Restriction map for
pFN21AE6147
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssSI BstAPI
BstBI BstEII BstPI BstXI BstYI
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MseI MslI MspA1I MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
TaqI Tru9I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 394 GTATAC GT^[AC][GT]AC
AccII 63 CGCG CG^CG
AcsI 85 AAATTT [GA]^AATT[CT]
AluI 330 AGCT AG^CT
ApoI 85 AAATTT [GA]^AATT[CT]
BalI 141 TGGCCA TGG^CCA
BclI 264 TGATCA T^GATCA
BpmI 352 CTCCAG CTGGAG(16/14)
BsaJI 144 CCATGG C^C[ACGT][ACGT]GG
BseRI 241 GAGGAG GAGGAG(10/8)
Bsp1407I 77 TGTACA T^GTACA
BsrGI 77 TGTACA T^GTACA
BssKI 30 CCAGG ^CC[ACGT]GG
Bst1107I 394 GTATAC GTA^TAC
BstF5I 285 GGATG GGATG(2/0)
BstNI 30 CCAGG CC^[AT]GG
BstUI 63 CGCG CG^CG
BstZ17I 394 GTATAC GTA^TAC
Cac8I 64 GCGAGC GC[ACGT]^[ACGT]GC
CfoI 62 GCGC GCG^C
DdeI 91 CTAAG C^T[ACGT]AG
DsaI 144 CCATGG C^C[GA][CT]GG
EcoRII 30 CCAGG ^CC[AT]GG
EcoT14I 144 CCATGG C^C[AT][AT]GG
FauI 44 CCCGC CCCGC(4/6)
FbaI 264 TGATCA T^GATCA
FokI 285 GGATG GGATG(9/13)
HhaI 62 GCGC GCG^C
HinP1I 62 GCGC G^CGC
MaeIII 169 GTGAC ^GT[ACGT]AC
MluNI 141 TGGCCA TGG^CCA
MscI 141 TGGCCA TGG^CCA
MvaI 30 CCAGG CC^[AT]GG
MvnI 63 CGCG CG^CG
MwoI 20 GCCGTCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 144 CCATGG C^CATGG
ScrFI 30 CCAGG CC^[ACGT]GG
SfaNI 50 GCATC GCATC(5/9)
SfcI 368 CTGTAG C^T[GA][CT]AG
SspBI 77 TGTACA T^GTACA
SspI 412 AATATT AAT^ATT
StyI 144 CCATGG C^C[AT][AT]GG
Tsp45I 169 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 45 CCGC CCGC(-3/-1)
48 CCGC
AfaI 78 GTAC GT^AC
175 GTAC
AspHI 3 GAGCAC G[AT]GC[AT]^C
312 GAGCAC
BbvI 255 GCAGC GCAGC(8/12)
26 GCTGC
BsiHKAI 3 GAGCAC G[AT]GC[AT]^C
312 GAGCAC
BsiYI 192 CCCTGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
193 CCTGCTACGGG
BslI 192 CCCTGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
193 CCTGCTACGGG
BsmFI 344 GGGAC GGGAC(10/14)
42 GTCCC
DpnII 223 GATC ^GATC
265 GATC
EaeI 18 TGGCCG [CT]^GGCC[GA]
141 TGGCCA
HaeIII 19 GGCC GG^CC
142 GGCC
HinfI 213 GAATC G^A[ACGT]TC
273 GAATC
HphI 168 GGTGA GGTGA(8/7)
309 GGTGA
MboI 223 GATC ^GATC
265 GATC
MboII 100 GAAGA GAAGA(8/7)
363 TCTTC
NdeII 223 GATC ^GATC
265 GATC
NlaIII 145 CATG CATG^
178 CATG
RsaI 78 GTAC GT^AC
175 GTAC
Sau3AI 223 GATC ^GATC
265 GATC
TfiI 213 GAATC G^A[AT]TC
273 GAATC
TseI 26 GCTGC G^C[AT]GC
255 GCAGC
Tsp509I 86 AATT ^AATT
154 AATT
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
BmyI Bsp1286I Fnu4HI ItaI MnlI