Restriction map for
pFN21AE3515
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BseAI BsiWI
BspDI BspEI BspHI BspLU11I BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoT22I FbaI FseI FspI
HpaI KpnI MfeI MluI MluNI
MroI MscI MunI NdeI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 1039 GTCTAC GT^[AC][GT]AC
AlwNI 1609 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 985 GGATCC G^GATCC
BbvCI 25 CCTCAGC CCTCAGC(-5/-2)
BcgI 1859 GCACTCAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2368 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2540 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1283 TGTACA T^GTACA
BsrDI 1146 CATTGC GCAATG(2/0)
BsrGI 1283 TGTACA T^GTACA
BstAPI 1210 GCACAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoRI 1330 GAATTC G^AATTC
EcoRV 1927 GATATC GAT^ATC
EcoT14I 824 CCATGG C^C[AT][AT]GG
HincII 2810 GTCAAC GT[CT]^[GA]AC
HindII 2810 GTCAAC GT[CT]^[GA]AC
HindIII 2129 AAGCTT A^AGCTT
KspI 212 CCGCGG CCGC^GG
MamI 2368 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 824 CCATGG C^CATGG
NheI 1219 GCTAGC G^CTAGC
PvuII 1205 CAGCTG CAG^CTG
SacII 212 CCGCGG CCGC^GG
ScaI 1879 AGTACT AGT^ACT
SgrAI 463 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 1283 TGTACA T^GTACA
SspI 2271 AATATT AAT^ATT
StyI 824 CCATGG C^C[AT][AT]GG
XcmI 2769 CCAGATCAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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ApaI 480 GGGCCC GGGCC^C
1075 GGGCCC
Asp700I 2483 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2509 GAAGAGTTTC
BbsI 829 GAAGAC GAAGAC(2/6)
819 GTCTTC
BglI 379 GCCTCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
497 GCCCAGTCGGC
BglII 2103 AGATCT A^GATCT
2229 AGATCT
BpuAI 829 GAAGAC GAAGAC(2/6)
819 GTCTTC
BsaAI 2726 TACGTG [CT]AC^GT[GA]
2899 CACGTA
BsaI 2297 GAGACC GGTCTC(1/5)
595 GGTCTC
BsmBI 2336 GAGACG CGTCTC(1/5)
2529 GAGACG
BsmI 1487 GCATTC GAATGC(1/-1)
2550 GCATTC
BspMI 938 ACCTGC ACCTGC(4/8)
2872 GCAGGT
BsrBI 232 CCGCTC CCGCTC(-3/-3)
450 GAGCGG
BsrFI 80 GCCGGC [GA]^CCGG[CT]
464 GCCGGC
BssHII 65 GCGCGC G^CGCGC
404 GCGCGC
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
464 GCCGGC
DraI 808 TTTAAA TTT^AAA
1377 TTTAAA
DrdI 1761 GACAACCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2801 GACTAAATGGTC
HgaI 40 GACGC GACGC(5/10)
2581 GACGC
HphI 3 GGTGA GGTGA(8/7)
2526 GGTGA
NaeI 80 GCCGGC GCC^GGC
464 GCCGGC
NgoMIV 80 GCCGGC G^CCGGC
464 GCCGGC
NotI 48 GCGGCCGC GC^GGCCGC
184 GCGGCCGC
SacI 393 GAGCTC GAGCT^C
726 GAGCTC
SapI 765 GCTCTTC GCTCTTC(1/4)
1716 GCTCTTC
SmlI 1008 CTTGAG C^T[CT][GA]AG
1225 CTCAAG
XmnI 2483 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2509 GAAGAGTTTC
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI