##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AlwNI         316  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BamHI          62  GGATCC               G^GATCC
BbsI          222  GAAGAC               GAAGAC(2/6)
BcnI          414  CCGGG                CC^[GC]GG
BfaI          148  CTAG                 C^TAG
BpuAI         222  GAAGAC               GAAGAC(2/6)
BsaBI          45  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          234  GTGCAG               GTGCAG(16/14)
BstYI          62  GGATCC               [GA]^GATC[CT]
CfoI          360  GCGC                 GCG^C
DpnII          63  GATC                 ^GATC
DsaI          176  CCACGG               C^C[GA][CT]GG
FauI           59  GCGGG                CCCGC(4/6)
HapII         414  CCGG                 C^CGG
HhaI          360  GCGC                 GCG^C
HinP1I        360  GCGC                 G^CGC
HinfI          45  GATTC                G^A[ACGT]TC
HpaII         414  CCGG                 C^CGG
HphI           30  GGTGA                GGTGA(8/7)
MaeI          148  CTAG                 C^TAG
MamI           45  GATTCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI           63  GATC                 ^GATC
MboII         222  GAAGA                GAAGA(8/7)
MflI           62  GGATCC               [GA]^GATC[CT]
MslI           11  CACTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        319  CAGCTG               C[AC]G^C[GT]G
MspI          414  CCGG                 C^CGG
NciI          414  CCGGG                CC^[GC]GG
NdeII          63  GATC                 ^GATC
NlaIII        199  CATG                 CATG^
PflMI         176  CCACGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         319  CAGCTG               CAG^CTG
Sau3AI         63  GATC                 ^GATC
TfiI           45  GATTC                G^A[AT]TC
Van91I        176  CCACGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII          62  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AluI          304  AGCT                 AG^CT
              320  AGCT                
AlwI           63  GATCC                GGATC(4/5)
               62  GGATC               
BstF5I        111  CATCC                GGATG(2/0)
              282  CATCC               
Cfr13I        125  GGCCC                G^G[ACGT]CC
              404  GGCCC               
FokI          111  CATCC                GGATG(9/13)
              282  CATCC               
NlaIV          62  GGATCC               GG[ACGT]^[ACGT]CC
              404  GGCCCC              
Sau96I        125  GGCCC                G^G[ACGT]CC
              404  GGCCC               
SfaNI         172  GATGC                GCATC(5/9)
              239  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstNI       EcoRII      Fnu4HI      
HaeIII      ItaI        MaeIII      MnlI        MvaI        
MwoI        ScrFI       TseI        Tsp45I      TspRI