Restriction map for
pFN21AE1495
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BmyI BsaAI BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstF5I BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 296 CGCG CG^CG
AcsI 673 AAATTC [GA]^AATT[CT]
AcyI 323 GGCGCC G[GA]^CG[CT]C
AlwI 111 GATCC GGATC(4/5)
Aor51HI 383 AGCGCT AGC^GCT
ApoI 673 AAATTC [GA]^AATT[CT]
BanI 323 GGCGCC G^G[CT][GA]CC
BbeI 323 GGCGCC GGCGC^C
BbsI 517 GAAGAC GAAGAC(2/6)
BglII 314 AGATCT A^GATCT
BlpI 265 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 265 GCTGAGC GC^T[ACGT]AGC
BpuAI 517 GAAGAC GAAGAC(2/6)
BsaHI 323 GGCGCC G[GA]^CG[CT]C
BsaWI 668 ACCGGA [AT]^CCGG[AT]
BseRI 79 GAGGAG GAGGAG(10/8)
BsmFI 65 GGGAC GGGAC(10/14)
BspMI 41 ACCTGC ACCTGC(4/8)
BsrDI 261 CATTGC GCAATG(2/0)
BssSI 230 CTCGTG CACGAG(-5/-1)
BstEII 621 GGTGACC G^GT[ACGT]ACC
BstPI 621 GGTGACC G^GT[ACGT]ACC
BstUI 296 CGCG CG^CG
BstXI 347 CCAACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 314 AGATCT [GA]^GATC[CT]
CelII 265 GCTGAGC GC^T[ACGT]AGC
DraII 65 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 503 CCACGG C^C[GA][CT]GG
EarI 19 CTCTTC CTCTTC(1/4)
Eco47III 383 AGCGCT AGC^GCT
EcoO109I 65 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 621 GGTGACC G^GT[ACGT]ACC
FauI 194 CCCGC CCCGC(4/6)
Hin1I 323 GGCGCC G[GA]^CG[CT]C
KasI 323 GGCGCC G^GCGCC
Ksp632I 19 CTCTTC CTCTTC(1/4)
MaeII 413 ACGT A^CGT
MflI 314 AGATCT [GA]^GATC[CT]
MseI 494 TTAA T^TAA
MvnI 296 CGCG CG^CG
NarI 323 GGCGCC GG^CGCC
NspI 529 GCATGC [GA]CATG^[CT]
PleI 641 GAGTC GAGTC(4/5)
PpuMI 65 GGGACCC [GA]G^G[AT]CC[CT]
SapI 18 GCTCTTC GCTCTTC(1/4)
SfaNI 93 GATGC GCATC(5/9)
SfoI 323 GGCGCC GGC^GCC
SmaI 576 CCCGGG CCC^GGG
SphI 529 GCATGC GCATG^C
TfiI 3 GAATC G^A[AT]TC
Tru9I 494 TTAA T^TAA
Tsp509I 674 AATT ^AATT
XcmI 503 CCACGGTGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 314 AGATCT [GA]^GATC[CT]
XmaCI 576 CCCGGG C^CCGGG
XmaI 576 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 66 GGACC G^G[AT]CC
180 GGACC
BpmI 206 CTGGAG CTGGAG(16/14)
217 CTGGAG
BsaI 149 GAGACC GGTCTC(1/5)
709 GAGACC
BsrBI 114 CCGCTC CCGCTC(-3/-3)
195 CCGCTC
BsrFI 270 GCCGGC [GA]^CCGG[CT]
647 GCCGGC
Cfr10I 270 GCCGGC [GA]^CCGG[CT]
647 GCCGGC
DdeI 97 CTGAG C^T[ACGT]AG
266 CTGAG
EaeI 272 CGGCCA [CT]^GGCC[GA]
361 TGGCCG
EcoT14I 656 CCAAGG C^C[AT][AT]GG
714 CCTTGG
HinfI 3 GAATC G^A[ACGT]TC
641 GAGTC
MspA1I 439 CAGCTG C[AC]G^C[GT]G
551 CAGCTG
NaeI 270 GCCGGC GCC^GGC
647 GCCGGC
NgoMIV 270 GCCGGC G^CCGGC
647 GCCGGC
PvuII 439 CAGCTG CAG^CTG
551 CAGCTG
StyI 656 CCAAGG C^C[AT][AT]GG
714 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BsgI BsiYI BslI BsmAI
BsoBI BssKI BstNI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
Tsp45I TspRI TthHB8I