Restriction map for
pFN21AE5310
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MluI MroI MspI MunI
MvaI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AflII 900 CTTAAG C^TTAAG
Asp700I 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 371 TGGCCA TGG^CCA
BbsI 69 GAAGAC GAAGAC(2/6)
BclI 107 TGATCA T^GATCA
BfrI 900 CTTAAG C^TTAAG
BpuAI 69 GAAGAC GAAGAC(2/6)
BsaJI 113 CCGTGG C^C[ACGT][ACGT]GG
BsgI 290 GTGCAG GTGCAG(16/14)
Bsp1407I 176 TGTACA T^GTACA
BspHI 401 TCATGA T^CATGA
BspMI 458 ACCTGC ACCTGC(4/8)
BsrDI 349 GCAATG GCAATG(2/0)
BsrGI 176 TGTACA T^GTACA
BsrI 588 ACTGG ACTGG(1/-1)
BstF5I 725 CATCC GGATG(2/0)
BstYI 757 GGATCT [GA]^GATC[CT]
DraII 439 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 113 CCGTGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoO109I 439 GGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 107 TGATCA T^GATCA
FokI 725 CATCC GGATG(9/13)
HindIII 707 AAGCTT A^AGCTT
MaeII 776 ACGT A^CGT
MflI 757 GGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 818 CAGCTG C[AC]G^C[GT]G
NdeI 375 CATATG CA^TATG
NlaIV 439 GGGGCC GG[ACGT]^[ACGT]CC
PvuII 818 CAGCTG CAG^CTG
RcaI 401 TCATGA T^CATGA
SfaNI 724 GCATC GCATC(5/9)
SmlI 900 CTTAAG C^T[CT][GA]AG
SspBI 176 TGTACA T^GTACA
SspI 124 AATATT AAT^ATT
TaqI 511 TCGA T^CGA
TthHB8I 511 TCGA T^CGA
XhoII 757 GGATCT [GA]^GATC[CT]
XmnI 586 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 786 AAATTT [GA]^AATT[CT]
908 AAATTC
ApoI 786 AAATTT [GA]^AATT[CT]
908 AAATTC
BbvI 346 GCAGC GCAGC(8/12)
820 GCTGC
BsiYI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BslI 113 CCGTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
363 CCGCTATGTGG
BsmI 422 GCATTC GAATGC(1/-1)
462 GCATTC
Cfr13I 440 GGGCC G^G[ACGT]CC
701 GGGCC
Fnu4HI 346 GCAGC GC^[ACGT]GC
820 GCTGC
HphI 57 TCACC GGTGA(8/7)
110 TCACC
ItaI 346 GCAGC GC^[ACGT]GC
820 GCTGC
MseI 643 TTAA T^TAA
901 TTAA
MwoI 346 GCAGCAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
442 GCCTTCATAGC
PleI 72 GACTC GAGTC(4/5)
557 GACTC
PstI 326 CTGCAG CTGCA^G
388 CTGCAG
Sau96I 440 GGGCC G^G[ACGT]CC
701 GGGCC
SfcI 326 CTGCAG C^T[GA][CT]AG
388 CTGCAG
Tru9I 643 TTAA T^TAA
901 TTAA
TseI 346 GCAGC G^C[AT]GC
820 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI AluI AlwI DdeI DpnII
HaeIII HinfI MboI MboII MnlI
MslI NdeII NlaIII RsaI Sau3AI
TfiI Tsp509I TspRI