Restriction map for
pFN21AE5412
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbsI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NarI NciI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AclI 220 AACGTT AA^CGTT
AcsI 25 GAATTT [GA]^AATT[CT]
AflIII 203 ACGTGT A^C[GA][CT]GT
AhdI 45 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 568 GTGCAC G^TGCAC
AlwI 535 GATCC GGATC(4/5)
ApaLI 568 GTGCAC G^TGCAC
ApoI 25 GAATTT [GA]^AATT[CT]
Asp700I 699 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 45 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 568 GTGCAC G[AT]GC[AT]^C
BbrPI 319 CACGTG CAC^GTG
BbvCI 151 CCTCAGC CCTCAGC(-5/-2)
BbvI 371 GCTGC GCAGC(8/12)
BmyI 568 GTGCAC G[AGT]GC[ACT]^C
BsaAI 319 CACGTG [CT]AC^GT[GA]
BsaBI 138 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 777 GAGACC GGTCTC(1/5)
BsaJI 36 CCTGGG C^C[ACGT][ACGT]GG
BseRI 213 CTCCTC GAGGAG(10/8)
BsiHKAI 568 GTGCAC G[AT]GC[AT]^C
Bsp1286I 568 GTGCAC G[AGT]GC[ACT]^C
BsrDI 306 CATTGC GCAATG(2/0)
BsrFI 367 GCCGGC [GA]^CCGG[CT]
BstXI 230 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 534 AGATCC [GA]^GATC[CT]
CfoI 678 GCGC GCG^C
Cfr10I 367 GCCGGC [GA]^CCGG[CT]
EaeI 365 TGGCCG [CT]^GGCC[GA]
Eam1105I 45 GACCTGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 350 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 371 GCTGC GC^[ACGT]GC
HapII 368 CCGG C^CGG
HhaI 678 GCGC GCG^C
HinP1I 678 GCGC G^CGC
HincII 895 GTCAAC GT[CT]^[GA]AC
HindII 895 GTCAAC GT[CT]^[GA]AC
HpaII 368 CCGG C^CGG
HphI 908 TCACC GGTGA(8/7)
ItaI 371 GCTGC GC^[ACGT]GC
MamI 138 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 761 TCTTC GAAGA(8/7)
MflI 534 AGATCC [GA]^GATC[CT]
MseI 608 TTAA T^TAA
MspI 368 CCGG C^CGG
NaeI 367 GCCGGC GCC^GGC
NdeI 917 CATATG CA^TATG
NgoMIV 367 GCCGGC G^CCGGC
NspI 674 GCATGC [GA]CATG^[CT]
PmaCI 319 CACGTG CAC^GTG
PmlI 319 CACGTG CAC^GTG
Psp1406I 220 AACGTT AA^CGTT
SphI 674 GCATGC GCATG^C
SspI 118 AATATT AAT^ATT
Tru9I 608 TTAA T^TAA
TseI 371 GCTGC G^C[AT]GC
Tsp45I 19 GTGAC ^GT[GC]AC
XhoII 534 AGATCC [GA]^GATC[CT]
XmnI 699 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 91 GCGG CCGC(-3/-1)
199 GCGG
AfaI 171 GTAC GT^AC
360 GTAC
BclI 101 TGATCA T^GATCA
128 TGATCA
BfaI 88 CTAG C^TAG
404 CTAG
BsgI 289 GTGCAG GTGCAG(16/14)
530 GTGCAG
BsmAI 256 GAGAC GTCTC(1/5)
777 GAGAC
BssKI 36 CCTGG ^CC[ACGT]GG
336 CCTGG
BstNI 36 CCTGG CC^[AT]GG
336 CCTGG
Cac8I 367 GCCGGC GC[ACGT]^[ACGT]GC
674 GCATGC
DraII 718 GGGTCCC [GA]G^G[ACGT]CC[CT]
751 GGGACCC
EcoO109I 718 GGGTCCC [GA]G^G[ACGT]CC[CT]
751 GGGACCC
EcoRII 36 CCTGG ^CC[AT]GG
336 CCTGG
FbaI 101 TGATCA T^GATCA
128 TGATCA
HaeIII 348 GGCC GG^CC
366 GGCC
MaeI 88 CTAG C^TAG
404 CTAG
MaeIII 19 GTGAC ^GT[ACGT]AC
223 GTTAC
MvaI 36 CCTGG CC^[AT]GG
336 CCTGG
PleI 162 GAGTC GAGTC(4/5)
183 GAGTC
PpuMI 718 GGGTCCC [GA]G^G[AT]CC[CT]
751 GGGACCC
RsaI 171 GTAC GT^AC
360 GTAC
ScrFI 36 CCTGG CC^[ACGT]GG
336 CCTGG
SfaNI 281 GATGC GCATC(5/9)
143 GCATC
SfcI 61 CTACAG C^T[GA][CT]AG
861 CTACAG
TspRI 292 CAGTG CA[GC]TG[ACGT][ACGT]^
783 CACTG
XcmI 266 CCATTTCCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
549 CCAGATTGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AvaII BsiYI BslI BsmFI
BstF5I Cfr13I DdeI DpnII FokI
HinfI MaeII MboI MnlI MslI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I TfiI Tsp509I