Restriction map for
pFN21AE0453
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BcgI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI Ksp632I KspI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TaqI
TfiI TspRI TthHB8I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 9 GGTACC G^GTACC
AciI 13 CCGC CCGC(-3/-1)
AocI 502 CCTAAGG CC^T[ACGT]AGG
Asp700I 241 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 9 GGTACC G^GTACC
AspHI 262 GAGCAC G[AT]GC[AT]^C
AspI 412 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 41 CCCGGG C^[CT]CG[GA]G
AvaII 625 GGACC G^G[AT]CC
BanI 9 GGTACC G^G[CT][GA]CC
BanII 151 GAGCCC G[GA]GC[CT]^C
BbvCI 646 CCTCAGC CCTCAGC(-5/-2)
BciVI 471 GTATCC GTATCC(6/5)
BfaI 638 CTAG C^TAG
BpmI 97 CTGGAG CTGGAG(16/14)
BsaI 447 GAGACC GGTCTC(1/5)
BsiHKAI 262 GAGCAC G[AT]GC[AT]^C
BsmFI 559 GTCCC GGGAC(10/14)
BsoBI 41 CCCGGG C^[CT]CG[GA]G
Bsp1407I 255 TGTACA T^GTACA
BspMI 659 GCAGGT ACCTGC(4/8)
BsrGI 255 TGTACA T^GTACA
Bsu36I 502 CCTAAGG CC^T[ACGT]AGG
Eco81I 502 CCTAAGG CC^T[ACGT]AGG
HaeIII 38 GGCC GG^CC
KpnI 9 GGTACC GGTAC^C
MaeI 638 CTAG C^TAG
MseI 370 TTAA T^TAA
MspA1I 292 CAGCTG C[AC]G^C[GT]G
PflFI 412 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 616 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 289 CTGCAG CTGCA^G
PvuII 292 CAGCTG CAG^CTG
SexAI 318 ACCTGGT A^CC[AT]GGT
SmaI 41 CCCGGG CCC^GGG
SspBI 255 TGTACA T^GTACA
Tru9I 370 TTAA T^TAA
Tsp45I 49 GTCAC ^GT[GC]AC
Tth111I 412 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 616 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
XmnI 241 GAAGGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 208 GAATTT [GA]^AATT[CT]
453 AAATTT
ApoI 208 GAATTT [GA]^AATT[CT]
453 AAATTT
BcnI 41 CCCGG CC^[GC]GG
42 CCGGG
BmyI 151 GAGCCC G[AGT]GC[ACT]^C
262 GAGCAC
BseRI 464 CTCCTC GAGGAG(10/8)
228 GAGGAG
BsgI 485 CTGCAC GTGCAG(16/14)
584 CTGCAC
BsiYI 90 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
616 CCAGGATATGG
BslI 90 CCTCACCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
616 CCAGGATATGG
BsmAI 447 GAGAC GTCTC(1/5)
641 GTCTC
Bsp1286I 151 GAGCCC G[AGT]GC[ACT]^C
262 GAGCAC
BsrFI 31 ACCGGC [GA]^CCGG[CT]
266 ACCGGC
BstF5I 72 CATCC GGATG(2/0)
396 GGATG
Cac8I 23 GCTCGC GC[ACGT]^[ACGT]GC
35 GCTGGC
CfoI 102 GCGC GCG^C
195 GCGC
Cfr10I 31 ACCGGC [GA]^CCGG[CT]
266 ACCGGC
Cfr13I 38 GGCCC G^G[ACGT]CC
625 GGACC
FokI 72 CATCC GGATG(9/13)
396 GGATG
HhaI 102 GCGC GCG^C
195 GCGC
HinP1I 102 GCGC G^CGC
195 GCGC
HphI 325 GGTGA GGTGA(8/7)
92 TCACC
MwoI 78 GCAGACGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
547 GCCTTGTTTGC
NciI 41 CCCGG CC^[GC]GG
42 CCGGG
Sau96I 38 GGCCC G^G[ACGT]CC
625 GGACC
SfcI 236 CTGTAG C^T[GA][CT]AG
289 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsrI
BssKI BstNI DdeI EcoRII Fnu4HI
HapII HpaII ItaI MaeIII MboII
MnlI MspI MvaI NlaIII NlaIV
RsaI ScrFI TseI Tsp509I