Restriction map for
pFN21AE0555
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BclI BglI
BlnI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmFI
BsmI BspDI BspEI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MluI MroI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NspV PacI PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspI StuI SwaI Tth111I Van91I
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 70 enzymes) #
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AccI 802 GTAGAC GT^[AC][GT]AC
AflII 414 CTTAAG C^TTAAG
AlwNI 488 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1054 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 127 CTCGAG C^[CT]CG[GA]G
AvaII 240 GGTCC G^G[AT]CC
BanII 455 GAGCCC G[GA]GC[CT]^C
BbrPI 73 CACGTG CAC^GTG
BbvI 92 GCTGC GCAGC(8/12)
BcnI 167 CCGGG CC^[GC]GG
BfrI 414 CTTAAG C^TTAAG
BglII 1065 AGATCT A^GATCT
BlpI 440 GCTTAGC GC^T[ACGT]AGC
BmyI 455 GAGCCC G[AGT]GC[ACT]^C
BpmI 97 CTCCAG CTGGAG(16/14)
Bpu1102I 440 GCTTAGC GC^T[ACGT]AGC
BsaAI 73 CACGTG [CT]AC^GT[GA]
BsaBI 1066 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 485 GTGCAG GTGCAG(16/14)
BsmBI 407 GAGACG CGTCTC(1/5)
BsoBI 127 CTCGAG C^[CT]CG[GA]G
Bsp1286I 455 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 1116 TGTACA T^GTACA
BsrGI 1116 TGTACA T^GTACA
BstAPI 151 GCATTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 440 GCTTAGC GC^T[ACGT]AGC
Cfr13I 240 GGTCC G^G[ACGT]CC
DraII 239 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 291 CTCTTC CTCTTC(1/4)
EcoO109I 239 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1088 CCAAGG C^C[AT][AT]GG
EcoT22I 962 ATGCAT ATGCA^T
FauI 445 GCGGG CCCGC(4/6)
HincII 141 GTTGAC GT[CT]^[GA]AC
HindII 141 GTTGAC GT[CT]^[GA]AC
Ksp632I 291 CTCTTC CTCTTC(1/4)
MaeII 74 ACGT A^CGT
MaeIII 987 GTCAC ^GT[ACGT]AC
MamI 1066 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 146 CAATTG C^AATTG
MseI 415 TTAA T^TAA
MspA1I 463 CAGCTG C[AC]G^C[GT]G
MunI 146 CAATTG C^AATTG
NciI 167 CCGGG CC^[GC]GG
NheI 24 GCTAGC G^CTAGC
NlaIV 327 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 962 ATGCAT ATGCA^T
PaeR7I 127 CTCGAG C^TCGAG
PleI 1093 GACTC GAGTC(4/5)
PmaCI 73 CACGTG CAC^GTG
PmlI 73 CACGTG CAC^GTG
PpuMI 239 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 235 CTGCAG CTGCA^G
PvuII 463 CAGCTG CAG^CTG
SapI 290 GCTCTTC GCTCTTC(1/4)
Sau96I 240 GGTCC G^G[ACGT]CC
SbfI 234 CCTGCAGG CCTGCA^GG
SfaNI 952 GATGC GCATC(5/9)
SphI 960 GCATGC GCATG^C
Sse8387I 234 CCTGCAGG CCTGCA^GG
SspBI 1116 TGTACA T^GTACA
StyI 1088 CCAAGG C^C[AT][AT]GG
TaqI 128 TCGA T^CGA
Tru9I 415 TTAA T^TAA
TseI 92 GCTGC G^C[AT]GC
Tsp45I 987 GTCAC ^GT[GC]AC
TthHB8I 128 TCGA T^CGA
XbaI 1062 TCTAGA T^CTAGA
XhoI 127 CTCGAG C^TCGAG
XmnI 1054 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 851 AAATTT [GA]^AATT[CT]
1007 GAATTT
Aor51HI 89 AGCGCT AGC^GCT
113 AGCGCT
ApoI 851 AAATTT [GA]^AATT[CT]
1007 GAATTT
BalI 357 TGGCCA TGG^CCA
934 TGGCCA
BbsI 253 GAAGAC GAAGAC(2/6)
785 GAAGAC
BpuAI 253 GAAGAC GAAGAC(2/6)
785 GAAGAC
BsiYI 459 CCTTCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
912 CCTTGTAGGGG
BslI 459 CCTTCAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
912 CCTTGTAGGGG
BsmAI 130 GAGAC GTCTC(1/5)
407 GAGAC
BspMI 137 GCAGGT ACCTGC(4/8)
237 GCAGGT
BsrBI 33 GAGCGG CCGCTC(-3/-3)
46 GAGCGG
BstYI 974 GGATCT [GA]^GATC[CT]
1065 AGATCT
CfoI 90 GCGC GCG^C
114 GCGC
Eco47III 89 AGCGCT AGC^GCT
113 AGCGCT
HaeII 89 AGCGCT [GA]GCGC^[CT]
113 AGCGCT
HapII 109 CCGG C^CGG
167 CCGG
HhaI 90 GCGC GCG^C
114 GCGC
HinP1I 90 GCGC G^CGC
114 GCGC
HindIII 434 AAGCTT A^AGCTT
841 AAGCTT
HpaII 109 CCGG C^CGG
167 CCGG
MflI 974 GGATCT [GA]^GATC[CT]
1065 AGATCT
MluNI 357 TGGCCA TGG^CCA
934 TGGCCA
MscI 357 TGGCCA TGG^CCA
934 TGGCCA
MspI 109 CCGG C^CGG
167 CCGG
NspI 512 ACATGC [GA]CATG^[CT]
960 GCATGC
SfcI 235 CTGCAG C^T[GA][CT]AG
800 CTGTAG
TfiI 184 GATTC G^A[AT]TC
649 GAATC
XhoII 974 GGATCT [GA]^GATC[CT]
1065 AGATCT
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI AlwI BfaI
BsaJI BsrDI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SmlI
Tsp509I TspRI