][ACGT]GG
BsmI 81 GAATGC GAATGC(1/-1)
BsoBI 27 CCCGAG C^[CT]CG[GA]G
Bsp1286I 319 GGGCAC G[AGT]GC[ACT]^C
BsrBI 30 GAGCGG CCGCTC(-3/-3)
BstF5I 15 GGATG GGATG(2/0)
BstUI 48 CGCG CG^CG
BstXI 309 CCAAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 150 GGATCT [GA]^GATC[CT]
Cfr13I 98 GGGCC G^G[ACGT]CC
DpnII 151 GATC ^GATC
DrdI 272 GACAGGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 196 GAAGAG CTCTTC(1/4)
Eco47III 59 AGCGCT AGC^GCT
EcoRI 304 GAATTC G^AATTC
FauI 49 GCGGG CCCGC(4/6)
FokI 15 GGATG GGATG(9/13)
HaeII 59 AGCGCT [GA]GCGC^[CT]
HaeIII 99 GGCC GG^CC
HgaI 181 GCGTC GACGC(5/10)
Hin1I 180 GGCGTC G[GA]^CG[CT]C
HinfI 218 GAGTC G^A[ACGT]TC
Ksp632I 196 GAAGAG CTCTTC(1/4)
MaeIII 224 GTAAC ^GT[ACGT]AC
MboI 151 GATC ^GATC
MboII 196 GAAGA GAAGA(8/7)
MflI 150 GGATCT [GA]^GATC[CT]
MnlI 93 CCTC CCTC(7/6)
MspA1I 42 CAGCGG C[AC]G^C[GT]G
MvnI 48 CGCG CG^CG
NdeII 151 GATC ^GATC
PflMI 141 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 218 GAGTC GAGTC(4/5)
PstI 132 CTGCAG CTGCA^G
RsaI 203 GTAC GT^AC
Sau3AI 151 GATC ^GATC
Sau96I 98 GGGCC G^G[ACGT]CC
SfaNI 248 GCATC GCATC(5/9)
SfcI 132 CTGCAG C^T[GA][CT]AG
TseI 7 GCAGC G^C[AT]GC
Tsp509I 305 AATT ^AATT
TspRI 244 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 141 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 150 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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BsiYI 128 CCATCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
141 CCACATTATGG
BslI 128 CCATCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
141 CCACATTATGG
BssKI 101 CCTGG ^CC[ACGT]GG
177 CCAGG
BstNI 101 CCTGG CC^[AT]GG
177 CCAGG
Cac8I 49 GCGGGC GC[ACGT]^[ACGT]GC
257 GCTGGC
CfoI 47 GCGC GCG^C
60 GCGC
EcoRII 101 CCTGG ^CC[AT]GG
177 CCAGG
HhaI 47 GCGC GCG^C
60 GCGC
HinP1I 47 GCGC G^CGC
60 GCGC
MvaI 101 CCTGG CC^[AT]GG
177 CCAGG
NlaIII 212 CATG CATG^
229 CATG
NlaIV 34 GGCACC GG[ACGT]^[ACGT]CC
137 GGAACC
ScrFI 101 CCTGG CC^[ACGT]GG
177 CCAGG
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
AciI Fnu4HI ItaI MwoI