Restriction map for
pFN21AA0300
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# NO CUT ENZYMES ( 43 enzymes) #
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AclI AgeI Alw44I ApaLI AscI
AviII AvrII BlnI BsiWI BspDI
BspLU11I BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI EcoT22I FseI
FspI HindIII MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmeI Psp1406I RsrII
SalI SexAI SfiI SfuI SgrAI
SnaBI SpeI SwaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 3037 GACGTC GACGT^C
Acc65I 2533 GGTACC G^GTACC
AflII 1698 CTTAAG C^TTAAG
AflIII 4810 ACACGT A^C[GA][CT]GT
Asp718I 2533 GGTACC G^GTACC
AspI 8282 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1586 GGATCC G^GATCC
BbrPI 4811 CACGTG CAC^GTG
BciVI 3131 GTATCC GTATCC(6/5)
BfrI 1698 CTTAAG C^TTAAG
BsaAI 4811 CACGTG [CT]AC^GT[GA]
DraI 5404 TTTAAA TTT^AAA
EagI 2430 CGGCCG C^GGCCG
EclXI 2430 CGGCCG C^GGCCG
Eco52I 2430 CGGCCG C^GGCCG
EcoRV 6889 GATATC GAT^ATC
HpaI 1281 GTTAAC GTT^AAC
KpnI 2533 GGTACC GGTAC^C
KspI 2319 CCGCGG CCGC^GG
NcoI 5114 CCATGG C^CATGG
NheI 5104 GCTAGC G^CTAGC
PflFI 8282 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2203 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4811 CACGTG CAC^GTG
PmlI 4811 CACGTG CAC^GTG
PshAI 5940 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 6243 CGATCG CGAT^CG
SacII 2319 CCGCGG CCGC^GG
SbfI 66 CCTGCAGG CCTGCA^GG
ScaI 2600 AGTACT AGT^ACT
SphI 7466 GCATGC GCATG^C
Sse8387I 66 CCTGCAGG CCTGCA^GG
SspI 8452 AATATT AAT^ATT
Tth111I 8282 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2203 CCAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 1686 GTCTAC GT^[AC][GT]AC
3760 GTAGAC
AccII 2320 CGCG CG^CG
6164 CGCG
AccIII 1589 TCCGGA T^CCGGA
1979 TCCGGA
Asp700I 1954 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5608 GAAATGCTTC
BbeI 6081 GGCGCC GGCGC^C
8026 GGCGCC
BclI 177 TGATCA T^GATCA
518 TGATCA
BglII 200 AGATCT A^GATCT
1302 AGATCT
BsaBI 3917 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7793 GATCGAAATC
BsaWI 1589 TCCGGA [AT]^CCGG[AT]
1979 TCCGGA
BseAI 1589 TCCGGA T^CCGGA
1979 TCCGGA
BsiEI 2430 CGGCCG CG[GA][CT]^CG
6243 CGATCG
BspEI 1589 TCCGGA T^CCGGA
1979 TCCGGA
BspHI 4590 TCATGA T^CATGA
7259 TCATGA
BsrDI 911 GCAATG GCAATG(2/0)
1405 GCAATG
BstUI 2320 CGCG CG^CG
6164 CGCG
DraIII 1672 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
7951 CACAGGGTG
FbaI 177 TGATCA T^GATCA
518 TGATCA
KasI 6081 GGCGCC G^GCGCC
8026 GGCGCC
MamI 3917 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7793 GATCGAAATC
MroI 1589 TCCGGA T^CCGGA
1979 TCCGGA
MvnI 2320 CGCG CG^CG
6164 CGCG
NaeI 2432 GCCGGC GCC^GGC
7219 GCCGGC
NarI 6081 GGCGCC GG^CGCC
8026 GGCGCC
NgoMIV 2432 GCCGGC G^CCGGC
7219 GCCGGC
PaeR7I 3468 CTCGAG C^TCGAG
4833 CTCGAG
RcaI 4590 TCATGA T^CATGA
7259 TCATGA
SfoI 6081 GGCGCC GGC^GCC
8026 GGCGCC
XbaI 840 TCTAGA T^CTAGA
4570 TCTAGA
XhoI 3468 CTCGAG C^TCGAG
4833 CTCGAG
XmnI 1954 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5608 GAAATGCTTC
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# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
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AciI AcsI AcyI AfaI AhdI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI AseI AsnI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BssSI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI