Restriction map for
pFN21AE5128
##################################
# NO CUT ENZYMES ( 185 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HapII
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MfeI MluI MluNI MroI MscI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AciI 208 CCGC CCGC(-3/-1)
AcyI 175 GACGCC G[GA]^CG[CT]C
AflIII 239 ACGTGT A^C[GA][CT]GT
AlwI 345 GATCC GGATC(4/5)
AlwNI 259 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 69 GGACC G^G[AT]CC
BbsI 220 GAAGAC GAAGAC(2/6)
BglI 115 GCCAATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 232 GTGCCC G[AGT]GC[ACT]^C
BpuAI 220 GAAGAC GAAGAC(2/6)
BsaAI 238 TACGTG [CT]AC^GT[GA]
BsaBI 13 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 175 GACGCC G[GA]^CG[CT]C
BsaI 282 GGTCTC GGTCTC(1/5)
BsgI 199 CTGCAC GTGCAG(16/14)
BsmAI 283 GTCTC GTCTC(1/5)
Bsp1286I 232 GTGCCC G[AGT]GC[ACT]^C
BspMI 203 ACCTGC ACCTGC(4/8)
BstYI 344 AGATCC [GA]^GATC[CT]
CfoI 249 GCGC GCG^C
DraII 260 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 260 AGGCCCT [GA]G^G[ACGT]CC[CT]
HgaI 175 GACGC GACGC(5/10)
HhaI 249 GCGC GCG^C
Hin1I 175 GACGCC G[GA]^CG[CT]C
HinP1I 249 GCGC G^CGC
HinfI 18 GAATC G^A[ACGT]TC
MaeII 239 ACGT A^CGT
MamI 13 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 220 GAAGA GAAGA(8/7)
MflI 344 AGATCC [GA]^GATC[CT]
MslI 159 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 86 CATG CATG^
NlaIV 112 GGAGCC GG[ACGT]^[ACGT]CC
PstI 170 CTGCAG CTGCA^G
PvuII 331 CAGCTG CAG^CTG
SfcI 170 CTGCAG C^T[GA][CT]AG
TfiI 18 GAATC G^A[AT]TC
Tsp45I 310 GTCAC ^GT[GC]AC
TspRI 185 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 344 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BpmI 285 CTCCAG CTGGAG(16/14)
187 CTGGAG
BsaJI 263 CCCTGG C^C[ACGT][ACGT]GG
264 CCTGGG
BsrI 66 ACTGG ACTGG(1/-1)
186 ACTGG
BssKI 264 CCTGG ^CC[ACGT]GG
287 CCAGG
BstNI 264 CCTGG CC^[AT]GG
287 CCAGG
Cac8I 258 GCAGGC GC[ACGT]^[ACGT]GC
326 GCTCGC
Cfr13I 69 GGACC G^G[ACGT]CC
261 GGCCC
DpnII 192 GATC ^GATC
345 GATC
EcoRII 264 CCTGG ^CC[AT]GG
287 CCAGG
HphI 53 TCACC GGTGA(8/7)
311 TCACC
MaeIII 89 GTAAC ^GT[ACGT]AC
310 GTCAC
MboI 192 GATC ^GATC
345 GATC
MspA1I 208 CCGCTG C[AC]G^C[GT]G
331 CAGCTG
MvaI 264 CCTGG CC^[AT]GG
287 CCAGG
NdeII 192 GATC ^GATC
345 GATC
Sau3AI 192 GATC ^GATC
345 GATC
Sau96I 69 GGACC G^G[ACGT]CC
261 GGCCC
ScrFI 264 CCTGG CC^[ACGT]GG
287 CCAGG
SfaNI 43 GATGC GCATC(5/9)
146 GCATC
StuI 26 AGGCCT AGG^CCT
289 AGGCCT
XcmI 56 CCAAGAAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
254 CCAAGCAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BbvI BsiYI BslI DdeI
Fnu4HI HaeIII ItaI MnlI MwoI
TseI